ABSTRACT
BACKGROUND: The problem of transfusion-transmitted infections still remains serious and actual for health care despite the detailed testing of donors. Human immunodeficiency virus, hepatitis B and C viruses and human cytomegalovirus are among the most dangerous pathogens that can be transmitted with blood. Previously, a composition consisting of fullerene layer applied on silica gel particles was shown to inactivate influenza virus up to complete loss of infectivity. METHODS: In the present study the unit has been developed with source of irradiation whose spectrum is appropriate for solid-phase fullerene. The ability of the unit to inactivate the enveloped influenza virus in protein fraction of donor blood has been studied. RESULTS: It was shown that at optimized conditions complete inactivation of enveloped virus of extremely high initial titer (7.0-9.5 log 10 EID 50/0.2 mL) in the solution of albumin was achieved after as short time as 30 min of irradiation. This process did not affect the oxidative metabolism of neutrophils and membranes of erythrocytes evaluated by NBT reduction test and morphological analysis of erythrocytes, respectively. CONCLUSION: The data obtained suggests that the method described can be recommended for further development and optimization of the procedure of inactivation of viruses in the preparations of the plasma of donor blood.
Subject(s)
Blood Proteins/drug effects , Blood Proteins/radiation effects , Fullerenes/administration & dosage , Influenza A virus/drug effects , Influenza A virus/physiology , Virus Inactivation/drug effects , Virus Inactivation/radiation effects , Animals , Dogs , Fullerenes/chemistry , Influenza A virus/radiation effects , Madin Darby Canine Kidney Cells , Phase Transition , Photochemotherapy/methodsSubject(s)
Drug-Related Side Effects and Adverse Reactions , Peptic Ulcer Hemorrhage/chemically induced , Peptic Ulcer/complications , Adult , Aged , Aspirin/adverse effects , Aspirin/therapeutic use , Butadienes/adverse effects , Butadienes/therapeutic use , Female , Humans , Indomethacin/adverse effects , Indomethacin/therapeutic use , Male , Middle Aged , Prednisolone/adverse effects , Prednisolone/therapeutic use , Reserpine/adverse effectsABSTRACT
The author presents an analysis of the cause of bleeding in 37 patients and the genesis of development of erosions, acute ulcers of the mucous membrane of the stomach and duodenum (57 observations). Some recommendations on the diagnosis and prophylaxis of gastrointestinal hemorrhages are given.
