ABSTRACT
Cells defective in any of the RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3) are sensitive to DNA cross-linking agents and to ionizing radiation. Because the paralogs are required for the assembly of DNA damage-induced RAD51 foci, and mutant cell lines are defective in homologous recombination and show genomic instability, their defect is thought to be caused by an inability to promote efficient recombinational repair. Here, we show that the five paralogs exist in two distinct complexes in human cells: one contains RAD51B, RAD51C, RAD51D, and XRCC2 (defined as BCDX2), whereas the other consists of RAD51C with XRCC3. Both protein complexes have been purified to homogeneity and their biochemical properties investigated. BCDX2 binds single-stranded DNA and single-stranded gaps in duplex DNA, in accord with the proposal that the paralogs play an early (pre-RAD51) role in recombinational repair. Moreover, BCDX2 complex binds specifically to nicks in duplex DNA. We suggest that the extreme sensitivity of paralog-defective cell lines to cross-linking agents is owing to defects in the processing of incised cross links and the consequential failure to initiate recombinational repair at these sites.
Subject(s)
DNA Repair/physiology , DNA-Binding Proteins/isolation & purification , Testis/chemistry , Adenosine Triphosphatases/metabolism , Baculoviridae/genetics , Chromatography, Gel , DNA Repair/genetics , DNA, Single-Stranded/metabolism , DNA-Binding Proteins/metabolism , Humans , Male , Microscopy, Electron , Precipitin Tests , Protein Binding , Protein Isoforms/isolation & purification , Protein Isoforms/metabolism , Rad51 Recombinase , Recombinant Proteins/metabolism , Recombination, Genetic , Testis/cytologyABSTRACT
In vertebrates, the RAD51 protein is required for genetic recombination, DNA repair, and cellular proliferation. Five paralogs of RAD51, known as RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3, have been identified and also shown to be required for recombination and genome stability. At the present time, however, very little is known about their biochemical properties or precise biological functions. As a first step toward understanding the roles of the RAD51 paralogs in recombination, the human RAD51C and XRCC3 proteins were overexpressed and purified from baculovirus-infected insect cells. The two proteins copurify as a complex, a property that reflects their endogenous association observed in HeLa cells. Purified RAD51C--XRCC3 complex binds single-stranded, but not duplex DNA, to form protein--DNA networks that have been visualized by electron microscopy.
Subject(s)
DNA Repair , DNA, Single-Stranded/metabolism , DNA-Binding Proteins/metabolism , Animals , Cell Line , DNA-Binding Proteins/isolation & purification , HeLa Cells , Humans , Mice , Microscopy, Electron , Oligodeoxyribonucleotides/metabolism , Rabbits , Rad51 Recombinase , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Recombination, Genetic , SpodopteraABSTRACT
The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.
Subject(s)
Adenosine Triphosphatases/genetics , Adenosine Triphosphatases/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Genes, Protozoan , Leishmania major/genetics , Adenosine Triphosphatases/chemistry , Amino Acid Sequence , Animals , Cloning, Molecular , DNA, Protozoan/metabolism , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/isolation & purification , Humans , Leishmania major/drug effects , Leishmania major/growth & development , Leishmania major/metabolism , Molecular Sequence Data , Phleomycins/pharmacology , Rad51 Recombinase , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sequence Analysis, DNAABSTRACT
Eukaryotic cells encode two homologs of Escherichia coli RecA protein, Rad51 and Dmc1, which are required for meiotic recombination. Rad51, like E.coli RecA, forms helical nucleoprotein filaments that promote joint molecule and heteroduplex DNA formation. Electron microscopy reveals that the human meiosis-specific recombinase Dmc1 forms ring structures that bind single-stranded (ss) and double-stranded (ds) DNA. The protein binds preferentially to ssDNA tails and gaps in duplex DNA. hDmc1-ssDNA complexes exhibit an irregular, often compacted structure, and promote strand-transfer reactions with homologous duplex DNA. hDmc1 binds duplex DNA with reduced affinity to form nucleoprotein complexes. In contrast to helical RecA/Rad51 filaments, however, Dmc1 filaments are composed of a linear array of stacked protein rings. Consistent with the requirement for two recombinases in meiotic recombination, hDmc1 interacts directly with hRad51.
Subject(s)
Adenosine Triphosphatases/metabolism , Adenosine Triphosphatases/ultrastructure , Cell Cycle Proteins , DNA Nucleotidyltransferases/metabolism , DNA Nucleotidyltransferases/ultrastructure , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/ultrastructure , Integrases , Adenosine Triphosphatases/isolation & purification , Cloning, Molecular , DNA Nucleotidyltransferases/isolation & purification , DNA, Single-Stranded/biosynthesis , DNA, Single-Stranded/chemistry , DNA, Viral/biosynthesis , DNA, Viral/chemistry , DNA-Binding Proteins/isolation & purification , Escherichia coli/genetics , Gene Library , Humans , Male , Meiosis , Microscopy, Electron , Nucleic Acid Heteroduplexes/biosynthesis , Nucleic Acid Heteroduplexes/chemistry , Organ Specificity , Rad51 Recombinase , Rec A Recombinases/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Recombinant Proteins/ultrastructure , Recombinases , Recombination, Genetic , Testis/enzymologyABSTRACT
Error-free repair by homologous recombination of DNA double-strand breaks induced by ionizing radiation (IR) requires the Rad52 group proteins, including Rad51 and Rad54, in the yeast Saccharomyces cerevisiae [1]. The formation of a 'joint' molecule between the damaged DNA and the homologous repair template is a key step in recombination mediated by Rad51 and stimulated by Rad54 [2] [3] [4] [5]. Mammalian homologs of Rad51 and Rad54 have been identified [2] [3] [6]. Here, we demonstrate that mouse Rad54 (mRad54) formed IR-induced nuclear foci that colocalized with mRad51. Interaction between mRad51 and mRad54 was induced by genotoxic stress, but only when lesions that required mRad54 for their repair were formed. Interestingly, mRad54 was essential for the formation of IR-induced mRad51 foci. Rad54 belongs to the SWI2/SNF2 protein family, members of which modulate protein-DNA interactions in an ATP-driven manner [7]. Results of a topological assay suggested that purified human Rad54 (hRad54) protein can unwind double-stranded (ds) DNA at the expense of ATP hydrolysis. Unwinding of the homologous repair template could promote the formation or stabilization of hRad51-mediated joint molecules. Rad54 appears to be required downstream of other Rad52 group proteins, such as Rad52 and the Rad55-Rad57 heterodimer, that assist Rad51 in interacting with the broken DNA [2] [3] [4].
Subject(s)
DNA Damage , DNA Repair/physiology , DNA-Binding Proteins/physiology , DNA/radiation effects , Fungal Proteins/physiology , Nucleic Acid Conformation , Saccharomyces cerevisiae Proteins , Adenosine Triphosphate/physiology , Animals , Cell Line , DNA/metabolism , DNA Helicases , DNA Repair Enzymes , DNA, Complementary/genetics , Electrophoresis, Gel, Two-Dimensional , Exons/genetics , Gene Targeting , Genes, Reporter , Hemagglutinins/genetics , Humans , Mice , Microscopy, Fluorescence , Multigene Family , Promoter Regions, Genetic , Rad51 Recombinase , Recombination, Genetic/physiology , Stem Cells/radiation effects , Templates, GeneticABSTRACT
In the yeast Saccharomyces cerevisiae, mutations in the genes RAD51 or RAD52 result in severe defects in genetic recombination and the repair of double-strand DNA breaks. These genes, and others of the RAD52 epistasis group (RAD50, RAD54, RAD55, RAD57, RAD59, MRE11 and XRS2), were first identified by their sensitivity to X-rays. They were subsequently shown to be required for spontaneous and induced mitotic recombination, meiotic recombination, and mating-type switching. Human homologues of RAD50, RAD51, RAD52, RAD54 and MRE11 have been identified. Targeted disruption of the murine RAD51 gene results in an embryonic lethal phenotype, indicating that Rad51 protein is required during cell proliferation. Biochemical studies have shown that human RAD51 encodes a protein of relative molecular mass 36,966 (hRad51) that promotes ATP-dependent homologous pairing and DNA strand exchange. As a structural and functional homologue of the RecA protein from Escherichia coli, hRad51 is thought to play a central role in recombination. Yeast Rad51 has been shown to interact with Rad52 protein, as does the human homologue. Here we show that hRad52 stimulates homologous pairing by hRad51. The DNA-binding properties of hRad52 indicate that Rad52 is involved in an early stage of Rad51-mediated recombination.
Subject(s)
DNA Repair , DNA-Binding Proteins/physiology , Recombination, Genetic , Cloning, Molecular , DNA/metabolism , DNA, Single-Stranded/metabolism , Electrophoresis, Agar Gel , Escherichia coli , Humans , Protein Binding , Rad51 Recombinase , Rad52 DNA Repair and Recombination Protein , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae ProteinsABSTRACT
In vitro, the human Rad51 protein (hRad51) promotes homologous pairing and strand exchange reactions suggestive of a key role in genetic recombination. To analyse its role in this process, polyclonal antibodies raised against hRad51 were used to study the distribution of Rad51 in human and mouse spermatocytes during meiosis I. In human spermatocytes, hRad51 was found to form discrete nuclear foci from early zygotene to late pachytene. The foci always co-localized with lateral element proteins, components of the synaptonemal complex (SC). During zygotene, the largest foci were present in regions undergoing synapsis, suggesting that Rad51 is a component of early recombination nodules. Pachytene nuclei showed a greatly reduced level of Rad51 labelling, with the exceptions of any asynapsed autosomes and XY segments, which were intensely labelled. The distribution of Rad51 in mouse spermatocytes was similar to that found in human spermatocytes, except that in this case Rad51 was detectable at leptotene. From these results, we conclude that the Rad51 protein has a role in the interhomologue interactions that occur during meiotic recombination. These interactions are spatially and temporally associated with synapsis during meiotic prophase I.
Subject(s)
DNA Nucleotidyltransferases/isolation & purification , DNA-Binding Proteins/isolation & purification , Integrases , Meiosis , Recombination, Genetic , Spermatocytes/ultrastructure , Animals , Humans , Immunohistochemistry , Male , Mice , Rad51 Recombinase , Recombinases , Species Specificity , Synaptonemal ComplexABSTRACT
The human Rad51 protein is a structural homolog of Escherichia coli RecA. The exact role of human Rad51 within the cell is poorly understood but, like its bacterial and yeast homologs, hRad51 is believed to play a central role in homologous recombination. However, recent reports that transgenic mice lacking the RAD51 gene die early in development suggest an additional and essential function for mammalian Rad51 in cell proliferation or genome maintenance. In this paper we describe a simple and quick method for the purification of human Rad51 overproduced in E. coli. Dialysis of cell-free extracts against buffer containing low concentrations of spermidine result in the formation of hRad51 microcrystals as observed by light and electron microscopy. The crystals were easily redissolved in phosphate buffer and hRad51 was further purified to homogeneity using hydroxylapatite, affi-gel heparin and Q-sepharose chromatography. When purified by this method hRad51 is free of endo- and exonuclease activities and suitable for enzymological studies. Spermidine precipitation also provides a rapid method for the large scale purification of hRad51 suitable for physical analysis.
Subject(s)
DNA-Binding Proteins/isolation & purification , Recombinant Proteins/isolation & purification , Spermidine/chemistry , Chemical Precipitation , Chromatography, Liquid/methods , Crystallization , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Escherichia coli/genetics , Humans , Microscopy, Electron , Rad51 Recombinase , Recombinant Proteins/genetics , Recombinant Proteins/metabolismABSTRACT
The human testis Rad51 protein, a structural homolog of E. coli RecA, binds single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. Using circular ssDNA and linear dsDNA (3.0 kb in length), we demonstrate that hRad51 promotes homologous pairing and strand exchange reactions in vitro. Joint molecule formation was dependent upon ATP hydrolysis and DNA homology and was stimulated by the single-strand DNA-binding protein RP-A. In these reactions, the 5' terminus of the complementary strand of the linear duplex was efficiently transferred to the ssDNA. However, under standard conditions, extensive strand exchange was not observed. These results establish hRad51 as a functional homolog of RecA, but indicate that the human protein and its bacterial counterpart differ in their ability to promote extensive strand transfer. It is proposed that hRad51 mediates homology recognition and initiates strand exchange, but that extensive heteroduplex formation in higher organisms requires the actions of additional proteins.
Subject(s)
Adenosine Triphosphate/metabolism , DNA-Binding Proteins/metabolism , DNA/metabolism , Recombination, Genetic , Chemical Precipitation , DNA Ligases/metabolism , DNA, Circular , DNA, Single-Stranded/metabolism , Humans , Male , Nucleic Acid Conformation , Nucleoproteins/metabolism , Protein Binding , Rad51 Recombinase , Rec A Recombinases/genetics , Sequence Homology, Nucleic Acid , Species Specificity , Spermidine , Testis/chemistryABSTRACT
In bacteria, genetic recombination is catalysed by RecA protein, the product of the recA gene. A human gene that shares homology with Escherichia coli recA (and its yeast homologue RAD51) has been cloned from a testis cDNA library, and its 37 kDa product (hRad51) purified to homogeneity. The human Rad51 protein binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. Using a topological assay, we demonstrate that hRad51 underwinds duplex DNA, in a reaction dependent upon the presence of ATP or its non-hydrolysable analogue ATP gamma S. Complexes formed with single- and double-stranded DNA have been observed by electron microscopy following negative staining. With nicked duplex DNA, hRad51 forms helical nucleoprotein filaments which exhibit the striated appearance characteristic of RecA or yeast Rad51 filaments. Contour length measurements indicate that the DNA is underwound and extended within the nucleoprotein complex. In contrast to yeast Rad51 protein, human Rad51 forms filaments with single-stranded DNA in the presence of ATP/ATP gamma S. These resemble the inactive form of the RecA filament which is observed in the absence of a nucleotide cofactor.
Subject(s)
DNA-Binding Proteins/isolation & purification , Escherichia coli/metabolism , Rec A Recombinases/metabolism , Adenosine Triphosphatases/metabolism , Adenosine Triphosphate/metabolism , Cloning, Molecular , DNA Topoisomerases, Type I/metabolism , DNA, Complementary , DNA, Superhelical/chemistry , DNA, Superhelical/metabolism , DNA, Superhelical/ultrastructure , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/ultrastructure , Escherichia coli/genetics , Gene Library , Humans , Male , Rad51 Recombinase , Rec A Recombinases/ultrastructure , Testis/metabolismABSTRACT
The specificity of the Escherichia coli RuvC Holliday junction resolvase has been investigated in vitro. RuvC protein cleaves synthetic DNA substrates that model three- or four-stranded recombination intermediates but fails to act upon Y junctions, G/A mismatches, heterologous loop structures, or two-stranded branched junctions. RuvC therefore differs from endonuclease VII of bacteriophage T4 which exhibits broad range specificity. Using related three- and four-stranded synthetic DNA junctions, we show that RuvC cleaves both junctions at the same DNA sequence and requires a region of homology at the junction point. The action of RuvC on three- and four-stranded recombination intermediates made by RecA was also investigated. We found that RuvC fails to resolve three-stranded intermediates in the presence of RecA, although four-stranded intermediates are resolved under the same conditions. However, both three- and four-stranded intermediates are substrates for the nuclease after removal of RecA. We interpret these differences in terms of the contiguity of the RecA nucleoprotein filament which may, under certain conditions, limit access to the Holliday junction resolvase.
Subject(s)
Bacterial Proteins/metabolism , DNA/metabolism , Endodeoxyribonucleases , Escherichia coli Proteins , Escherichia coli/metabolism , Nucleotidyltransferases/metabolism , Oligodeoxyribonucleotides/metabolism , Bacterial Proteins/isolation & purification , Base Sequence , DNA/chemical synthesis , DNA/chemistry , Escherichia coli/enzymology , Macromolecular Substances , Models, Structural , Molecular Sequence Data , Nucleic Acid Conformation , Oligodeoxyribonucleotides/chemical synthesis , Rec A Recombinases/isolation & purification , Rec A Recombinases/metabolism , Substrate Specificity , TransposasesABSTRACT
A mammalian endonuclease that resolves Holliday junctions has been partially purified from extracts of calf thymus and Chinese hamster ovary cells. The activity acts upon (i) synthetic Holliday junctions and (ii) recombination intermediates made by the Escherichia coli RecA protein and appears to be functionally analogous to the E. coli RuvC protein. Cleavage occurs by the introduction of symmetrically related nicks in strands of like polarity to produce nicked duplex DNA products. The nicks can be repaired by DNA ligase. The resolvase is specific for Holliday junctions and does not act upon Y junctions, G/A mismatches, or heterologous loops. The substrate specificity is therefore similar to that of E. coli RuvC protein and contrasts with the broad range specificity of other junction resolvases such as T4 endonuclease VII. The mammalian resolvase activity has been observed at normal levels in extracts prepared from a series of DNA repair-defective cells. These include the x-ray or UV-sensitive hamster lines xrs-5, xrs-6, and Chinese hamster ovary 43-3B (defective in ERCC-1), and murine cells that are severely immunodeficient and defective in both V(D)J rejoining and DNA repair.
Subject(s)
Bacterial Proteins/metabolism , Endodeoxyribonucleases , Escherichia coli Proteins , Escherichia coli/genetics , Escherichia coli/metabolism , Nucleotidyltransferases/metabolism , Oligodeoxyribonucleotides/metabolism , Recombination, Genetic , Thymus Gland/metabolism , Animals , Base Sequence , CHO Cells , Cattle , Chromatography, Affinity , Chromatography, Ion Exchange , Cricetinae , DNA, Viral/isolation & purification , DNA, Viral/metabolism , Escherichia coli/enzymology , Molecular Sequence Data , Nucleotidyltransferases/isolation & purification , Oligodeoxyribonucleotides/chemical synthesis , Oligodeoxyribonucleotides/chemistry , Substrate Specificity , TransposasesSubject(s)
Escherichia coli Proteins , Escherichia coli/genetics , Recombination, Genetic , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , DNA Damage , DNA Helicases/genetics , DNA Helicases/metabolism , DNA Repair , DNA Replication , DNA, Bacterial/genetics , DNA, Bacterial/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Endodeoxyribonucleases/genetics , Endodeoxyribonucleases/metabolism , Escherichia coli/metabolism , Genes, Bacterial , Kinetics , Models, GeneticABSTRACT
The recombination of DNA molecules has been reconstituted in vitro using two purified enzymes from Escherichia coli. RecA protein catalyses homologous pairing and strand exchange reactions to form intermediate DNA structures that are acted upon by RuvC. The newly identified RuvC protein resolves the intermediates by specific endonucleolytic cleavage to produce recombinant DNA molecules.
Subject(s)
Bacterial Proteins/metabolism , Endodeoxyribonucleases , Escherichia coli Proteins , Escherichia coli/genetics , Rec A Recombinases/metabolism , Recombination, Genetic , Bacterial Proteins/genetics , Bacterial Proteins/isolation & purification , Base Sequence , Binding Sites , Cloning, Molecular , DNA, Bacterial/genetics , Electrophoresis, Polyacrylamide Gel , Escherichia coli/metabolism , Genes, Bacterial , Macromolecular Substances , Models, Genetic , Molecular Sequence Data , Molecular Weight , Oligodeoxyribonucleotides , Plasmids , Rec A Recombinases/isolation & purification , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Sequence Homology, Nucleic AcidABSTRACT
In previous studies, Holliday junctions generated during RecA-mediated strand-exchange reactions were resolved by fractionated Escherichia coli extracts. We now report the specific binding and cleavage of synthetic Holliday junctions (50 base pairs long) by a fraction purified by chromatography on DEAE-cellulose, phosphocellulose, and single-stranded DNA-cellulose. The cleavage reaction provided a sensitive assay with which to screen extracts prepared from recombination/repair-deficient mutants. Cells with mutations in ruvC lack the nuclease activity that cleaves synthetic Holliday junctions in vitro. This deficiency was restored by a multicopy plasmid carrying a ruvC+ gene that overexpressed junction-resolving activity. The UV sensitivity and deficiency in recombinational repair of DNA exhibited by ruv mutants lead us to suggest that RuvC resolves Holliday junctions in vivo.
Subject(s)
Bacterial Proteins/genetics , DNA Replication , DNA, Bacterial/genetics , Endodeoxyribonucleases , Escherichia coli Proteins , Escherichia coli/genetics , Genes, Bacterial , Bacteriophage phi X 174/genetics , Base Sequence , DNA, Viral/genetics , Gene Expression , Molecular Sequence Data , Oligonucleotide Probes , PlasmidsABSTRACT
Recombinant plasmids carrying ruvA, ruvB, or both were constructed and used to investigate the genetic defects in a collection of UV-sensitive ruv mutants. The results revealed that efficient survival of UV-irradiated cells depends on both ruvA and ruvB, and on a third gene, ruvC, located upstream of the ruvAB operon. Southern blotting analysis was used to locate insertions in ruv and to examine putative deletion mutants. Two Tn10 insertions were located to the region encoding ruvA. Since these insertions caused a deficiency in the activities of both ruvA and ruvB, we concluded that they must exert a polar effect on ruvB. Two putative ruv deletion mutants were shown to be the result of deletion-inversion events mediated during imprecise excision of Tn10. The relevant inversion breakpoints in these mutants were located to ruvA and ruvC.
Subject(s)
DNA Repair , Escherichia coli/genetics , Recombination, Genetic , Blotting, Southern , DNA Transposable Elements , DNA, Bacterial , Escherichia coli/radiation effects , Gene Expression Regulation, Bacterial , Genes, Bacterial , Genetic Complementation Test , Mutation , Plasmids , Recombination, Genetic/genetics , Restriction Mapping , Ultraviolet RaysABSTRACT
The nucleotide sequence of a 2505 bp region of the Escherichia coli chromosome containing the LexA regulated ruv gene has been determined. A sequence of 1631 bp encoding two non-overlapping open reading frames that constitute a single operon and which specify polypeptides with predicted molecular weights of 22172 daltons and 37177 daltons respectively, was identified as the most probable sequence for ruv. Each of the two open reading frames, designated ruvA and ruvB, is preceded by a reasonable Shine-Dalgarno sequence. Two 16 bp sequences (SOS boxes) that match the consensus sequence for binding LexA protein are located 5' to ruvA in a region that provides a possible single promoter for expression of both ruvA and ruvB, with the second SOS box overlapping the putative -35 region. A possible transcriptional terminator is located 137 bp downstream of ruvB. The amino acid sequence predicted for RuvB contains a region that matches a highly conserved sequence found in several DNA repair and recombination proteins that bind ATP.
Subject(s)
Bacterial Proteins/physiology , Escherichia coli/genetics , Genes, Bacterial , Genes, Regulator , Genes , Operon , Repressor Proteins/physiology , Serine Endopeptidases , Transcription Factors/physiology , Amino Acid Sequence , Base Sequence , Molecular Sequence Data , PlasmidsABSTRACT
Conjugational recombination in Escherichia coli was investigated by comparing the effects of recN, recO, ruv and lexA mutations on the formation of recombinants in crosses with strains lacking RecBCD enzyme. The results presented reveal that recN and ruv mutations do not abolish residual recombination in a recB mutant, and have only a rather modest effect on recombination in recBC sbcA strains; in these respects they are quite different from recF, recJ and recO mutations. The differences between these two groups of genes are discussed in relation to the molecular exchanges needed to produce viable recombinants.
Subject(s)
Conjugation, Genetic , Escherichia coli Proteins , Escherichia coli/genetics , Exodeoxyribonucleases/genetics , Recombination, Genetic , Escherichia coli/enzymology , Exodeoxyribonuclease V , MutationABSTRACT
The ruv gene of Escherichia coli, which is associated with inducible mechanisms of DNA repair and recombination, has been cloned into the low-copy plasmid vector pHSG415. The recombinant plasmid pPVA101 fully complements the DNA repair-deficient phenotype of ruv mutants. Restriction endonuclease analysis of this plasmid revealed a 10.6-kilobase (kb) HindIII DNA insert which contained a 7.7-kb PstI fragment identified as being from the chromosomal ruv region. Deletion analysis and Tn1000 insertional inactivation of ruv function located the ruv coding region to a 2.2-kb section of the cloned DNA fragment. A comparison of the proteins encoded by ruv wild-type and mutant plasmids identified the gene product as a protein of molecular weight 41,000.
Subject(s)
Bacterial Proteins/analysis , Chromosome Mapping , DNA Repair , Escherichia coli/genetics , Chromosome Deletion , DNA Transposable Elements , Mutation , Ultraviolet RaysABSTRACT
Mutation of the ruv gene of E. coli is associated with sensitivity to radiation, and filamentous growth after transient inhibition of DNA synthesis. The filamentation of ruv strains is abolished by mutations in sfiA or sfiB that prevent SOS induced inhibition of cell division, but this does not restore resistance to UV radiation. Double mutants carrying both ruv and uvr mutations are considerably more sensitive to UV radiation than the single mutants, but there is no additive effect of ruv with recA, recF, recB, or recC mutations. ruv mutations have little effect on conjugal recombination in wild-type strains but confer recombination deficiency and extreme sensitivity to ionizing radiation in recBC sbcB strains. These results, together with the fact that ruv strains are excision proficient and mutable by UV light, are interpreted to suggest that the ruv + product is involved in recombinational repair of damaged DNA rather than in cell division as suggested by Otsuji et al. (1974).