Light-Induced Release of Bound Glucose-6-phosphate Dehydrogenase to the Stroma in Pea Chloroplasts.

Light affects the partitioning of glucose-6-phosphate dehydrogenase between thylakoids and stroma in the chloroplast. Illumination of intact chloroplasts changes the ratio between bound and free enzyme from approximately 1:1 to 1:2. Treatment with NADPH, inorganic phosphate, or high pH also results in release of the enzyme from isolated thylakoids.

Induction of "pore" formation in plant cell membranes by toluene.

Treatment with aqueous toluene-ethanol has been shown to induce "pore" formation in plant cell membranes. The evidence is as follows: [List: see text]While the principal experimental material was roots of Atriplex nummularia Lindl., the fact that similar results were also observed with leaves of Pisum sativum L. and with the alga Chlorella pyrenoidosa Chik. suggests that the phenomenon is general.Although the phenomenon of pore induction is qualitatively similar to that in microorganisms, the pores induced appear to be smaller. It is proposed that induced leakage could be the basis for the development of simple and rapid methods for plant biochemical studies.