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1.
J Thromb Thrombolysis ; 29(3): 326-39, 2010 Apr.
Article in English | MEDLINE | ID: mdl-19548071

ABSTRACT

The 7th conference of the American College of Chest Physicians (ACCP7) provides recommendations on the type, dose, and duration of thromboprophylaxis in hospitalized patients at risk of venous thromboembolism (VTE), but the extent to which hospitals follow these criteria has not been well studied. Discharge and billing records for patients admitted to any of 16 acute-care hospitals from January 2005 to December 2006 were obtained. Patients 18 years or older who had an inpatient stay >or=2 days and no apparent contraindications for thromboprophylaxis were grouped into the categories of critical care, surgery and medically ill before being assessed for additional VTE risk factors based on the diagnostic criteria outlined in ACCP7. For patients at risk, the recommended type (mechanical or pharmacologic), dose, and duration of thromboprophylaxis was identified based on the guidelines and compared to the regimen actually received, if any. Among the 258,556 hospitalized patients, 68,278 (26.4%) were determined to be at risk of VTE without apparent contraindications for thromboprophylaxis. The proportions of patients who received the appropriate type, dose, and duration of thromboprophylaxis were 10.5, 9.8, and 17.9% for critical care, medical, and surgical patients, respectively. Of those at risk, 36.8% received no thromboprophylaxis and an additional 50.2% received thromboprophylaxis deemed inappropriate for one or more reasons. The implementation of ACCP7 guidelines for type, dosage, and duration of thromboprophylaxis is low in patients at risk of VTE. There is a need for physicians and health systems to improve awareness and implementation of recommended thromboprophylaxis.


Subject(s)
Delivery of Health Care/trends , Hospitals/trends , Practice Guidelines as Topic , Thrombolytic Therapy/trends , Venous Thromboembolism/prevention & control , Adolescent , Adult , Aged , Anticoagulants/therapeutic use , Female , Humans , Male , Middle Aged , Venous Thromboembolism/epidemiology , Young Adult
2.
Int J Clin Pract ; 62(10): 1484-98, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18691228

ABSTRACT

AIMS: We assessed whether a novel programme to evaluate/communicate predicted coronary heart disease (CHD) risk could lower patients' predicted Framingham CHD risk vs. usual care. METHODS: The Risk Evaluation and Communication Health Outcomes and Utilization Trial was a prospective, controlled, cluster-randomised trial in nine European countries, among patients at moderate cardiovascular risk. Following baseline assessments, physicians in the intervention group calculated patients' predicted CHD risk and were instructed to advise patients according to a risk evaluation/communication programme. Usual care physicians did not calculate patients' risk and provided usual care only. The primary end-point was Framingham 10-year CHD risk at 6 months with intervention vs. usual care. RESULTS: Of 1103 patients across 100 sites, 524 patients receiving intervention, and 461 receiving usual care, were analysed for efficacy. After 6 months, mean predicted risks were 12.5% with intervention, and 13.7% with usual care [odds ratio = 0.896; p = 0.001, adjusted for risk at baseline (17.2% intervention; 16.9% usual care) and other covariates]. The proportion of patients achieving both blood pressure and low-density lipoprotein cholesterol targets was significantly higher with intervention (25.4%) than usual care (14.1%; p < 0.001), and 29.3% of smokers in the intervention group quit smoking vs. 21.4% of those receiving usual care (p = 0.04). CONCLUSIONS: A physician-implemented CHD risk evaluation/communication programme improved patients' modifiable risk factor profile, and lowered predicted CHD risk compared with usual care. By combining this strategy with more intensive treatment to reduce residual modifiable risk, we believe that substantial improvements in cardiovascular disease prevention could be achieved in clinical practice.


Subject(s)
Cardiovascular Agents/therapeutic use , Coronary Disease/prevention & control , Clinical Protocols , Cluster Analysis , Communication , Coronary Disease/etiology , Coronary Disease/mortality , Death, Sudden, Cardiac/etiology , Female , Humans , Male , Middle Aged , Myocardial Infarction/etiology , Prognosis , Prospective Studies , Risk Assessment , Risk Factors , Weight Loss
3.
Int J Clin Pract ; 59(12): 1441-51, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16351677

ABSTRACT

The MEDLINE database was searched from 1972 to June 2002 to identify studies of interventions designed to improve compliance with antihypertensive or lipid-lowering medications. Studies were required to employ a controlled design, follow patients for >or=6 months and measure compliance by a method other than patient self-report. The literature review yielded 62 studies describing 79 interventions. Overall, 56% of interventions were reported to improve patient compliance. When only those studies meeting minimum criteria for methodological quality were considered, 22 interventions remained and 12 were recommended, because they demonstrated a significant improvement in compliance. Recommended interventions included fixed-dose combination drugs, once-daily or once-weekly dosing schedules, unit-dose packaging, educational counselling by telephone, case management by pharmacists, treatment in pharmacist- or nurse-operated disease management clinics, mailed refill reminders, self-monitoring, dose-tailoring, rewards and various combination strategies. Personalised, patient-focused programs that involved frequent contact with health professionals or a combination of interventions were the most effective at improving compliance. Less-intensive strategies, such as prescribing products that simplify the medication regimen or sending refill reminders, achieved smaller improvements in compliance but may be cost-effective due to their low cost.


Subject(s)
Antihypertensive Agents/administration & dosage , Cardiovascular Diseases/prevention & control , Hypolipidemic Agents/administration & dosage , Patient Compliance/statistics & numerical data , Ambulatory Care , Case Management , Counseling , Drug Administration Schedule , Drug Packaging , Drug Therapy, Combination , Humans , Medical Records , Reminder Systems , Telephone
4.
Biochemistry ; 39(11): 3097-105, 2000 Mar 21.
Article in English | MEDLINE | ID: mdl-10715131

ABSTRACT

Fenton chemistry [Fenton (1894) J. Chem. Soc. 65, 899-910] techniques were employed to identify the residues involved in metal binding located at the active sites of restriction endonucleases. This process uses transition metals to catalytically oxidize the peptide linkage that is in close proximity to the amino acid residues involved in metal ligation. Fe2+ was used as the redox-active transition metal. It was expected that Fe2+ would bind to the endonucleases at the Mg2+-binding site [Liaw et al. (1993) Biochemistry 32, 7999-4003; Ermácora et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6383-6387; Soundar and Colman (1993) J. Biol. Chem. 268, 5264-5271; Wei et al. (1994) Biochemistry 33, 7931-7936; Ettner et al. (1995) Biochemistry 34, 22-31; Hlavaty and Nowak (1997) Biochemistry 36, 15515-15525). Fe2+-mediated oxidation was successfully performed on TaqI endonulease, suggesting that this approach could be applied to a wide array of endonucleases [Cao and Barany (1998) J. Biol. Chem. 273, 33002-33010]. The restriction endonucleases BamHI, FokI, BglI, BglII, PvuII, SfiI, BssSI, BsoBI, EcoRI, EcoRV, MspI, and HinP1I were subjected to oxidizing conditions in the presence of Fe2+ and ascorbate. All proteins were inactivated upon treatment with Fe2+ and ascorbate. BamHI, FokI, BglI, BglII, PvuII, SfiI, BssSI, and BsoBI were specifically cleaved upon treatment with Fe2+/ascorbate. The site of Fe2+/ascorbate-induced protein cleavage for each enzyme was determined. The Fe2+-mediated oxidative cleavage of BamHI occurs between residues Glu77 and Lys78. Glu77 has been shown by structural and mutational studies to be involved in both metal ligation and catalysis [Newman et al. (1995) Science 269, 656-663; Viadiu and Aggarwal (1998) Nat. Struct. Biol. 5, 910-916; Xu and Schildkraut (1991) J. Biol. Chem. 266, 4425-4429]. The sites of Fe2+/ascorbate-induced cleavage for PvuII, FokI, BglI, and BsoBI agree with the metal-binding sites identified in their corresponding three-dimensional structures or from mutational studies [Cheng et al. (1994) EMBO J. 13, 3297-3935; Wah et al. (1997) Nature 388, 97-100; Newman et al. (1998) EMBO J. 17, 5466-5476; Ruan et al. (1997) Gene 188, 35-39]. The metal-binding residues of BglII, SfiI, and BssSI are proposed based on amino acid sequencing of their Fe2+/ascorbate-generated cleavage fragments. These results suggest that Fenton chemistry may be a useful methodology in identifying amino acids involved in metal binding in endonucleases.


Subject(s)
Deoxyribonucleases, Type II Site-Specific/chemistry , Iron/chemistry , Metals, Heavy/chemistry , Alanine/genetics , Amino Acid Sequence , Ascorbic Acid/chemistry , Aspartic Acid/genetics , Binding Sites/genetics , Deoxyribonuclease BamHI/chemistry , Deoxyribonucleases, Type II Site-Specific/genetics , Enzyme Activation/genetics , Free Radical Scavengers/chemistry , Hydrogen Peroxide/chemistry , Hydrolysis , Molecular Sequence Data , Mutagenesis, Site-Directed , Oxidation-Reduction
5.
Gene ; 187(1): 19-27, 1997 Mar 10.
Article in English | MEDLINE | ID: mdl-9073062

ABSTRACT

Bg/II, a type II restriction-modification (R-M) system from Bacillus globigii, recognizes the sequence 5'-AGATCT-3'. The system has been cloned into E. coli in multiple steps: first the methyltransferase (MTase) gene, bglIIM, was cloned from B. globigii RUB561, a variant containing an inactivated endonuclease (ENase) gene (bglIIR). Next the ENase protein (R.BglII) was purified to homogeneity from RUB562, a strain expressing the complete R-M system. Oligonucleotide probes specific for the 5' end of the gene were then synthesized and used to locate bglIIR, and the gene was isolated and cloned in a subsequent step. The nucleotide sequence of the system has been determined, and several interesting features have been found. The genes are tandemly arranged, with bglIIR preceding bglIIM. The amino acid sequence of M.BglII is compared to those of other known MTases. A third gene encoding a protein with sequence similarity to known C elements of other R-M systems is found upstream of bglIIR. This is the first instance of a C gene being associated with an R-M system where the R and M genes are collinear. In addition, open reading frames (ORFs) resembling genes involved with DNA mobility are found in close association with BglII. These may shed light on the evolution of the R-M system.


Subject(s)
Bacillus/enzymology , Bacterial Proteins , Cloning, Molecular , DNA Restriction-Modification Enzymes/genetics , Deoxyribonucleases, Type II Site-Specific/genetics , Evolution, Molecular , Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics , Amino Acid Sequence , Base Composition , Base Sequence , DNA Primers/chemistry , DNA Restriction-Modification Enzymes/chemistry , DNA, Bacterial/genetics , Deoxyribonucleases, Type II Site-Specific/chemistry , Electrophoresis, Agar Gel , Escherichia coli/genetics , Gene Expression , Genes, Bacterial , Molecular Sequence Data , Open Reading Frames , Restriction Mapping , Sequence Alignment , Sequence Analysis , Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry
6.
Gene ; 180(1-2): 107-12, 1996 Nov 21.
Article in English | MEDLINE | ID: mdl-8973353

ABSTRACT

SphI, a type II restriction-modification (R-M) system from the bacterium Streptomyces phaeochromogenes, recognizes the sequence 5'-GCATGC. The SphI methyltransferase (MTase)-encoding gene, sphIM, was cloned into Escherichia coli using MTase selection to isolate the clone. However, none of these clones contained the restriction endonuclease (ENase) gene. Repeated attempts to clone the complete ENase gene along with sphIM in one step failed, presumably due to expression of SphI ENase gene, sphIR, in the presence of inadequate expression of sphIM. The complete sphIR was finally cloned using a two-step process. PCR was used to isolate the 3' end of sphIR from a library. The intact sphIR, reconstructed under control of an inducible promoter, was introduced into an E. coli strain containing a plasmid with the NlaIII MTase-encoding gene (nlaIIIM). The nucleotide sequence of the SphI system was determined, analyzed and compared to previously sequenced R-M systems. The sequence was also examined for features which would help explain why sphIR unlike other actinomycete ENase genes seemed to be expressed in E. coli.


Subject(s)
Bacterial Proteins , DNA Modification Methylases/genetics , Deoxyribonucleases, Type II Site-Specific/genetics , Streptomyces/enzymology , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Bacterial , Escherichia coli , Gene Expression , Molecular Sequence Data , Restriction Mapping , Streptomyces/genetics
7.
Mol Biochem Parasitol ; 82(1): 51-65, 1996 Nov 12.
Article in English | MEDLINE | ID: mdl-8943150

ABSTRACT

A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into two related families. The consensus repeats, DiPA-CR1 and CR2, derived for each family are 80% identical. However, the repeats at the C-terminus of the polyprotein have diverged from DiPA-CR1 and CR2. This was shown by DNA sequence and Southern blot analysis of a 1.9 kb cDNA clone that encodes 4.4 C-terminal repeats (DiPA-TR1 through TR5). DiPA-TR3 through TR5 show 27-52% amino acid identity with the consensus repeats and 31-35% amino acid identity with one another. Metabolic labeling studies have shown that cleavage of DiPA generates a protein "ladder' from 14 to > 200 kDa. RRKR, a cleavage motif of subtilisin-like proprotein convertases, was identified as the natural cleavage site. In vitro digestion experiments with proteinase K suggest a structural model for DiPA consisting of protease resistant cores joined by protease sensitive linkers containing the RRKR site. This motif is absent between DiPA-TR3 and TR4 and has been altered to KR between DiPA-TR4 and TR5. An immunoblot of D. immitis extract probed with anti-DiPA-TR4/5 serum demonstrates the absence of cleavage at these sites. These divergent repeats provide an opportunity to investigate processing of the D. immitis polyprotein in vivo.


Subject(s)
Dirofilaria immitis/chemistry , Helminth Proteins/chemistry , Proteins/chemistry , Amino Acid Sequence , Animals , Antigens, Helminth/chemistry , Antigens, Helminth/metabolism , Base Sequence , Consensus Sequence , Helminth Proteins/metabolism , Immunoblotting , Models, Molecular , Molecular Sequence Data , Molecular Weight , Open Reading Frames , Protein Processing, Post-Translational , Proteins/metabolism , Recombinant Proteins/chemistry , Repetitive Sequences, Nucleic Acid , Sequence Alignment
8.
Genet Anal ; 12(5-6): 185-95, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8740835

ABSTRACT

A thermostable DNA polymerase, the Bst DNA polymerase I, from Bacillus stearothermophilus N3468 was prepared to near-homogeneity. The dominant species of the Bst DNA polymerase I preparation sized about 97 kDa when analyzed on SDS polyacrylamide gels. The Bst polA gene that codes for Bst polymerase I was cloned and sequenced. Comparative sequence analysis showed that all three conserved 3'-->5' exonuclease motifs found in E. coli DNA polymerase I were missing in Bst DNA polymerase I. This cast doubt on the existence of a 3'-->5' exonuclease function in that enzyme. Four biochemical assays were used to measure exonuclease activities of Bst DNA polymerase I, testing both full-length Bst polymerase I and the Bst large fragment which lacks the N-terminal 5'-->3' exonuclease domain. These exonuclease assays demonstrated that Bst DNA polymerase I only contained a double-strand dependent 5'-->3' exonuclease activity but lacked any detectable 3'-->5' proofreading exonuclease activity. The lack of 3'-->5' exonuclease function in a variety of thermostable repair DNA polymerases may reflect enhancement of thermostability at the expense of proofreading activity.


Subject(s)
DNA Polymerase I/metabolism , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA Polymerase I/genetics , DNA Polymerase I/isolation & purification , DNA Primers/genetics , DNA Repair , DNA, Bacterial/genetics , DNA, Bacterial/metabolism , Enzyme Stability , Escherichia coli/enzymology , Escherichia coli/genetics , Exodeoxyribonuclease V , Exodeoxyribonucleases/genetics , Exodeoxyribonucleases/isolation & purification , Exodeoxyribonucleases/metabolism , Genes, Bacterial , Geobacillus stearothermophilus/enzymology , Geobacillus stearothermophilus/genetics , Molecular Sequence Data , Molecular Weight , Sequence Homology, Amino Acid , Temperature
9.
Glycobiology ; 5(6): 603-10, 1995 Sep.
Article in English | MEDLINE | ID: mdl-8563148

ABSTRACT

The gene encoding a beta-galactosidase from Xanthomonas manihotis was cloned into Escherichia coli. The gene resides on a 2.4 kb DNA fragment which was isolated from a partial Sau3A library in the cloning vector pUC19 using 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) as the selection. The enzyme produced by the clone has a specificity for beta 1-3- > beta 1-4-linked galactose. The nucleotide sequence of the gene was determined. The deduced protein sequence contained 597 amino acids yielding a monomeric molecular mass of 66 kDa. The cloned beta-galactosidase showed no similarity to any known prokaryotic beta-galactosidase. However, extensive similarity was observed with eukaryotic beta-galactosidases from animals, plants and fungi. The strongest similarity was with the beta-galactosidases found in the human and mouse lysosomes (42 and 41% identity, respectively). Alignment of the X.manihotis and eukaryotic beta-galactosidase sequences revealed seven highly conserved domains common to each protein. Additionally, Domain 1 in X.manihotis showed similarity to regions within catalytic domains from seven xylanases and cellulases belonging to family 10 of glucosyl hydrolases. A region spanning Domain 2 showed similarity to the catalytic domain of endo beta 1-3 glucanases from tobacco and barley.


Subject(s)
Xanthomonas/genetics , beta-Galactosidase/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA, Bacterial , Escherichia coli/genetics , Humans , Molecular Sequence Data , Sequence Homology, Amino Acid , Xanthomonas/enzymology , beta-Galactosidase/metabolism
10.
Gene ; 155(1): 19-25, 1995 Mar 21.
Article in English | MEDLINE | ID: mdl-7698663

ABSTRACT

NaeI, a type-II restriction-modification (R-M) system from the bacterium Nocardia aerocolonigenes, recognizes the sequence 5'-GCCGGC. The NaeI DNA methyltransferase (MTase)-encoding gene, naeIM, had been cloned previously in Escherichia coli [Van Cott and Wilson, Gene 74 (1988) 55-59]. However, none of these clones expressed detectable levels of the restriction endonuclease (ENase). The absence of the intact ENase-encoding gene (naeIR) within the isolated MTase clones was confirmed by recloning the MTase clones into Streptomyces lividans. The complete NaeI system was finally cloned using E. coli AP1-200 [Piekarowicz et al., Nucleic Acids Res. 19 (1991) 1831-1835] and less stringent MTase-selection conditions. The naeIR gene was expressed first by cloning into S. lividans, and later by cloning under control of a regulated promoter in an E. coli strain preprotected by the heterologous MspI MTase (M.MspI). The DNA sequence of the NaeI R-M system has been determined, analyzed and compared to previously sequenced R-M systems.


Subject(s)
Deoxyribonucleases, Type II Site-Specific/chemistry , Deoxyribonucleases, Type II Site-Specific/genetics , Genes, Bacterial , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Deoxyribonucleases, Type II Site-Specific/biosynthesis , Escherichia coli/genetics , Molecular Sequence Data , Nocardia/genetics , Recombinant Proteins/biosynthesis , Sequence Homology, Amino Acid , Streptomyces/genetics
11.
Gene ; 150(1): 75-80, 1994 Dec 02.
Article in English | MEDLINE | ID: mdl-7959067

ABSTRACT

The genes encoding the HindIII restriction endonuclease (R.HindIII ENase) and methyltransferase (M.HindIII MTase) from Haemophilus influenzae Rd were cloned and expressed in Escherichia coli and their nucleotide (nt) sequences were determined. The genes are transcribed in the same orientation, with the ENase-encoding gene (hindIIIR) preceding the MTase-encoding gene (hindIIIM). The two genes overlap by several nt. The ENase is predicted to be 300 amino acids (aa) in length (34,950 Da); the MTase is predicted to be 309 aa (35,550 Da). The HindIII ENase and MTase activities increased approx. 20-fold when the genes were brought under the control of an inducible lambda pL promoter. Highly purified HindIII ENase and MTase proteins were prepared and their N-terminal aa sequences determined. In H. influenzae Rd, the HindIII R-M genes are located between the holC and valS genes; they are not closely linked to the HindII R-M genes.


Subject(s)
Deoxyribonuclease HindIII/genetics , Haemophilus influenzae/genetics , Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Bacterial , Escherichia coli , Genes, Bacterial , Haemophilus influenzae/enzymology , Molecular Sequence Data
12.
J Biol Chem ; 269(1): 683-90, 1994 Jan 07.
Article in English | MEDLINE | ID: mdl-8276869

ABSTRACT

The BcgI restriction enzyme from Bacillus coagulans is unusual in that it cleaves on both sides of its recognition site, CGAN6TGC, releasing a fragment that includes the site and several bases on each side. We report the organization and nucleotide sequences of the genes for the BcgI restriction-modification system and the properties of the proteins that they encode. The system comprises two adjacent, similarly oriented genes. The proximal gene, bcgIA, codes for a 637-amino acid protein (molecular mass = 71.6 kDa) that resembles certain m6A-specific DNA-methyltransferases, particularly those that constitute the modification subunits of type I restriction-modification systems. The distal gene, bcgIB, codes for a 341-amino acid protein (molecular mass = 39.2 kDa) that resembles none of the sequences in the sequence data bases. The two genes overlap by several nucleotides. Alone, neither protein restricts or modifies DNA, but, together, they form a complex in the proportion A2B that does both. DNA binding assays showed that the DNA-protein complex can be formed only in the presence of both subunits, suggesting that the association of inactive subunits generates the active BcgI enzyme that can bind DNA and then either cleaves or methylates at target site.


Subject(s)
Bacillus/enzymology , Deoxyribonucleases, Type II Site-Specific/metabolism , Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism , Adenine/analogs & derivatives , Adenine/biosynthesis , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Bacterial/metabolism , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Electrophoresis, Agar Gel , Electrophoresis, Polyacrylamide Gel , Genes, Bacterial , Methylation , Molecular Sequence Data , Oligodeoxyribonucleotides , Protein Binding , Restriction Mapping , Sequence Homology, Amino Acid , Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics , Site-Specific DNA-Methyltransferase (Adenine-Specific)/isolation & purification
13.
J Bacteriol ; 173(16): 5207-19, 1991 Aug.
Article in English | MEDLINE | ID: mdl-1650347

ABSTRACT

The mrr gene of Escherichia coli K-12 is involved in the acceptance of foreign DNA which is modified. The introduction of plasmids carrying the HincII, HpaI, and TaqI R and M genes is severely restricted in E. coli strains that are Mrr+. A 2-kb EcoRI fragment from the plasmid pBg3 (B. Sain and N. E. Murray, Mol. Gen. Genet. 180:35-46, 1980) was cloned. The resulting plasmid restores Mrr function to mrr strains of E. coli. The boundaries of the mrr gene were determined from an analysis of subclones, and plasmids with a functional mrr gene produce a polypeptide of 33.5 kDa. The nucleotide sequence of the entire fragment was determined; in addition to mrr, it includes two open reading frames, one of which encodes part of the hsdR. By using Southern blot analysis, E. coli RR1 and HB101 were found to lack the region containing mrr. The acceptance of various cloned methylases in E. coli containing the cloned mrr gene was tested. Plasmid constructs containing the AccI, CviRI, HincII, Hinfl (HhaII), HpaI, NlaIII, PstI, and TaqI N6-adenine methylases and SssI and HhaI C5-cytosine methylases were found to be restricted. Plasmid constructs containing 16 other adenine methylases and 12 cytosine methylases were not restricted. No simple consensus sequence causing restriction has been determined. The Mrr protein has been overproduced, an antibody has been prepared, and the expression of mrr under various conditions has been examined. The use of mrr strains of E. coli is suggested for the cloning of N6-adenine and C5-cytosine methyl-containing DNA.


Subject(s)
DNA Restriction Enzymes/genetics , DNA-Cytosine Methylases/metabolism , Escherichia coli Proteins , Escherichia coli/genetics , Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism , Amino Acid Sequence , Base Sequence , Blotting, Southern , Blotting, Western , Cloning, Molecular , DNA Restriction Enzymes/biosynthesis , DNA Restriction Enzymes/chemistry , Endonucleases/genetics , Escherichia coli/physiology , Gene Expression/physiology , Molecular Sequence Data , Open Reading Frames/genetics
14.
Nucleic Acids Res ; 19(4): 841-50, 1991 Feb 25.
Article in English | MEDLINE | ID: mdl-1901989

ABSTRACT

The BamHI restriction modification system was previously cloned into E. coli and maintained with an extra copy of the methylase gene on a high copy vector (Brooks et al., (1989) Nucl. Acids Res. 17, 979-997). The nucleotide sequence of a 3014 bp region containing the endonuclease (R) and methylase (M) genes has now been determined. The sequence predicts a methylase protein of 423 amino acids, Mr 49,527, and an endonuclease protein of 213 amino acids, Mr 24,570. Between the two genes is a small open reading frame capable of encoding a 102 amino acid protein, Mr 13,351. The M. BamHI enzyme has been purified from a high expression clone, its amino terminal sequence determined, and the nature of its substrate modification studied. The BamHI methylase modifies the internal C within its recognition sequence at the N4 position. Comparisons of the deduced amino acid sequence of M. BamHI have been made with those available for other DNA methylases: among them, several contain five distinct regions, 12 to 22 amino acids in length, of pronounced sequence similarity. Finally, stability and expression of the BamHI system in both E. coli and B. subtilis have been studied. The results suggest R and M expression are carefully regulated in a 'natural' host like B. subtilis.


Subject(s)
DNA-Cytosine Methylases/genetics , Gene Expression Regulation, Bacterial , Gene Expression Regulation, Enzymologic , Amino Acid Sequence , Bacillus subtilis/genetics , Cloning, Molecular , Codon , DNA, Bacterial/genetics , DNA-Cytosine Methylases/isolation & purification , Escherichia coli/genetics , Molecular Sequence Data , Plasmids , Restriction Mapping , Sequence Homology, Nucleic Acid
15.
Gene ; 80(2): 193-208, 1989 Aug 15.
Article in English | MEDLINE | ID: mdl-2684765

ABSTRACT

The genes for FokI, a type-IIS restriction-modification system from Flavobacterium okeanokoites (asymmetric recognition sequence: 5'-GGATG/3'-CCTAC), were cloned into Escherichia coli. Recombinants carrying the fokIR and fokIM genes were found to modify their DNA completely, and to restrict lambdoid phages weakly. The nt sequences of the genes were determined, and the probable start codons were confirmed by aa sequencing. The FokI endonuclease (R.FokI) and methyltransferase (M.FokI) are encoded by single, adjacent genes, aligned in the same orientation, in the order M then R. The genes are large by the standards of type-II systems, 1.9 kb for the M gene, and 1.7 kb for the R gene. Preceding each gene is a pair of FokI recognition sites; it is conceivable that interactions between the sites and the FokI proteins could regulate expression of the genes. The aa sequences of the N- and C-terminal halves of M.FokI are similar to one another, and to certain other DNA-adenine methyltransferases, suggesting that the enzyme has a 'tandem' structure, such as could have arisen by the fusion of a pair of adjacent, ancestral M genes. Truncated derivatives of M. FokI were constructed by deleting the 5'- or 3'-ends of the fokIM gene. Deleting most of the C-terminus of M.FokI produced derivatives that methylated only the top (GGATG) strand of the recognition sequence. Conversely, deleting most of the N-terminus produced derivatives that methylated only the bottom (CATCC) strand of the recognition sequence. These results indicate that the domains in M.FokI for methylating the two strands of the recognition sequence are largely separate.


Subject(s)
Deoxyribonucleases, Type II Site-Specific/genetics , Flavobacterium/enzymology , Methyltransferases/genetics , Amino Acid Sequence , Base Sequence , Binding Sites , Cloning, Molecular , DNA Mutational Analysis , Escherichia coli/genetics , Flavobacterium/genetics , Molecular Sequence Data , Substrate Specificity
16.
Nucleic Acids Res ; 17(3): 979-97, 1989 Feb 11.
Article in English | MEDLINE | ID: mdl-2537955

ABSTRACT

BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Although within the clone the methylase and endonuclease genes are present on the same pACYC184 vector, the system can be maintained in E. coli only with an additional copy of the methylase gene present on a separate vector. The initial selection for BamHI methylase activity also yielded a second BamHI methylase gene which is not homologous in DNA sequence and hybridizes to different genomic restriction fragments than does the endonuclease-linked methylase gene. Finally, the interaction of the BamHI system with the E. coli Dam and the Mcr A and B functions, have been studied and are reported here.


Subject(s)
Bacillus/enzymology , Bacterial Proteins/genetics , Cloning, Molecular , Deoxyribonuclease BamHI/genetics , Amino Acid Sequence , Bacillus/genetics , Bacterial Proteins/isolation & purification , Base Sequence , Cloning, Molecular/methods , DNA Modification Methylases/genetics , DNA Probes/genetics , DNA Probes/isolation & purification , DNA Transposable Elements , DNA, Bacterial/isolation & purification , Deoxyribonuclease BamHI/isolation & purification , Deoxyribonuclease BamHI/physiology , Escherichia coli/genetics , Gene Rearrangement , Molecular Sequence Data , Plasmids , Restriction Mapping/methods , Transformation, Genetic
17.
Gene ; 74(1): 253-9, 1988 Dec 25.
Article in English | MEDLINE | ID: mdl-3248728

ABSTRACT

The sequences of the genes coding for M.CviBIII (from virus NC-1A which infects a eukaryotic alga) [Narva et al., Nucleic Acids Res. 15 (1987) 9807-9823] and M.TaqI (from the bacterium Thermus aquaticus) [Slatko et al., Nucleic Acids Res. 15 (1987) 9781-9796] have been determined recently. Both enzymes methylate adenine in the sequence TCGA. We have compared the predicted amino acid sequences of these two methyltransferases (MTases), with each other and with ten other N6 A-MTases and find regions of similarity. M.CviBIII and M.TaqI were most closely related followed by M.PaeR7, whose recognition sequence (CTCGAG) contains the M.TaqI/M.CviBIII recognition sequence TCGA, and M.PstI, whose recognition sequence is CTGCAG. All of the N6-MTases contain the sequence Asp/Asn-Pro-Pro-Tyr (B-P-P-Y) referred to by Hattman et al. [J. Bacteriol. 164 (1985) 932-937] as region IV. The predicted secondary structure of this region forms a finger-like structure ('beta finger') containing a beta-pleated sheet (...XXXB), two beta-turns (P-P) followed by another beta-pleated sheet [Y/FXXX...].


Subject(s)
Bacterial Proteins/genetics , Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics , Viral Proteins/genetics , Amino Acid Sequence , Chlorella , Molecular Sequence Data , Plant Viruses/enzymology , Plant Viruses/genetics , Protein Conformation , Sequence Homology, Nucleic Acid , Thermus/enzymology
20.
Nucleic Acids Res ; 15(23): 9781-96, 1987 Dec 10.
Article in English | MEDLINE | ID: mdl-2827113

ABSTRACT

The Taq I modification and restriction genes (recognition sequence TCGA) have been cloned in E. coli and their DNA sequences have been determined. Both proteins were characterized and the N-terminal sequence of the endonuclease was determined. The genes have the same transcriptional orientation with the methylase gene 5' to the endonuclease gene. The methylase gene is 1089 bp in length (363 amino acids, 40,576 daltons); the endonuclease gene is 702 bp in length (234 amino acids, 27,523 daltons); they are separated by 132 bp. Both methylase and endonuclease activity can be detected in cell extracts. The clones fully modify the vector and chromosomal DNA but they fail to restrict infecting phage. Clones carrying only the restriction gene are viable even in the absence of modification. The restriction gene contains 7 Taq I sites; the modification gene contains none. This asymmetric distribution of sites could be important in the regulation of the expression of the endonuclease gene.


Subject(s)
Cloning, Molecular , DNA Restriction Enzymes/genetics , DNA, Bacterial/genetics , Deoxyribonucleases, Type II Site-Specific , Genes, Bacterial , Amino Acid Sequence , Bacteriophage lambda/genetics , Base Sequence , Chromosome Mapping , Cloning, Molecular/methods , Codon , DNA Restriction Enzymes/isolation & purification , DNA, Bacterial/isolation & purification , Escherichia coli/enzymology , Escherichia coli/genetics , Molecular Sequence Data , Species Specificity , Thermus/enzymology , Thermus/genetics
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