ABSTRACT
INTRODUCTION: We examined the relationship between proinflammatory cytokines that occur in the inflammatory reaction in the intestine in Hirschsprung disease (HD) and Hirschsprung-associated enterocolitis (HAEC). METHODS: Thirty cases (M:27, F:3) operated on due to HD. The cases were divided into three groups: group 1 with pre and post operative EC, group 2 with post-operative, and group 3 with pre-operative EC. The intestinal segments were evaluated by immunohistochemistry for interleukin 1 beta (IL-1ß), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). RESULTS: IL-1ß staining was significantly higher in the ganglionic zone of groups with enterocolitis compared to the control group (p = 0.012). TNF-α staining in the transitional zone of Group 3 and IL-1ß staining in the ganglionic zone of Group 1 was significantly higher than the control group (p = 0.030, p = 0.020). CONCLUSION: In our study, older age at diagnosis and more than 20% IL-1ß staining in the ganglionic segment were found to be risk factors for HAEC. It is noteworthy that the increase in IL-1ß can be associated with HAEC.
Subject(s)
Enterocolitis , Hirschsprung Disease , Humans , Infant , Hirschsprung Disease/complications , Hirschsprung Disease/surgery , Hirschsprung Disease/pathology , Tumor Necrosis Factor-alpha , Enterocolitis/etiology , Enterocolitis/pathology , Enterocolitis/surgery , Inflammation , Risk FactorsABSTRACT
Mixtures of boron and azomethine-H in solution result in slow complexation. Addition of sodium dodecyl sulfate (SDS), polyethylene glycol dodecyl ether (Brij-35), 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (TritonX-100), and cetyltrimethyl ammonium bromide (CTAB) result in considerable decrease in complexation time and enhancement in signal of peak in solution and also sol-gel. The fluorescence of the complex is monitored at an emission wavelength of 486 nm with excitation at 416 nm. The presence of 1x10(-3) mol L(-1) SDS decreased the complexation time up to 10 min in solution and 20 min in sol-gel for above 0.25 microg B mL(-1) and 30 min in solution and 35 min in sol-gel for below 0.25 microg B mL(-1). However, the photostability did not change by adding micelle in both media. The proposed method shows a linear response toward boron in the concentration range of 0.05-10 microg mL(-1) and is selective for boron over a large number of electrolytes and cations. The detection limit was 7 microg L(-1). This method has been used for the detection of boron in environmental water samples and fruit juices with satisfactory results.
