1.
Minerva Med
; 71(6): 401-4, 1980 Feb 25.
Article
in Italian
| MEDLINE
| ID: mdl-6928510
ABSTRACT
A new enzymatic fluorimetric assay for the determination of total serum bile acids is described. The enzyme 3 alpha-idroxysteroid NAD oxireductase by oxydising the free OH in position 3 alpha of bile acid molecule, reduce NAD previously added. Afterwards the hydrogen of the generated NADH is transferred by a diaphorase to resazurin to yield the fluorophore resorufin proportionally to the amount of bile acids in the sample. The assay is simple, accurate, without bilirubin's influence and useful for a routine use because the analysis time is not different from the more common laboratory tests.
Subject(s)
3-Hydroxysteroid Dehydrogenases , Bile Acids and Salts/blood , Spectrometry, Fluorescence , 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) , Adult , Bile Acids and Salts/analysis , Female , Humans , Male
2.
Quad Sclavo Diagn
; 9(1): 307-20, 1973 Mar.
Article
in Italian
| MEDLINE
| ID: mdl-4788718
3.
Experientia
; 27(1): 33-4, 1971 Jan 15.
Article
in English
| MEDLINE
| ID: mdl-5549230
Subject(s)
DNA/biosynthesis , Lectins/pharmacology , Lymphocytes/metabolism , Methotrexate/pharmacology , Thymidine Kinase/metabolism , Autoradiography , Humans , In Vitro Techniques , Lymphocytes/drug effects , Lymphocytes/enzymology , Phosphorus Isotopes , RNA/biosynthesis , Stimulation, Chemical , Thymidine Kinase/blood , Tritium
4.
Minerva Med
; 61(37): 2005-12, 1970 May 19.
Article
in Italian
| MEDLINE
| ID: mdl-5469250
Subject(s)
Chromosome Aberrations , Leukemia, Plasma Cell/genetics , Leukemia, Plasma Cell/physiopathology , Aged , Autoradiography , Bence Jones Protein/analysis , Blood Cell Count , Blood Coagulation Tests , Blood Protein Electrophoresis , Culture Techniques , Diagnosis, Differential , Humans , Karyotyping , Leukemia, Plasma Cell/diagnosis , Leukemia, Plasma Cell/immunology , Leukemia, Plasma Cell/therapy , Male , Middle Aged , Multiple Myeloma/diagnosis , Thymidine
5.
Boll Soc Ital Biol Sper
; 46(4): 163-6, 1970 Feb 28.
Article
in Italian
| MEDLINE
| ID: mdl-5516260
6.
Boll Soc Ital Biol Sper
; 46(4): 167-70, 1970 Feb 28.
Article
in Italian
| MEDLINE
| ID: mdl-5516261
7.
Boll Soc Ital Biol Sper
; 46(4): 157-9, 1970 Feb 28.
Article
in Italian
| MEDLINE
| ID: mdl-5280556