ABSTRACT
Human cDNAs coding for angiogenin were isolated from Li7 hepatoma. Analysis of the nucleotide sequences of isolated cDNA clones and its comparison with recently published sequences of cDNA and human angiogenin gene permitted to suggest that an intron is present in the 5' region of the gene, dividing the coding and 5' untranslating regions. The size of the intron exceeds 1700 b.p. Up to now it has been known that the angiogenin gene is a single copy gene. However our results of blot-hybridization with genomic DNA of some mammals showed that there are 2-3 copies of the gene in their genomes. According to our results the angiogenin gene is conserved in mammalian genomes.
Subject(s)
Angiogenesis Inducing Agents/genetics , Base Sequence , Growth Substances/genetics , Proteins/genetics , Ribonuclease, Pancreatic , Sequence Homology, Nucleic Acid , Animals , Cloning, Molecular , DNA/genetics , Exons , Genome, Human , Humans , Molecular Sequence Data , Nucleic Acid HybridizationABSTRACT
Trials of the first Soviet live recombinant smallpox-hepatitis B vaccine (SHBV) in volunteers (20 men aged 18-20 years) showed its safety, good "take"-rate, and lower reactogenicity as compared with the standard smallpox vaccine (LIVP strain). Smallpox virus-neutralizing antibodies in response to SHBV were produced as well as in response to the smallpox vaccine. Revaccination of human subjects with smallpox vaccine and SHBV 45 days after the previous vaccination resulted in antibody booster to vaccinia virus. After two inoculations of SHBV at an interval of 45 days no anti-HBsAg antibodies were found for 3 months after the last vaccination. However, even a single vaccination with SHBV induced priming to HBsAg. This could be demonstrated after inoculation of the subjects vaccinated with SHBV with one dose of plasma hepatitis vaccine. In the subjects vaccinated with SHBV antibody in response to the plasma vaccine formed more frequently and in higher titres than in those prevaccinated with smallpox vaccine or placebo.
Subject(s)
Antigens, Viral/immunology , Hepatitis B virus/immunology , Hepatitis B/prevention & control , Smallpox Vaccine/adverse effects , Variola virus/immunology , Viral Hepatitis Vaccines/adverse effects , Adolescent , Adult , Antibodies, Viral/analysis , Antigens, Viral/analysis , Drug Evaluation , Hepatitis B Antibodies/analysis , Hepatitis B Surface Antigens/analysis , Humans , Male , Smallpox Vaccine/immunology , Time Factors , Vaccines, Inactivated/adverse effects , Vaccines, Inactivated/immunology , Vaccines, Synthetic/adverse effects , Vaccines, Synthetic/immunology , Viral Hepatitis Vaccines/immunologyABSTRACT
Isolation of genomic clones containing the human insulin gene by screening the human genomic library for this gene using the cDNA rat insulin probe is reported. The analysis of promoter-enhancer region and exons sequences has revealed their identity to analogous sequences determined earlier.
