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1.
N Biotechnol ; 82: 1-13, 2024 Sep 25.
Article in English | MEDLINE | ID: mdl-38615946

ABSTRACT

This work proposes a new data-driven model to estimate and predict pH dynamics in freshwater raceway photobioreactors. The resulting model is based purely on data measured from the reactor and divides the pH dynamics into two different behaviors. One behavior is described by the variation of pH due to the photosynthesis phenomena made by microalgae; and the other comes from the effect of CO2 injections into the medium for control purposes. Moreover, it was observed that the model parameters vary throughout the day depending on the weather conditions and reactor status. Thus, a decision tree algorithm is also developed to capture the parameter variation based on measured variables of the system, such as solar radiation, medium temperature, and medium level. The proposed model has been validated for a data set of more than 100 days during 10 months in a semi-industrial raceway reactor, covering a wide range of weather and system scenarios. Additionally, the proposed model was used to design an adaptive control algorithm which was also experimentally tested and compared with a classical fixed parameter control approach.


Subject(s)
Microalgae , Microalgae/metabolism , Microalgae/growth & development , Hydrogen-Ion Concentration , Photobioreactors , Algorithms , Bioreactors , Models, Biological , Photosynthesis
2.
N Biotechnol ; 77: 58-67, 2023 Nov 25.
Article in English | MEDLINE | ID: mdl-37467926

ABSTRACT

In this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different genera of microalgae, having been trained with several species of each genus. It was further complemented with a classification threshold to discard unwanted objects while improving the overall accuracy of the model. The model achieved an accuracy of up to 97.27% when classifying a culture. The results demonstrate the effectiveness of the Deep Learning models for the characterization of microalgae cultures, it being a useful tool for the monitoring of microalgae cultures in large-scale production facilities while providing accurate characterization over a wide range of genera.


Subject(s)
Artificial Intelligence , Microalgae , Neural Networks, Computer , Biomass
3.
Biotechnol Bioeng ; 118(3): 1186-1198, 2021 03.
Article in English | MEDLINE | ID: mdl-33270219

ABSTRACT

Temperature and irradiance are the two most relevant factors determining the performance of microalgae cultures in open raceway reactors. Moreover, inadequate temperature strongly reduces the biomass productivity in these systems even if enough sunlight is available. Controlling the temperature in large open raceway reactors is considered unaffordable because of the large amount of energy required. This study presents an indirect method for temperature regulation in microalgae raceway reactors by optimizing the culture depth. First, the effect of the culture depth on the raceway temperature is analyzed for different seasons of the year. Afterward, a simulation study is presented where the proposed control approach is compared to the normal operation mode with constant volume in the reactor. This study is also extended to industrial scale. Relevant improvements on the temperature factor and biomass production are presented. The developed knowledge allows the improvement of the performance in open raceway reactors up to 12% without involving additional energy and costs, being a suitable solution for large industrial reactors that until now have no options for controlling the temperature.


Subject(s)
Bioreactors , Cell Culture Techniques , Computer Simulation , Hot Temperature , Models, Biological , Scenedesmus/growth & development , Biomass
4.
Water Sci Technol ; 82(6): 1155-1165, 2020 Sep.
Article in English | MEDLINE | ID: mdl-33055405

ABSTRACT

The pH control in raceway reactors is crucial for an optimal performance of the system. Classical pH control is exclusively performed during the daytime period for cost saving reasons. This paper demonstrates that pH can be controlled 24 hours a day by using both a continuous-based and an event-based control approach, being able to improve the system's performance and reducing costs at the same time. Thus, experimental tests on a raceway reactor for several days are presented to show a comparison between traditional control algorithms during the daytime period versus an event-based control approach operating during both daytime and night-time periods. As a result, the combination of classical PI control for the daytime period and the event-based control for the night-time period is presented as a promising pH control architecture in raceway reactors.


Subject(s)
Microalgae , Algorithms , Hydrogen-Ion Concentration
5.
ISA Trans ; 99: 454-464, 2020 Apr.
Article in English | MEDLINE | ID: mdl-31662184

ABSTRACT

Temperature control in buildings is usually driven by energy conservation although the occupants' comfort is also important considering its impact on productivity and health. However, energy efficiency and comfort are opposing objectives and therefore this type of problem can be resolved by means of a multiobjective optimization approach. The simulations we carried out indicate that set points optimization has the potential to reduce energy consumption in the order of 10% while also providing a comfortable work environment for the occupants.

6.
ISA Trans ; 65: 525-536, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27593955

ABSTRACT

In this work, an application of the Symmetric Send-On-Delta (SSOD) event-based controllers to the inside air temperature control of the greenhouse production process is presented. The control technique analysis is split into two stages. The first stage is devoted to determine the proper controller parameters and to check the influence of the Send-On-Delta (SOD) threshold value through simulation study. At the second stage, experimental tests on the real greenhouse facilities are performed. The obtained results show that the analyzed control techniques handle the control task with desired accuracy and performance. In particular, the proposed control system saves costs related with energy consumption and wear minimization, by achieving a satisfactory performance at the same time.

7.
Bioresour Technol ; 170: 1-9, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25113401

ABSTRACT

This work addresses effective utilization of flue gases through the proper pH control in raceway reactors. The pH control problem has been addressed with an event-based control approach using a Generalized Predictive Controller (GPC) with actuator deadband. Applying this control strategy it is possible to reduce the control effort, and at the same time saving control resources. In the pH process case, the event-based controller with actuator deadband can be tuned to supply only necessary amount of CO2 to keep the pH close to its optimal value. On the other hand, the evaluated control algorithm significantly improves the pH control accuracy, what has a direct influence on biomass production. In order to test the performance of the event-based GPC controller, several experiments have been performed on a real raceway reactor. Additionally, several control performance indexes have been used to compare the analyzed technique with commonly used on/off controller.


Subject(s)
Algorithms , Bioreactors/microbiology , Biotechnology/methods , Cell Culture Techniques/methods , Gases/chemistry , Microalgae/growth & development , Biomass , Hydrogen-Ion Concentration , Photochemical Processes
8.
Bioresour Technol ; 126: 172-81, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23073105

ABSTRACT

A dynamic model for microalgal culture is presented. The model takes into account the fluid-dynamic and mass transfer, in addition to biological phenomena, it being based on fundamental principles. The model has been calibrated and validated using data from a pilot-scale tubular photobioreactor but it can be extended to other designs. It can be used to determine, from experimental measurements, the values of characteristic parameters. The model also allows a simulation of the system's dynamic behaviour in response to solar radiation, making it a useful tool for design and operation optimization of photobioreactors. Moreover, the model permits the identification of local pH gradients, dissolved oxygen and dissolved carbon dioxide; that can damage microalgae growth. In addition, the developed model can map the different characteristic time scales of phenomena inside microalgae cultures within tubular photobioreactors, meaning it is a valuable tool in the development of advanced control strategies for microalgae cultures.


Subject(s)
Microalgae/growth & development , Models, Biological , Photobioreactors/microbiology , Scenedesmus/growth & development , Biomass , Calibration , Carbon Dioxide/analysis , Computer Simulation , Hydrogen-Ion Concentration , Oxygen/analysis , Time Factors
9.
Biotechnol Bioeng ; 84(5): 533-43, 2003 Dec 05.
Article in English | MEDLINE | ID: mdl-14574687

ABSTRACT

The optimization of carbon use in pilot-scale outdoor tubular photobioreactors is investigated in this study. The behavior of a 0.20-m(3) tubular photobioreactor was studied, with and without algae, by steady-state and pulse dynamic-response analysis experiments. A model of the system was obtained and implemented in a programmable control unit and was used to control the reactor under normal production conditions. Results showed that, using and on-off control, the mean daily CO(2) flow in the reactor was 0.86 g min(-1), 19.7% of this being lost. By using a predictive control algorithm the mean daily CO(2) flow was reduced to 0.74 g min(-1), with losses being reduced to 15.6%. In this case, pH tracking was not adequate, especially at the beginning and end of the daylight period, because the variation in solar irradiance was not considered. Taking solar irradiance into account resulted in better performance, with mean daily CO(2) flow reduced to 0.70 g min(-1), and carbon losses reduced to 5.5%. pH tracking was improved and valve actuation was reduced. Improvement of pH control reduced pH gradients in the culture, which increased the photosynthesis rate and biomass productivity of the system. Biomass productivity increased from 1.28 to 1.48 g L(-1) day-(1) when on-off control was replaced by model-based predictive control plus solar irradiance effect mode. Implementation of this methodology in outdoor photobioreactors can increase productivity by 15% and reduce the cost of producing biomass by >6%. Clearly, application of effective control techniques, such as model-based predictive control (MPC), must be considered when developing these processes.


Subject(s)
Algorithms , Bioreactors/microbiology , Carbon/metabolism , Cell Culture Techniques/methods , Diatoms/growth & development , Diatoms/metabolism , Models, Biological , Cell Culture Techniques/instrumentation , Computer Simulation , Diatoms/radiation effects , Equipment Failure Analysis , Feasibility Studies , Feedback/physiology , Oxygen Consumption/physiology , Photobiology/instrumentation , Photobiology/methods , Pilot Projects , Sunlight
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