Sensitivity comparison of laboratory-cultured and field-collected amphipod Corophium multisetosum in toxicity tests.

The feasibility of using lab-cultured amphipods Corophium multisetosum (Stock 1952) to evaluate the toxicity of contaminants present within marine sediments was studied. This was done by comparing the sensitivity of lab-cultured amphipods in a cadmium toxicity test and to toxic sediment samples, during a 10-days bioassay, with field collected individuals. Different responses were observed between field and cultured individuals. Cadmium test indicated high temporal variability in the LC(50) values of field amphipods (2.40-6.55 mg L(-1)). Sensitivity of cultured amphipods was within the seasonal range of the field individuals (5.81 mg L(-1), LC(50)). However, culture amphipods showed much lower sensitivity in toxic sediment samples. Our results indicate that sensitivity should be determined using a sediment matrix, if the assessment of toxicity is based upon bioassays performed with cultured burrower-amphipods.

Seasonal variability in gonad development in the sea urchin (Paracentrotus lividus) on the Basque coast (southeastern Bay of Biscay).

The main limiting factor to carrying out toxicological bioassays with sea urchin larvae is the restricted period for the availability of viable gametes. Although studies have been undertaken of the reproductive cycle of the sea urchin for several areas of the Bay of Biscay, only limited information exists for the southeasternmost area (the Basque coast). Furthermore, this geographical zone presents some particular environmental conditions, e.g., relatively warm waters. In this study, the gonad state of a population of Paracentrotus lividus, settled on the rocky shores of the city of Donostia-San Sebastian have been monitored. These sea urchins are observed to behave like a typical Atlantic population, with generally high gonad indices and a single yearly main spawning period, between April and May. Further, females show a shorter spawning period than males, becoming the limiting organisms for the availability of larvae.

Induction to maturation of the sea urchin Paracentrotus lividus (Lamarck, 1816) under laboratory conditions.

Laboratory experiments were carried out over two different periods, with the aim of investigating and utilizing the induction to gonadal maturation of the sea urchin Paracentrotus lividus. The final objective was to obtain viable gametes outside the period of natural spawning in the environment; which would allow the utilization of ecotoxicological bioassays with sea urchin larvae at any time of the year. The experiment consisted of maintaining some sea urchins in tanks and providing them with a natural photoperiod, unlimited food and a constant temperature of 20 degrees C. During days 0, 30, 60 and 90, gonads from 15 of these sea urchins were compared with those collected simultaneously in the natural environment. The gametes obtained were used to carry out fecundations, in order to check their viability. The final results obtained were clearly influenced by the gonadal state of the sea urchins at the initial stage of the experiment. The best results were obtained within a time period of 60 days and when the initial gonad index was low.

The effect of seasonality and body size on the sensitivity of marine amphipods to toxicants.

Two factors, seasonality and body size, were studied to determine their influence on the sensitivity of the amphipods Corophium urdaibaiense and Corophium multisetosum to toxicants. Seasonality was studied by comparing LC50 values for cadmium and ammonia toxicity to both species over a year. Body size effect was studied by comparing LC50 values of ammonia in three size categories of C. urdaibaiense. Except for the case of C. urdaibaiense with ammonia as a toxicant, the sensitivity was maximum during summer and minimum during winter. Furthermore, differences in sensitivities were found among the three body size groups studied.

Implementation of the European water framework directive from the Basque country (northern Spain): a methodological approach.

The European Water Framework Directive provides a challenge in the development of new and accurate methodologies. It addresses assessment of Ecological Quality Status within European rivers, lakes, groundwaters, estuaries and coasts. Although this directive is simple and flexible in its concept, it is necessary to develop an approach based upon scientific knowledge; however, at the same time it should be as simple as possible, in order to achieve both requirements and comparability of results throughout European waters. This contribution presents the first methodological approach to the problem, as used for estuaries and coasts of the Basque Country (northern Spain), in: selecting typologies and reference conditions; determining biological quality and ecological status; and identifying some problems in implementing the WFD. As such, the present paper could serve as the basis for a discussion document for other regions and countries, throughout Europe.