New evidence for Cu-decorated binary-oxides mediating bacterial inactivation/mineralization in aerobic media.

Binary oxide semiconductors TiO2-ZrO2 and Cu-decorated TiO2-ZrO2 (TiO2-ZrO2-Cu) uniform films were sputtered on polyester (PES). These films were irradiated under low intensity solar simulated light and led to bacterial inactivation in aerobic and anaerobic media as evaluated by CFU-plate counting. But bacterial mineralization was only induced by TiO2-ZrO2-Cu in aerobic media. The highly oxidative radicals generated on the films surface under light were identified by the use of appropriate scavengers. The hole generated on the TiO2-ZrO2 films is shown to be the main specie leading to bacterial inactivation. TiO2-ZrO2 and Cu-decorated TiO2-ZrO2 films release Zr and Ti <1ppb and Cu 4.6ppb/cm(2) as determined by inductively coupled plasma mass spectrometry (ICP-MS) This level is far below the citotoxicity permitted level allowed for mammalian cells suggesting that bacterial disinfection proceeds through an oligodynamic effect. By Fourier transform attenuated infrared spectroscopy (ATR-FTIR) the systematic shift of the predominating νs(CH2) vibrational-rotational peak making up most of the bacterial cell-wall content in C was monitored. Based on this evidence a mechanism suggested leading to CH bond stretching followed by cell lysis and cell death. Bacterial inactivation cycling was observed on TiO2-ZrO2-Cu showing the stability of these films leading to bacterial inactivation.

Road runoff management using over-the-shoulder infiltration: real-scale experimentation.

A new management policy regarding road runoff was proposed in 2002 by the Swiss Federal Office for the Environment (FOEN). This new concept is based on the diffuse infiltration of road runoff into embankment slopes, where soils will filter particles and contaminants. The shoulder lying between road surface and infiltration slopes must be impervious in order to maximise the amount of water reaching the slope and avoid losses in the road structure. The implementation of this new concept should lower the impact of roads on the environment, improve aquifer recharge and reduce construction costs. The Swiss Federal Road Office (FedRO) decided to carry out real-scale investigations regarding this new policy and thus commissioned the GEOLEP to design, build, and test 5 different shoulder structures. This paper presents the results of a 2-years survey of infiltration processes in these shoulders to establish the best performing structure. The first three shoulders were overlaid with 5 cm of gravel mixed with humus, gravel mixed with clay, and seeded with lawn, respectively. The latter two had impervious layers located 26 cm deep: the road bituminous basement (road base) was prolonged and coated with bitumen in the first case, and a sodic-bentonite geotextile was used in the second. Both were covered with gravel. All shoulders were equipped with basal collecting devices that measured hydraulic fluxes seeping through the shoulders. In total, 112 natural precipitations and 3 artificial events were monitored. Artificial events mimicked known transitory regimes (thunderstorms) or were performed with constant regime. The goal was to effectively assess infiltration processes in shoulders. Results showed that shoulders made of gravel and humus or lawn were highly ineffective (only 30 to 40% of runoff is conducted to the infiltration slope). Gravel and clay was more efficient with a proportion of approximately 60%. The shoulder with prolonged road base showed similar results since the bituminous coating was in fact rather permeable. The best results were exhibited by the shoulder waterproofed with bentonitic geotextile, which allowed no water to penetrate. This material already proved to be very powerful in groundwater catchments. The authors thus proposed a combination of sodic-bentonite geotextile covered by a gravel and clay mixture. This would be the most efficient shoulder: it will convey all the runoff to infiltration slopes, thereby optimising its filtration, which in turn will enhance environmental conditions in the vicinity of roads.

The physiological and pathophysiological modulation of the endocrine function of the heart.

Under physiological conditions, the endocrine heart contributes to the maintenance of cardiovascular homeostasis through the polypeptide hormones ANF and BNP, which are members of the natriuretic peptide (NP) family. Given that NPs are of interest from the basic and clinical points of view, the genetic expression and secretion of ANF and BNP as well as the nature of the interaction of these hormones with their receptors has been the subject of extensive studies since the discovery of ANF in 1980. Following hemodynamic overload, increased secretion of NPs by the heart can be seen. This change may occur without an increase in gene expression as observed for atrial NPs following acute volume expansion, or it can occur with an increase in both ANF and BNP gene expression in atria only as seen in mineralocorticoid escape during which it is obvious that a critical decrease in hormone stores must be reached before transcriptional activation occurs. Chronic hemodynamic pressure or volume overload results in increased expression of NPs in atria and ventricles. Under these circumstances, the increased production of BNP by hypertrophic ventricles changes the normal plasma concentration ratio of ANF to BNP, a fact that has clinical diagnostic and prognostic implications. There are exceptions to this rule: chronic, severe L-NAME hypertension, which may occur without left ventricular hypertrophy, does not cause this effect and increased ventricular NP gene expression can occur in mineralocorticoid hypertension before detectable ventricular hypertrophy. Atrial and ventricular NP gene expression appears to be under different transcriptional control because pharmacological treatments such as chronic ACE inhibition or ET(A) receptor blockade can reverse the increased ventricular NP expression but has no detectable effect on atrial NP gene expression. This is not unlike the myosin heavy chain switch that is observed in certain pathologies, and can be pharmacologically reversed in a manner similar to NPs in the ventricles but it does not occur in atrial muscle. These observations made in vivo or using isolated adult atria often differ strikingly from results obtained using the mixed phenotype afforded by cardiocytes in culture, indicating that the kinds of questions addressed by each approach must be judiciously chosen. G-protein coupled receptor-mediated actions of neurohumors such as endothelin and phenylephrine are normally used to stimulate NP gene expression and release in different in vitro models. The main physiological stimulus for increased ANF release, atrial muscle stretch, also appears to rely on G-protein-coupled mechanisms. Alternative agonists and receptor types at play are suggested by the finding that circulating levels of BNP are selectively increased before and during overt cardiac allograft rejection episodes in human patients. The data suggest that enhanced BNP plasma levels could form a basis for a noninvasive test for cardiac allograft rejection. However, the molecular mechanism by which expression of NPs are regulated in the transplanted heart is not well understood. Conditioned medium from mixed lymphocyte reaction cultures, considered an in vitro model of transplantation immunity, induces specific upregulation of BNP as do individual pro-inflammatory cytokines. Findings such as these suggest that the study of NPs will continue to produce a wealth of information relevant to basic and clinical scientists.

[Ultrasonic assessment of thyroid nodules, and iodine status of French adults participating in the SU.VI.MAX study]. / Nodules thyroïdiens à l'échographie et statut en iode des adultes volontaires de l'étude SU.VI.MAX.

Thyroid nodules were assessed by ultrasound in 3 621 presumably healthy free-living French subjects participating in the SU.VI.MAX cohort (2 160 females aged 35-60 years and 1 461 males aged 45-60 years). Urinary iodine was measured in all participants in random morning urine samples. Nodular thyroid structures ranging from 3 mm to 57 mm in diameter were found in 14.5% of the population, without significant differences between regions in the different age and sex groups. Single nodules and multiple lesions were found in, respectively, 9.7% and 3.1% of the subjects. In females, nodules increased from 12.9% to 19.2% between 35-45 years and 45-60 years, respectively (p<0.001). Thyroid nodularity was more frequent among women aged 45-60 years than among men of those ages (19.2% vs. 11.0%, p=0.001). Descriptive data provided by ultrasound differentiated the nodules into solid (43.4%), cystic (38.4%), and mixed solid-cystic (18.2%). Most solid thyroid nodules, 76.1%, were hypoechoic, while 16.6% were iso-, and 7.3% were hyperechoic. The median of iodine concentrations (n=3 276) was 8.0 microgram/100 ml, with 19.4% of the samples being<5 microgram/100 ml. Median iodine concentrations displayed wide regional variations, with values significantly higher in residents of western regions than among those living in the eastern areas (p<0.001), independently of age and sex. No relationship was found between prevalence of nodular thyroid structure and the state of borderline iodine status observed in France.

Determinants of thyroid volume in healthy French adults participating in the SU.VI.MAX cohort.

OBJECTIVE: To study the relative importance of determinants of thyroid volume. DESIGN: Cross-sectional study on a sample of subjects issued from the SU.VI.MAX cohort. SUBJECTS: 2987 French subjects (1713 women aged 35-60 years and 1274 men aged 45-60 years). None of them had previous or present thyroid disease. MEASUREMENTS: Thyroid volume was determined by ultrasound. Serum TSH and free thyroxine (fT4) were measured in duplicate. Urinary iodine and urinary thiocyanate were assayed in random morning urine samples. RESULTS: For both sexes, thyroid volume (ml) was positively correlated with weight, height, body mass index and body surface area (P = 0.0001) and negatively with age for females (P = 0.0009). When the urinary iodine concentration was adjusted for urinary thiocyanate concentration and their interaction, the thyroid volume was negatively correlated with urinary iodine (males P = 0.02, females P = 0.006) and positively correlated with urinary thiocyanate (males P = 0.0001, females P = 0.004). Mean thyroid volume was greater among active smokers than non-smokers (males P < 0.0001, females P = 0.0004) and was greater among former smokers than among non-smokers (males P = 0.0001, females = 0.004). Free T4 and thyroid volume were positively correlated for both sexes (P = 0. 0001). TSH was negatively correlated with thyroid volume for both groups (P = 0.0001). Female users of oral contraception (aged 35-45 years) had a smaller thyroid volume than non-users (P = 0.0009). CONCLUSIONS: The state of borderline iodine deficiency observed in France, in association with a slightly goitrogenic environment, may result in sustained stimulation of the thyroid, independently of TSH level, and is of paramount importance in the formation of goitre. Smoking may affect the thyroid, inducing marked long-lasting thyroid enlargement.

Late operating room starts: experience with an education trial.

PURPOSE: This study was undertaken to determine if late starts of first cases in the Operating theatres at the SMBD-Jewish General Hospital remained a problem after identification of the causes of late starts and remedial actions being taken. METHODS: Hospital approval was obtained. A retrospective chart audit analyzed a two week period (10 days with 90 elective surgical cases) in October 1993. The time of entry by the first patient into each Operating Room (OR) was transcribed from the nursing records from each OR. A late start was defined as patient entry into the OR after 0745 hr. This audit revealed 77.8% of patients scheduled' for surgery at 0745 entered the OR late with a cumulative time lost of 1101 min. The reasons for this inefficiency were identified by a follow-up assessment in April 1995 as a result of this audit. Corrective measures included presentation of inpatients for the first case, reorganization of transport personnel schedules to facilitate arrival of patients to the OR, alteration of patient verification procedures prior to entry to the OR, and education of nursing, anaesthesia, and surgical personnel of the scope of the problem of late OR starts. All attending surgeons were notified either by letter or by discussion at departmental rounds. These measures were in effect by July 1995. A second audit, using the same methodology as the first, evaluated a two week period (10 days with 87 elective surgical cases) in October 1995. RESULTS: The second audit showed 65.5% of patients (average of 9 operating rooms daily) scheduled for surgery at 0745 entered the OR late with 601 min lost. The average delay for late starting cases decreased from 15.73 +/- 4.56 to 10.54 +/- 3.92 min (P < 0.05). CONCLUSION: Late OR starts are common and only modest improvements can be achieved without cooperation from anaesthetists and surgeons to arrive on time.