Spatial and temporal variation of biochemical biomarkers in Gobius niger (Gobiidae) from a southern Mediterranean lagoon (Bizerta lagoon, Tunisia): Influence of biotic and abiotic factors.

This study aims at evaluating both the influence of natural and some anthropogenic pressures on spatio-temporal variations on biomarker responses in sedentary benthic fish Gobius niger. For this purpose, variability of biotransformation enzymes and oxidative stress parameters response were studied in six stations from Bizerta lagoon as well as a reference station located in Ghar El Melh lagoon. Biomarker responses were shown to vary according to both physico-chemical parameters and anthropogenic pressures, but no influence of sex was reported. Based on multivariate analyses, the responses of biomarkers, obtained after covariate analysis in order to weigh the effect of physico-chemical parameters, allowed us to discriminate all stations, with a good classification rate for those that are highly contaminated. Altogether, this study shows the usefulness of G. niger as a sentinel species and stresses the necessity of integrating natural variables for data interpretation.

Environmental factors and seasonal dynamics of Prorocentrum lima population in coastal waters of the Gulf of Tunis, South Mediterranean.

In the frame of a phytoplankton study, a series of samples were collected in the Punic harbors of Carthage (Gulf of Tunis, South Mediterranean) from March 2008 to March 2010. Species composition, seasonal variations of pelagic Prorocentrum populations and several physico-chemical parameters were specified, as well as the relation between Prorocentrum species dynamics and water temperature, salinity and nutrient concentrations. The main detected species are included in the benthic/epiphytic group; P. lima (Ehrenberg) Stein 1878 and P. emarginatum Fukuyo 1981. P lima was the dominant species. The highest abundance of P. lima (6.97 x 10(3) Cells L(-1)) was recorded in August 2008 where temperature and salinity values ranged from 23.1 to 30.2 degrees C and 36.1 to 40.3 psu, respectively. The dynamics of the P. lima population had significant positive correlations with both temperature and salinity, and negative correlations with both nitrogen/phosphorus ratio and ammonium concentrations. Proliferations of this species represent a serious hazard for public health in Tunis northern coasts.

Kudoa azevedoi n. sp. (Myxozoa, Multivalvulida) from the oocytes of the Atlantic horse mackerel Trachurus trachurus (Perciformes, Carangidae) in Tunisian coasts.

A new species Kudoa azevedoi sp. n. (Myxozoa, Multivalvulida) is described in Trachurus trachurus Linnaeus, 1758 (Carangidae) from fishing harbors in Tunisian coasts using spore morphology and SSU rDNA sequence data. The parasite occurs only in ovaries within oocytes of mature and immature specimens. Spores are quadrate in shape in apical view with rounded edges, having four shell valves and four symmetrical polar capsules. They are of small sizes and measure 3.5±0.41 (3-4.2)×4.5±0.44 (4-5.2) length by width. The polar capsules are pyriform in shape measuring 1.5±0.22 (1.5-2)×0.75±0.14 (0.5-1) µm. Infected oocytes are hypertrophied, whitish colored, and filled with mature spores. Plasmodia are tubular and ramified from the inner membrane toward the center of the oocyte. Phylogenetic analysis based on small subunit ribosomal DNA sequences shows the highest similarity (96%) with the ovary parasite Kudoa ovivora. Some morphological details and spore dimensions support the creation of a new species in the genus Kudoa. Mean prevalence among examined females is of about 55.5%. It varies between localities and length of fish. The present myxosporea is the second Kudoa species reported in fish ovaries.

Spraguea lophii (Microsporidia) parasite of the teleost fish, Lophius piscatorius from Tunisian coasts: evidence for an extensive chromosome length polymorphism.

A microsporidian of the genus Spraguea was found parasitizing the nervous tissues of Lophius piscatorius collected from various localities in the Mediterranean coastal areas of Tunisia. The tissue localization, the infection focus aspect and sporal dimorphism are characteristics of Spraguea lophii species. Molecular data based on partial sequence of SSUrRNA encoding gene shows few nucleotide polymorphisms, compared to all described Spraguea isolates. Molecular karyotype obtained on pulsed field gel electrophoresis (1D-PFGE) shows a profile with 14 stained bands in the range of 230-880 kbp and a genome size estimated to 6.700 kbp. The rare cutter endonuclease MluI KARD 2-D-PFGE fingerprint shows an extensive chromosome length polymorphism, but the number of chromosome is unchanged and consists of 15 different molecules. The extensive chromosome length polymorphism is associated to a reduced number of genetic events.

Overlapping patterns of morphometric and genetic differentiation in the Mediterranean goby Pomatoschistus tortonesei Miller, 1968 (Perciformes, Gobiidae) in Tunisian lagoons.

The genetic and morphological variations of Pomatoschistus tortonesei Miller, 1968 were studied in samples collected from three Tunisian lagoons. The morphological analysis included 18 morphometric measurements and was based on linear discriminant analysis (LDA), whereas the genetic analysis was based on the 16S-rRNA and COI mitochondrial genes. Both analyses differentiated the populations and demonstrated consistently a well-supported differentiation between the western Mediterranean samples (Bizerta and Tunis South lagoons) and the eastern Mediterranean sample (El Bibane lagoon). The observed differentiation could be explained in terms of the geographic isolation of the various populations and the influence of environmental factors, which differ greatly between the different sites. The molecular results revealed that the populations are characterised by unique haplotypes which are well defined in relation to limited gene flow and restricted dispersal abilities. Additionally, it seems that local selective pressures have modelled biometrical variation. Morphological results can reflect a differential habitat use revealed in the cephalic features and a different response to hydrodynamic constraints developed in dissimilar dorsal and pelvic fin lengths.

Geography and host specificity: two forces behind the genetic structure of the freshwater fish parasite Ligula intestinalis (Cestoda: Diphyllobothriidae).

Parasite species with global distributions and complex life cycles offer a rare opportunity to study alternative mechanisms of speciation and evolution in a single model. Here, genealogy and genetic structure, with respect to geography and fish host preference, have been analyzed for Ligula intestinalis, a tapeworm affecting freshwater fish. The data analyzed consisted of 109 tapeworms sampled from 13 fish host species in 18 different localities on a macrogeographic scale. Two mitochondrial genes, cytochrome oxidase subunit I and cytochrome B, and the nuclear sequence of intergenic transcribed spacer 2 (ITS2) were used for the genetic reconstruction. Different evolutionary patterns were found at the local and at the global geographic scales. On a local scale, the flat genetic structure was mainly attributed to contiguous range expansion. Migrating birds are the most likely cause of the homogenisation of the whole population, preventing the creation of significant genetic barriers. By contrast, on a global scale, genetically distant and well-separated clusters are present in different geographic areas. Reproductive isolation was found even between clades living in sympatry and infecting the same definitive host, suggesting the existence of efficient biologically determined genetic barriers, and thus possibly separate species. Although the ITS2 sequences were found to display considerable intragenomic variability, their relationships were generally in good agreement with the topology derived from mitochondrial genes.

Anthobothrium altavelae sp. n. (Cestoda: Tetraphyllidea) from the spiny butterfly ray Gymnura altavela (Elasmobranchii: gymnuridae) in Tunisia.

Seven Gymnura altavela (Linnaeus, 1758) (Elasmobranchii, Myliobatiformes) caught off the Tunisian coast were examined for endoparasites during a three-year period (1995-1998). A phyllobothriid cestode new to science was found in the spiral intestine of all host specimens. The presence of a tetrabothridiate scolex, bothridia lacking an apical sucker, laciniate strobila, and possession of postvaginal testes are sufficient to place this species in the genus Anthobothrium Van Beneden, 1850. In this genus, we consider that only two species, both previously reported from carcharhiniform sharks, are valid: Anthobothrium cornucopia Van Beneden, 1850 and A. laciniatum Linton, 1890. Anthobothrium altavelae sp. n. can be distinguished from its congeners by its length, strobila morphology, and the number of testes. The taxonomic position of the species attributed to Anthobothrium parasitic in gymnurids is discussed.