Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Chirality ; 22(8): 726-33, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20143413

ABSTRACT

When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase.


Subject(s)
Catechin/analysis , Catechin/chemistry , Chromatography, High Pressure Liquid/methods , Circular Dichroism/methods , Malpighiaceae/chemistry , Plant Leaves/chemistry , Catechin/isolation & purification , Electrodes , Linear Models , Methanol/chemistry , Reproducibility of Results , Stereoisomerism
SELECTION OF CITATIONS
SEARCH DETAIL
...