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1.
Micron ; 56: 73-9, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24231674

ABSTRACT

This study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocytochemical techniques. The investigation was followed by characterization of the chemical properties of the liquid crystals (LC) after immersion in cell culture media using Fourier transform infrared spectroscopy (FTIR). The surface morphology of cells adhered to the LC was studied using atomic force microscopy (AFM). Consistent with the expressions of the integrins α2, α3 and ß1, extracellular matrix proteins (laminin, collagen type IV and fibronectin) were found secreted by the HaCaT onto CELC and these proteins were also secreted by cells cultured on the glass substrates. FTIR analysis of the LC revealed the existence of spectrum assigned to cholesterol and ester moieties that are essential compounds for the metabolizing activities of keratinocytes. The immunostainings indicated that cell adhesion on the LC is mediated by self-secreted ECM proteins. As revealed by the AFM imaging, the constraint in cell membrane spread on the LC leads to the increase in cell surface roughness and thickness of cell membrane. The biophysical expressions of cells on biocompatible CELC suggested that CELC could be a new class of biological relevant material.


Subject(s)
Cell Culture Techniques , Cholesterol Esters/metabolism , Keratinocytes/metabolism , Liquid Crystals , Cell Adhesion , Cell Line , Cell Membrane/metabolism , Collagen Type IV/biosynthesis , Extracellular Matrix Proteins/biosynthesis , Fibronectins/biosynthesis , Humans , Integrin alpha2/biosynthesis , Integrin alpha3/biosynthesis , Integrin beta1/biosynthesis , Laminin/biosynthesis , Microscopy, Atomic Force , Spectroscopy, Fourier Transform Infrared
2.
Exp Cell Res ; 319(4): 390-401, 2013 Feb 15.
Article in English | MEDLINE | ID: mdl-23262023

ABSTRACT

Reducing Cx43 expression stimulates skin wound healing. This is mimicked in models when Cx43 function is blocked by the connexin mimetic peptide Gap27. IGF-I also stimulates wound healing with IGFBP-5 attenuating its actions. Further, the IGF-I to IGFBP-5 ratio is altered in diabetic skin, where wound closure is impaired. We investigated whether Gap27 remains effective in augmenting scrape-wound closure in human skin wound models simulating diabetes-induced changes, using culture conditions with raised glucose, insulin and IGFBP-5. Gap27 increased scrape-wound closure in normal glucose and insulin (NGI) and to a lesser extent in high glucose and insulin (HGI). IGF-I enhanced scrape-wound closure in keratinocytes whereas IGFBP-5 inhibited this response. Gap27 overcame the inhibitory effects of IGFBP-5 on IGF-I activity. Connexin-mediated communication (CMC) was reduced in HGI, despite raised Cx43, and Gap27 significantly decreased CMC in NGI and HGI. IGF-I and IGFBP-5 did not affect CMC. IGF-I increased keratinocyte proliferation in NGI, and Gap27 increased proliferation in NGI to a greater extent than in HGI. We conclude that IGF-I and Gap27 stimulate scrape-wound closure by independent mechanisms with Gap27 inhibiting Cx43 function. Gap27 can enhance wound closure in diabetic conditions, irrespective of the IGF-I:IGFBP-5 balance.


Subject(s)
Cell Movement/drug effects , Connexins/pharmacology , Glucose/pharmacology , Insulin-Like Growth Factor Binding Protein 5/pharmacology , Insulin/pharmacology , Skin Physiological Phenomena/drug effects , Cell Culture Techniques , Cell Migration Assays , Cell Movement/physiology , Cells, Cultured , Dose-Response Relationship, Drug , Humans , Infant, Newborn , Keratinocytes/cytology , Keratinocytes/drug effects , Models, Theoretical , Oligopeptides , Osmolar Concentration , Up-Regulation/drug effects , Wound Healing/drug effects
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