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1.
Anal Bioanal Chem ; 402(2): 593-600, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21904801

ABSTRACT

Carbon nanoparticles (CNPs) labeled with reporter molecules can serve as signaling labels in rapid diagnostic assays as an alternative to gold, colored latex, silica, quantum dots, or up-converting phosphor nanoparticles. Detailed here is the preparation of biomolecule-labeled CNPs and examples of their use as a versatile label. CNPs can be loaded with a range of biomolecules, such as DNA, antibodies, and proteins (e.g., neutravidin or a fusion protein of neutravidin with an enzyme), and the resulting conjugates can be used to detect analytes of high or low molecular mass.


Subject(s)
Carbon/chemistry , Diagnostic Tests, Routine/methods , Immunoassay/methods , Nanoparticles/chemistry , Staining and Labeling/methods , Animals , Humans
2.
J Agric Food Chem ; 52(21): 6633-9, 2004 Oct 20.
Article in English | MEDLINE | ID: mdl-15479033

ABSTRACT

Heterologous conjugates of wheat arabinoxylan and beta-casein were prepared via enzymatic cross-linking, using sequential addition of the arabinoxylan to a mixture of beta-casein, peroxidase, and hydrogen peroxide. The maximal formation of adducts between the beta-casein and the feruloylated arabinoxylan was reached at a protein-to-arabinoxylan ratio of 10:1, in combination with a molar ratio hydrogen peroxide to substrate of 2:1 and a molar protein-to-enzyme ratio between 10(2) and 10(4). The protein-arabinoxylan adducts were separated from the arabinoxylan homopolymers by size exclusion and anion exchange chromatography. The molar ratio protein:arabinoxylan in the purified conjugates varied between 0.1 and 5.6. This is the first report on the large-scale enzymatic preparation of heterologous protein-arabinoxylan conjugates.


Subject(s)
Caseins/metabolism , Coumaric Acids/metabolism , Horseradish Peroxidase/metabolism , Xylans/metabolism , Cross-Linking Reagents , Spectroscopy, Fourier Transform Infrared , Triticum/chemistry
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