Permanent genetic resources added to Molecular Ecology Resources Database 1 December 2010-31 January 2011.

This article documents the addition of 238 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alytes dickhilleni, Arapaima gigas, Austropotamobius italicus, Blumeria graminis f. sp. tritici, Cobitis lutheri, Dendroctonus ponderosae, Glossina morsitans morsitans, Haplophilus subterraneus, Kirengeshoma palmata, Lysimachia japonica, Macrolophus pygmaeus, Microtus cabrerae, Mytilus galloprovincialis, Pallisentis (Neosentis) celatus, Pulmonaria officinalis, Salminus franciscanus, Thais chocolata and Zootoca vivipara. These loci were cross-tested on the following species: Acanthina monodon, Alytes cisternasii, Alytes maurus, Alytes muletensis, Alytes obstetricans almogavarii, Alytes obstetricans boscai, Alytes obstetricans obstetricans, Alytes obstetricans pertinax, Cambarellus montezumae, Cambarellus zempoalensis, Chorus giganteus, Cobitis tetralineata, Glossina fuscipes fuscipes, Glossina pallidipes, Lysimachia japonica var. japonica, Lysimachia japonica var. minutissima, Orconectes virilis, Pacifastacus leniusculus, Procambarus clarkii, Salminus brasiliensis and Salminus hilarii.

[Bladder infection by schistosoma: an uncommon cause of haematuria]. / Infección vesical por esquistosoma: una causa inhabitual de hematuria.

Immigration is a real phenomenon in our country with direct consequences into the public health system. In the attendance to these patients we do not have to forget the existence unusual diseases in our setting. We presented the clinical case of a patient with hematuria caused by bladder schistosomiasis.

Prevalencia del hábito tabáquico en trabajadores de oficinas de farmacia / Prevalence of smoking habit in pharmacy site workers

Introducción: Los profesionales de farmacia son un grupo de sanitariosen constante contacto con la población general. Por ello nuestroobjetivo fue estudiar la prevalencia del hábito tabáquico en trabajadoresde oficinas de farmacia según titulación, sexo y edad.Sujetos y método: Se estudió una muestra de 401 trabajadores delas 662 oficinas de farmacia existentes en Aragón, seleccionada por muestreobietápico. Se utilizó una encuesta anónima y autoadministrada que,dentro de un estudio más amplio, permitió clasificarlos en nunca fumadores,ex-fumadores y fumadores.Resultados: La prevalencia de fumadores fue del 36,91% (27,18%fumadores consonantes y 9,73% fumadores disonantes), registrándoseun 22,19% de ex-fumadores y un 40,90% de nunca fumadores. Poredad, hubo un 48,3% de fumadores menores de 30 años, un 37,1% teníanentre 30 y 45, un 29,1% entre 45 y 60, y un 21,7% mayores de60 años (p < 0,01). Por sexo, fumaba un 32,9% de los hombres y un39,0% de las mujeres (p < 0,001); y por titulación, fumaban un 29,4%de los titulados superiores, frente a un 46,8% de los auxiliares de farmacia(p < 0,0001).Conclusiones: La prevalencia de fumadores en trabajadores de oficinasde farmacia en Aragón es ligeramente superior a la de la poblacióngeneral aragonesa y española; disminuye con la edad, siendo mayor enmujeres, y en auxiliares de farmacia

Objectives: Chemist’s workers is a group of sanitation people whichare in constant touch with general population. This is the reason why westudy prevalence of smoking in a population of chemist’s workers, asa whole and according to degree, gender and age.Material and methods:We studied a sample of 401 chemist’s workers,obtained among 662 pharmacies in Aragon by two-phases sampling,giving them an anonymous and self-managed questionnaire, thatallowed us to classify workers, in a larger study, into three groups: neversmokers, ex-smokers or smokers.Results: The prevalence of smoking was of a 36,91% (27,18% ofconsonant smokers and 9,73% of dissonant smokers), being reported a22,19% of ex-smokers and a 40,90% of never smokers. In accordanceto age, they smoked 48,3% under 30 years, 37,1% between 30 and 45years, 29,1% between 45 and 60 years and 21,7% over 60 years (p <0,01). In accordance to gender, the prevalence of smoking was of a 32,9%in men and a 39,0% in women (p < 0,001); and in accordance to degree,they smoked 29,4% of those who have an universitary degree, and 46,8%among chemist’s assistants (p < 0,0001).Conclusions: The prevalence of smoking in chemist’s workers inAragon is lightly higher than in Spain and Aragon population, it decreaseswith age, and it’s higher in women and chemist’s assistants

Flow cytometric analysis of peroxidative activity in granulocytes from coronary and peripheral blood in acute myocardial ischemia and reperfusion in dogs: protective effect of methionine.

BACKGROUND: Methionine has shown protective effects in experimental models of myocardial infarction and is highly reactive to oxidative compounds produced by polymorphonuclear leukocytes (PMN), which in turn have been associated with myocardial damage. We have investigated the effect of methionine administration on spontaneous leukocyte peroxidative activity in myocardial ischemia and reperfusion. METHODS: In anesthetized dogs, with coronary occlusion (90 min) and reperfusion (90 min), PMN activation was measured by flow cytometric determination of H(2)O(2) with dihydrorhodamine 123, and correlated to hemodynamic parameters and infarct presence. To assess a possible direct effect of methionine, H(2)O(2) and superoxide were measured by flow cytometry in dog leukocyte suspensions following in vitro stimulation with f-MLP. RESULTS: PMN peroxidative activity in saline-treated dogs increased significantly after coronary occlusion and after reperfusion. These changes were greater in coronary venous blood than in femoral blood. Methionine administration (150 mg/kg, i.v.) before occlusion totally suppressed PMN activation, both after occlusion and reperfusion. CONCLUSIONS: PMN are promptly activated in myocardial ischemia, and methionine administration prevents such activation. However, methionine has no direct effect on spontaneous peroxidative activity, and f-MLP induced peroxidative activity. These in vivo effects of methionine, may additionally contribute to explain its protective role in experimental -788-877-7QQ8-8-7-88-8-8778--8Q78-----8--8-Q-7-Q7----- --------------8888 888888-7777777777777777777777777777777----------------888888888888888888 8877777--87--------8-----------------7-8888-887-----------8----8-8-87777 7777777------------------------------------------------------T7OW

Avaliaçäo microbiológica de refeiçöes servidas por empresas aéreas nacionais / Microbiological evaluation of food services in aviation

Avaliou-se a qualidade microbiológica de refeiçöes servidas por empresas aéreas nacionais, sendo constituídas por pratos à base de pescado, carnes e produtos cárneos, produtos lácteos e sobremesas, além da água utilizada nos locais de processamento dos alimentos e das mäos dos manipuladores. Foram determinadas contagens de bactérias mesófilas, de coliformes totais e E. coli e contagem e identificaçäo de S. aureus. Dos resultados obtidos constatou-se que todas as amostras de alimentos apresentavam contagens para bactérias mesófilas e coliformes totais abaixo dos limites máximos estabelecidos pelos padröes internacionais (RIBEIRO, 1974; ICMSF, 1982). A presença de E. coli em três amostras de queijo ricota, duas com valores 6,5 x 100 UFC/g e uma com valores maiores que 3,0 x 10.000 UFC/g, sendo estes resultados acima do permitido pela legislaçäo brasileira (BRASIL, 1997), demonstram a baixa qualidade do produto artesanal e as precárias condiçöes higiênico-sanitárias durante a produçäo. Verificou-se ausência de S. aureus em 100 por cento das amostras de alimentos analisados. Constatou-se, também, ausência de coliformes totais nas 10 (100 por cento) amostras de água e ausência de E. coli e S. aureus em todas as amostras das mäos dos manipuladores de alimentos. Os resultados encontrados sugerem a existência de condiçöes higiências adequadas durante o processamento e manipulaçäo das refeiçöes oferecidas pelas empresas aéreas nacionais, sendo próprias para consumo.

Immobilization of ocelots and bobcats with ketamine hydrochloride and xylazine hydrochloride.

We immobilized 10 ocelots (Felis pardalis), and 21 bobcats (F. rufus) in south Texas (USA) during March to November 1991 with a mixture of ketamine hydrochloride (KH) and xylazine hydrochloride (XH); two ocelots were immobilized twice. Species were immobilized with (mean +/- SE) 14.7 +/- 1.6 mg KH/kg body mass for ocelots, 13.3 +/- 1.8 mg KH/kg for bobcats, and 1.1 +/- 0.1 mg XH/kg and 1.2 +/- 0.1 mg XH/kg for ocelots and bobcats, respectively. Immobilization times in bobcats were longer (P = 0.08) than in ocelots. Adult female ocelots (18.5 +/- 2.6 mg/kg) needed larger (P < 0.05) doses of KH than adult males (12.0 +/- 1.7 mg/kg). Bobcats were immobilized during summer with lower initial (8.6 +/- 0.9 mg/kg, P < 0.001) and total (10.1 +/- 1.3 mg/kg, P = 0.02) doses of KH than bobcats immobilized in winter (14.5 +/- 1.0 mg/kg, and 18.5 +/- 3.8 mg/kg, respectively); summer immobilization times (44.3 +/- 3.8 min) were also shorter (P = 0.03) than during winter (59.1 +/- 5.2 min). Bobcats immobilized during summer had lower (P < 0.01) initial rectal temperatures (39.4 +/- 0.2 C) than bobcats trapped in winter (41.1 +/- 0.4 C). Overall, we observed no effects of KH-XH dose on body temperature.

Immobilization of coypus (Myocastor coypus) with ketamine hydrochloride and xylazine hydrochloride.

A combination of 100 mg/ml of ketamine hydrochloride (Ket) and 20 mg/ml of xylazine hydrochloride (Xyl) was used to immobilize coypus (Myocastor coypus). Eight mature coypus (four males and four females) were injected intramuscularly with doses ranging from 2.33 to 6.25 mg/kg of KET and 0.25 to 0.86 mg/kg of Xyl. The mean (+/- SE) time for induction, arousal, and recovery were 7.3 +/- 2 min, 23.5 +/- 0.3 min and 46 +/- 2.5 min, respectively. The mean +/- SE doses injected were 4.07 +/- 0.52 mg/kg Ket (range, 2.33 to 6.25 mg/kg) and 0.5 +/- 0.08 mg/kg Xyl (range, 0.25 to 0.86 mg/kg). No adverse responses were observed in any of the animals treated.

Immobilization of the endangered Iberian lynx with xylazine- and ketamine-hydrochloride.

A combination of the dissociative anesthetic ketamine hydrochloride (KH) and the sedative xylazine hydrochloride (XH) was used to immobilize 31 wild Iberian lynx (Felis pardina) 45 times at Doñana National Park, Spain. A mean (+/- SE) dose of 4.6 (+/- 0.2) mg/kg KH and 4.0 (+/- 0.2) mg/kg XH resulted in mean (+/- SE) induction time of 5.6 (+/- 0.3) min and mean (+/- SE) first reaction time of 59.3 (+/- 6.5) min. Convulsions occurred four times (9%), but with no noteworthy consequences.