1.
Vopr Med Khim
; 36(1): 27-31, 1990.
Article
in Russian
| MEDLINE
| ID: mdl-2111600
ABSTRACT
Diphtheritic bacteria of PW-8 Massachusetts strain produced into cultural medium only one nucleotidase--endoDNAase. The enzyme was synthesized by the cells during the exponential phase of growth. The DNAase was purified 500-fold and exhibited properties specific to neutral-alkaline DNAases (pH optimum about 7.5, absolute requirements for Me2+, single-step mechanism of substrate hydrolysis). The following properties were typical for the enzyme: absence of distinct specificity to structure of bases surrounding the hydrolyzed bond, formation of 5'-end phosphate groups and slightly higher preference to denatured DNA.
Subject(s)
Corynebacterium diphtheriae/enzymology , Deoxyribonucleases/biosynthesis , Corynebacterium diphtheriae/growth & development , DNA, Bacterial/metabolism , Deoxyribonucleases/antagonists & inhibitors , Deoxyribonucleases/metabolism , Enzyme Activation , Hydrogen-Ion Concentration , Hydrolysis , Kinetics , Viscosity
2.
Biofizika
; 14(1): 5-13, 1969.
Article
in Russian
| MEDLINE
| ID: mdl-5397644