ABSTRACT
AIM: To evaluate the presence of the aggregated proteins in malignant and benign neoplasms for clarifying the role of impaired protein metabolism in the formation of the altered tissues. OBJECT AND METHODS: The histological specimens prepared from the operative materials of 196 patients with different forms of malignant and benign neoplasms were stained with Congo red and Thioflavin T and studied under the light and polarization microscope. RESULTS: The various forms of ß-stacked protein aggregates (ß-SPA) inclusions were detected in amyloids, keloid tissue, benign polyps, and several malignant tumors. CONCLUSION: The formation of non-functional protein aggregates proves the complex character of the impairment of protein metabolism resulting in local or systemic accumulation of secondary protein toxins results in ß-SPA formation as the self-sustaining complex of parametabolic processes. The ß-SPA formation is of considerable interest since their properties lead to the impairment of the normal physiological processes in adjacent tissues ensuring the chronic course of the pathology.
Subject(s)
Neoplasms/diagnosis , Neoplasms/metabolism , Protein Aggregates , Protein Aggregation, Pathological , Amyloid/metabolism , Biomarkers , Humans , Immunohistochemistry/methods , Neoplasm Grading , Neoplasms/surgeryABSTRACT
Modern methods (intrascopic and other instrumental) were used to increase the diagnostic efficiency and extension of the tumour process in 22 patients with lymphosarcoma of the pharynx. Optimal treatment efficacy is achieved in lymphosarcoma of the pharynx by combined use of surgical, radiation (non-traditional course) treatment as well as chemotherapy which is used at early stages as an adjunctive method.
Subject(s)
Lymphoma, Non-Hodgkin/diagnosis , Pharyngeal Neoplasms/diagnosis , Adolescent , Adult , Aged , Chemotherapy, Adjuvant , Combined Modality Therapy , Female , Humans , Lymphoma, Non-Hodgkin/pathology , Lymphoma, Non-Hodgkin/therapy , Male , Middle Aged , Nasopharyngeal Neoplasms/diagnosis , Nasopharyngeal Neoplasms/pathology , Nasopharyngeal Neoplasms/therapy , Neoplasm Staging , Oropharyngeal Neoplasms/diagnosis , Oropharyngeal Neoplasms/pathology , Oropharyngeal Neoplasms/therapy , Pharyngeal Neoplasms/pathology , Pharyngeal Neoplasms/therapy , Pharynx/surgery , Radiotherapy DosageSubject(s)
Carcinoma, Squamous Cell/pathology , Laryngeal Mucosa/cytology , Laryngeal Neoplasms/pathology , Larynx/cytology , Cell Division , Cell Nucleus/ultrastructure , Cells, Cultured , Epithelial Cells , Epithelium/pathology , Humans , Laryngeal Mucosa/pathology , Mitosis , Time Factors , Tumor Cells, CulturedABSTRACT
Fifty bacterial cultures were isolated from technical oils stored for different periods of time. Twenty out of the fifty strains decomposed technical oils. The bacteria were identified and classified as Bacillus strains. Bacillus subtilis, strains 7 and 10, and B. pumilus, strain 13, isolated from oils MK-8 + 10% Akor-1 and MT-16p as well as from AMG-10 were found to be most active and can be recommended as test cultures to study the resistance of technical oils against bacterial degradation.
Subject(s)
Bacillus/enzymology , Oils/metabolism , Bacillus/classification , Bacillus/isolation & purification , Biodegradation, Environmental , Climate , PetroleumSubject(s)
Labyrinth Diseases/pathology , Ossification, Heterotopic/pathology , Adult , Autopsy , Ear, Inner/pathology , Epilepsy/pathology , Humans , MaleSubject(s)
Liver Neoplasms/pathology , Skull Neoplasms/secondary , Temporal Bone/pathology , Aged , Bone Marrow/pathology , Female , HumansSubject(s)
Laryngeal Diseases/epidemiology , Adolescent , Adult , Aged , Carcinoma, Squamous Cell/epidemiology , Carcinoma, Squamous Cell/pathology , Child , Female , Fibroma/epidemiology , Fibroma/pathology , Humans , Laryngeal Diseases/pathology , Laryngeal Neoplasms/epidemiology , Laryngeal Neoplasms/pathology , Male , Middle Aged , Papilloma/epidemiology , Papilloma/pathology , Sex Ratio , UkraineSubject(s)
Bacteria/isolation & purification , Bacteriological Techniques , DNA, Bacterial , Nucleic Acid Hybridization , Alcaligenes/isolation & purification , Bacteria/analysis , DNA, Bacterial/analysis , Epidemiologic Methods , Escherichia coli/isolation & purification , Species Specificity , Time FactorsSubject(s)
Cell- and Tissue-Based Therapy , Cells, Cultured , Adult , Aged , Animals , Clinical Trials as Topic , Dimethyl Sulfoxide/therapeutic use , Embryo, Mammalian/cytology , Humans , Laryngeal Neoplasms/therapy , Laryngectomy , Middle Aged , Postoperative Care , Radiodermatitis/therapy , Rats , Regeneration/drug effectsABSTRACT
Base composition distribution of pyrimidine isopliths with general formula PyrnPn+1 in DNA of Actinomyces coelicolor 66 Kras. and in DNK of its actinophage F448 were investigater. These DNAs differ both pyrimidine isoplith distribution and in composition. The quality of shorter pyrimidine tracts in Actinomyces coelicolor DNA is higher compared with that in actinophage F448 DNA. The possibility of exogenic origin of actinophage F448 is discussed.
