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1.
Rev Soc Bras Med Trop ; 28(2): 113-6, 1995.
Article in Portuguese | MEDLINE | ID: mdl-7716322

ABSTRACT

The biological cycle of Rhodnius nasutus was studied from november 1985 to September 1986 under laboratory conditions. The temperature ranged from 28.0 +/- 2 degrees C to 22.0 +/- 2 degrees C and 59.2 to 70.4% of humidity. Chickens were used as a blood meal source. The complete biological cycle was achieved with an average of 209.4 days of the 16 remaining insects from initial colony of the 30 Katomines. The average incubation period lasted 11.1 days and those for the following nymphal stages were: 17.6 days for the first; 16.1 days for the second; 30.1 days for the third; 71.2 days for the fourth and 79.9 days for the fifth nymphal stage.


Subject(s)
Rhodnius/growth & development , Animals , Humidity , Nymph/growth & development , Ovum/growth & development , Temperature , Time Factors
2.
Rev Soc Bras Med Trop ; 25(1): 51-8, 1992.
Article in Portuguese | MEDLINE | ID: mdl-1308066

ABSTRACT

In the rural areas of Teresina, 129 triatomines were captured distributed in (a) artificial ecotopes; a house with one Triatoma brasiliensis, one Panstrongylus geniculatus, Rhodnius pictipes, and one Rhodnius prolixus and in a uninhabited chicken house (7 Rhodnius nasutus). (b) Natural ecotopes; Pahus Orbignya martiana (41 Rhodnius neglectus, 33 Rhodnius prolixus and 41 Rhodnius nasutus) and Copernicia cerifera (3 Rhodnius neglectus). The 22.6% of captured triatomines were infected by flagellates similar to Trypanosoma cruzi. Twenty-eight sylvatic mammals were captured and examined. Seven Didelphia albiventris, 2 Rattus rattus and a Tamandua tetradactyla were infected with flagellates. The flagellates found in both triatomines and mammals were morphologically indistinguishable from Trypanosoma cruzi. Serology by the indirect immunofluorescence test for Chagas disease revealed two positive seroreactions of positivity among 123 inhabitants examined.


Subject(s)
Chagas Disease/epidemiology , Rural Population , Adolescent , Adult , Aged , Aged, 80 and over , Animals , Animals, Domestic/parasitology , Animals, Wild/parasitology , Brazil/epidemiology , Chagas Disease/parasitology , Chagas Disease/transmission , Child , Child, Preschool , Female , Humans , Infant , Insect Vectors/parasitology , Male , Middle Aged , Rats , Rural Population/statistics & numerical data , Triatoma/parasitology , Trypanosoma cruzi/isolation & purification
3.
Rev. Inst. Med. Trop. Säo Paulo ; 33(3): 193-8, maio-jun. 1991. tab
Article in English | LILACS | ID: lil-108380

ABSTRACT

A prevalencia da infeccao pelo Trypanosoma cruzi foi pesquisada em Oitis, uma pequena comunidade do Estado do Piaui. Duzentos e sessenta e cinco individuos foram investigados pelos seguintes metodos: pesquisa direta, hemocultura, imunofluorescencia indireta (IFA), teste imunoabsorvente ligado a enzima (ELISA), e ELISA de competicao (C-ELISA) com a utilizacao do anticorpo monoclonal TCF87 contra um antigeno do T.cruzi com 25kd. A IFA foi positiva em 14,3 por cento dos individuos, ELISA em 14,7 por cento e C-ELISA em 13,2 por cento. Este ultimo teste mostrou-se aplicavel no diagnostico sorologico da Doenca de Chagas.


Subject(s)
Infant, Newborn , Child, Preschool , Child , Adult , Infant , Adolescent , Middle Aged , Humans , Animals , Chagas Disease/epidemiology , Brazil/epidemiology , Chagas Disease/diagnosis , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique , Prevalence
4.
Rev Inst Med Trop Sao Paulo ; 33(3): 193-8, 1991.
Article in English | MEDLINE | ID: mdl-1844534

ABSTRACT

A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.


Subject(s)
Chagas Disease/epidemiology , Adolescent , Adult , Aged , Animals , Brazil/epidemiology , Chagas Disease/diagnosis , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Female , Fluorescent Antibody Technique , Humans , Infant , Infant, Newborn , Male , Middle Aged , Prevalence
5.
Rev Soc Bras Med Trop ; 22(2): 73-9, 1989.
Article in Portuguese | MEDLINE | ID: mdl-2517851

ABSTRACT

Triatoma brasiliensis, Triatoma pseudomaculata and Rhodnius nasutus were captured in artificial and natural echotopes in ten localities situated in rural areas of Castelo do Piauí and Pedro II, State of Piauí, Brazil. Nymphal instars predominated and birds were the main blood meal source for triatomines. T. brasiliensis was the only triatomine captured inside the houses searched, harbouring flagellates morphologically and biologically indistinguishable from Trypanosoma (Schizotrypanum) cruzi. Specific serology against T. cruzi by indirect immunofluorescence (IFI) test showed 21.7% positive reactions among 566 inhabitants examined. The results suggest that in these localities the active transmission of Chagas' disease occurs.


Subject(s)
Insect Vectors/parasitology , Triatoma/parasitology , Triatominae/parasitology , Trypanosoma cruzi/isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , Animals , Brazil/epidemiology , Chagas Disease/epidemiology , Child , Child, Preschool , Feeding Behavior , Female , Humans , Infant , Infant, Newborn , Insect Vectors/physiology , Male , Middle Aged , Rural Health , Triatoma/physiology
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