ABSTRACT
The study aimed to examine the in vivo inhibition effect of cobalt ion and silibinin on metabolic enzymes such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR), and glutathione S-transferase (GST) and their in vitro inhibition effect on 6PGD. Twenty-four Wistar Albino rats weighing approximately 250-300 g were used in the study. The rats were divided into 4 groups as group 1 (control): isotonic serum (0.5 mL i.p), group 2 (cobalt): (150 mg kg/day cobalt), group 3 (silibinin): (100 mg/kg/day silibinin), group 4 (cobalt + silibinin). As a result of the in vivo applications, a statistically significant decrease was observed in the activities of G6PD (p < 0.05), 6PGD (p < 0.05), GR (p < 0.05), and GST (p < 0.05) enzymes in the groups that were administered cobalt compared to the control group. It was also found that the activities of G6PD (p < 0.05), 6PGD (p > 0.05), GR (p > 0.05), and GST (p > 0.05) enzymes increased in groups that were administered cobalt + silibinin compared to the group that was administered cobalt. As for in vitro applications, it was found that different Co2+ ions inhibited 6PGD enzyme which was obtained as a result of purification with IC50 = 346.6 µM value, while silibinin increased 6PGD enzyme activity within the concentration range of 100-750 µM by 40%. As a result, it was found that cobalt ions had an inhibition effect on G6PD, GR, and GST enzymes, which are vitally important for living metabolism, in vitro and in vivo and inhibited 6PGD enzyme activity in vitro, and silibinin increased these enzyme activities in vivo and 6PGD enzyme activity both in vivo and in vitro and decreased the inhibition effect.
ABSTRACT
OBJECTIVE: To determine whether the endocan level may be related to the severity of spinal cord injury. BACKGROUND: Several biomarkers were evaluated for this purpose, but endocan has never been studied before. It is correlated to endothelial dysfunction and ischemia, which are the characteristics of spinal cord injury in most cases. MATERIAL AND METHODS: A total of 21 male Sprague-Dawley rats weighing 300-350 g were randomly divided into three groups. In Group I, only a laminectomy was performed; in Group II, a mild SCI was performed after laminectomy; and, in Group III, a severe SCI was performed after laminectomy. At 48 h after the injury, after neurological assessment by Tarlov method, all animals were euthanized. A 5 cc blood sample was drawn for biochemical analysis, and spinal cord tissues were removed for histopathological examination. RESULTS: The difference between Groups I and III was statistically significant (p < 0.05). There was also a moderately positive correlation between the severity of SCI and the endocan level (r = 0.59, p < 0.05). CONCLUSION: The endocan level may be used as an indicator to determine prognosis after SCI (Tab. 1, Fig. 2, Ref. 24). Text in PDF www.elis.sk.
