Subject(s)
Chlamydia Infections/drug therapy , Chlamydia trachomatis , Endometritis/microbiology , Josamycin/therapeutic use , Abortion, Spontaneous , Adult , Anti-Bacterial Agents , Clinical Trials as Topic , Endometritis/drug therapy , Female , Humans , Infertility, Female , Pregnancy , Pregnancy, Ectopic , Treatment OutcomeABSTRACT
Fifty-two women with unexplained infertility and 55 controls with recently proved fertility were screened for Chlamydia trachomatis (CT) infection in the blood and genital tract. Serum antibody titration was performed with indirect fluorescence. Cell cultures were performed to screen for CT in urethral and endocervical swabs, in endometrial samples taken without endocervical contamination and in salpingeal and/or peritoneal fluid samples. Anti-CT serum antibodies were detected in 36.5% of the patients; CT was isolated in urethral cultures in 26.9%, endocervical cultures in 23.1%, endometrial cultures in 25% and endosalpingeal and/or peritoneal fluid cultures in 1.9%. Comparison of the results in the patients and controls showed a significant difference in the incidence of CT infection in endometrial, urethral and endocervical cultures. Chlamydial endometritis could have been the direct cause of infertility in the patients studied or merely might have indicated endosalpingitis that was not detectable at laparoscopy.
Subject(s)
Chlamydia Infections/diagnosis , Endometritis/diagnosis , Infertility, Female/etiology , Adult , Antibodies, Bacterial/analysis , Chlamydia Infections/blood , Chlamydia Infections/complications , Chlamydia trachomatis/immunology , Endometritis/blood , Endometritis/complications , Female , HumansABSTRACT
Leptospirosis, anthropo-zoonosis ubiquitously widespread, is a social and economic problem still to be solved. The experimental and therapeutic employment of many antibiotics has largely been tested "in vitro" and "in vivo". In the following research we tried to evaluate, by experimental "in vitro" method, the sensitivity difference of three serovar strains of Leptospira interrogans to two macrolides, Erythromycin and Josamycin, compared with Penicillin. From standard cultures, previously treated with serial dilution of these antibiotics, the MIC and MSC, as quantitative parameters, have been stated. For the qualitative evaluation of the damages induced at ultrastructural level by the drug activity. Electron Microscopy investigations were performed on specimens from cultures treated for 6 hr with twice and tenfold the MSC. The present research confirms the good sterilizing efficaciousness "in vitro" of the tested macrolides (MSC less than 1 mcg/ml) and their different activity pathway.
Subject(s)
Erythromycin/pharmacology , Josamycin/pharmacology , Leptospira interrogans/drug effects , Penicillins/pharmacology , Drug Resistance, Microbial , Leptospira interrogans/classification , Leptospira interrogans/ultrastructure , Penicillin Resistance , Species SpecificityABSTRACT
A preliminary research, in order to evaluate by immunofluorescent technique the prevalence of heart specific and non specific autoantibodies, has been performed on a population of 58 cardiac patients, allograft applying. Two other populations (cardiac patients not allograft candidates, and healthy blood donors), were also tested as controls. Significant differences in the presence of specific anti-heart antibodies were revealed among the three populations (89.7, 39.1 and 10%, respectively). No relevant correlation has appeared with the antibody titer between the two groups of cardiac patients.
Subject(s)
Autoantibodies/analysis , Heart Transplantation , Myocardium/immunology , Autoantibodies/immunology , Heart Diseases/immunology , Humans , Preoperative CareSubject(s)
Alkaloids/pharmacology , Cell Survival/drug effects , Animals , Cells, Cultured , Chick Embryo , Haplorhini , Plants, MedicinalABSTRACT
The aggregation and condensation of ribosomes and the disjunction of the cell-wall membranous system are the ultrastructural alterations caused by rifampicin on B. melitensis cultured in Brucella-Broth medium. Our ultrastructural researches carried out on mouse peritoneal macrophages infected with B. melitensis and treated with rifampicin (1 microgram/ml) have demonstrated that vacuoles containing B. melitensis which had been damaged by the drug fuse with lysosomes. On the contrary vacuoles containing undamaged and viable Brucellae showed markedly impaired lysosomal fusion.
Subject(s)
Brucella/drug effects , Macrophages/physiology , Rifampin/pharmacology , Animals , Brucella/ultrastructure , In Vitro Techniques , Lysosomes/ultrastructure , Macrophages/ultrastructure , Mice , Microscopy, Electron , Ribosomes/drug effects , Ribosomes/ultrastructureABSTRACT
The AA. analyze the ultrastructural and immunological patterns of a low virulent strain of T. gondii cultured in vitro on A-9 cells line (PV-H1C-83). The tissue culture method described here is highly recommended to study the immunological aspects of experimental toxoplasmosis. Biological tests' results and the time courses of serological tests are also similar to those obtained from mice infected with cerebral tissue containing cysts. The low virulent strain (PV-H1C-83), when cultured in vitro, is able to induce the formation of large parasitophorous vacuoles, in which the EM investigation shows a typical cyst wall enveloping numerous bradyzoites.
Subject(s)
Toxoplasma/immunology , Animals , Antibody Formation , Cell Line , Female , Humans , Mice , Microscopy, Electron , Toxoplasma/ultrastructure , Toxoplasmosis/immunologyABSTRACT
A human Toxoplasma strain, isolated in mice from bioptic lymphoglandular tissue, has been cultured in vitro in continuous cell lines. Its presence in some parastized cells, with parasitophorous vacuoles surrounded by a cyst-like wall and filled with a cystozoite-like clone, was observed by electron microscopy. These characteristic and constant features were confined in different cell line cultures and are suggested as possible markers of low virulent strains.
Subject(s)
Organoids/parasitology , Toxoplasma/pathogenicity , Vacuoles/parasitology , Animals , Brain/parasitology , Cell Line , Chlorocebus aethiops , Endoplasmic Reticulum/ultrastructure , Lysosomes/ultrastructure , Membranes/ultrastructure , Toxoplasma/growth & development , Toxoplasma/ultrastructureABSTRACT
We have devised a rapid and inexpensive gel filtration method to separate IgM fractions from small amounts of serum. These fractions have been titrated with IgM-IFAT and IgM-IHAT, and their titers have been compared with those obtained on whole serum. The results show that the separation of IgM from IgG allows detection of IgM antibodies in many cases of acquired toxoplasmosis, especially in cases of congenital toxoplasmosis. False positive results due to the presence of rheumatoid factors are also avoided. The IHA test performed on IgM fractions is constantly negative.
Subject(s)
Immunoglobulin M/isolation & purification , Toxoplasmosis, Congenital/diagnosis , Toxoplasmosis/diagnosis , Adult , Chromatography, Gel , Fluorescent Antibody Technique , Hemagglutination Tests , Humans , Immunoglobulin M/analysis , Infant, Newborn , Toxoplasma/immunologyABSTRACT
Through the use of the Limulus test research has been carried out on gram-negative endotoxin in patients with hepatic cirrhosis, chronic hepatitis, acute hepatitis, and in a control group. The positivity of this test in patients with cirrhosis and chronic hepatitis was 93.3% and in cases of acute hepatitis it was 90.9%. The effect of the combined administration of lactulose and paromomycin on endotoxin blood levels has been evaluated in a group of 9 patients with acute hepatitis, 8 with cirrhosis, 1 in hepatic coma, and 1 patient with chronic persistent hepatitis: in 18 of the 19 patients the Limulus test became negative. The results have been discussed in relation to clinical and laboratory data, and to recent data concerning the interaction between intestinal bacterial flora, endotoxin, and liver. Hypotheses have been proposed regarding the hepatocellular c-AMP mediated mechanism of endotoxin action.
Subject(s)
Disaccharides/therapeutic use , Endotoxins/blood , Lactulose/therapeutic use , Liver Diseases/blood , Paromomycin/therapeutic use , Chronic Disease , Hepatitis/blood , Hepatitis/diagnosis , Hepatitis/drug therapy , Hepatitis B/blood , Hepatitis B/diagnosis , Hepatitis B/drug therapy , Humans , Limulus Test , Liver Cirrhosis/blood , Liver Cirrhosis/diagnosis , Liver Cirrhosis/drug therapy , Liver Diseases/diagnosis , Liver Diseases/drug therapySubject(s)
Protozoan Infections/drug therapy , Amebiasis/drug therapy , Balantidiasis/drug therapy , Entamoeba histolytica , Entamoebiasis/drug therapy , Giardiasis/drug therapy , Humans , Leishmaniasis/drug therapy , Malaria/drug therapy , Toxoplasmosis/drug therapy , Trichomonas Infections/drug therapyABSTRACT
Chemotaxis of human leukocytes was inhibited in vitro by four non-steroidal anti-inflammatory drugs, namely, lysine acetylsalicylate, phenylbutazone, indomethacin, and indoprofen. Dose-dependent effects were always found and significant regression was proved for all drugs except phenylbutazone.