ABSTRACT
We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells.
Subject(s)
Hydroxybutyrates/chemistry , Mesenchymal Stem Cells/physiology , Polyesters/chemistry , Tissue Scaffolds , Animals , Biocompatible Materials , Cell Differentiation , Cells, Cultured , Female , Mice, Inbred C57BL , Surface Properties , Tissue EngineeringABSTRACT
We studied umbilical cord blood mesenchymal stem cells and compared mesenchymal stem cells derived from umbilical cord blood, adipose tissue, and skin. Umbilical cord blood mesenchymal stem cells were characterized morphologically, cytofluorometrically, and by their differentiation potential. Umbilical cord blood mesenchymal stem cells did not differ from cells isolated from adipose tissue and skin by the main parameters (by morphology, expression of surface markers, and differentiation potential). A specific feature of umbilical cord blood mesenchymal stem cells is their low count per volume of the initial material and very low proliferative activity.
Subject(s)
Fetal Blood/cytology , Mesenchymal Stem Cells/cytology , Adipocytes/cytology , Adipose Tissue/cytology , Adult , Cell Differentiation , Cell Proliferation , Female , Humans , Osteoblasts/cytology , Skin/cytologyABSTRACT
In order to verify the relationship between the expression of Notch ligand in NIH 3T3 fibroblast clones and maintenance of hemopoietic activity in vitro, monolayers from three clones were co-cultured with mouse bone marrow mononuclears for 1-4 weeks and the total production of hemopoietic cells and myeloid colony-forming activity in methylcellulose were evaluated. One of the clones exhibited significant hemopoiesis maintaining activity and after 2 weeks of culturing promoted a more than 100-fold increase in the number of colony-forming precursors in comparison with control cells. Hemopoiesis maintenance activity of the second clone was much lower, and that of the third clone virtually did not differ from that of control cells. The results indicate that expression of Notch ligand can radically increase cell strain capacity to maintenance of in vitro hemopoiesis in the co-culturing system.
Subject(s)
Bone Marrow Cells/metabolism , Calcium-Binding Proteins/metabolism , Fibroblasts/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Membrane Proteins/metabolism , Animals , Bone Marrow Cells/cytology , Calcium-Binding Proteins/genetics , Cell Proliferation , Cells, Cultured , Coculture Techniques/methods , Colony-Forming Units Assay , Fibroblasts/cytology , Hematopoiesis , Hematopoietic Stem Cells/cytology , Hematopoietic Stem Cells/metabolism , Immunoblotting , Intercellular Signaling Peptides and Proteins/genetics , Jagged-1 Protein , Membrane Proteins/genetics , Mice , Mice, Inbred C57BL , NIH 3T3 Cells , Serrate-Jagged Proteins , Stromal Cells/cytology , Stromal Cells/metabolism , TransfectionSubject(s)
Bone Marrow Cells/metabolism , Gene Expression Profiling , Hematopoiesis/physiology , Signal Transduction/physiology , Tumor Necrosis Factor-alpha/physiology , Animals , Bone Marrow Cells/cytology , Cadherins/metabolism , Cell Differentiation , Cell Line , Cytokines/metabolism , Membrane Proteins/metabolism , Mice , Mice, Knockout , Osteopontin , Sialoglycoproteins/metabolism , Stromal Cells/cytology , Stromal Cells/metabolism , Tumor Necrosis Factor-alpha/geneticsABSTRACT
We compared differentiation potential of mesenchymal stem cells originating from human bone marrow, fatty tissue, thymus, placenta, and skin. The cells were characterized by differentiation into adipocytes and osteoblasts. Mesenchymal stem cells from different sources exhibited different differentiation potential, manifesting by the rate of differentiation and percentage of differentiated cells. Presumably, differentiation of mesenchymal stem cells derived from different tissues can differ due to the presence of progenitor cells of different types.
