[Cellular and humoral immunity in rats with atopic dermatitis].

We studied indicators of systemic and local immunity in rats with allergic dermatitis (Ig A, E, G, M, CIC, CD3, CD4, CD8, SD 19, SD56). In rats with allergic dermatitis on the local level, the most active is humoral immunity. One of the pathogenesis of atopic dermatitis is an increased content of circulating immune complexes in the blood and decreased T-helper in a total amount of T- lymphocytes. An informative index with a high level of correlation is the IgE level, confirming the advisability of its determination in blood plasma in acute dermatitis to characterize lymphocyte apoptosis markers and prognosis of the disease. An established negative correlation between the concentrations of SD95-marker of apoptosis and the IgE level in healthy and sick animals may point for involvement of apoptosis in the regulation of the total IgE.

[Deformability of the erythrocytes membrane in rats of different age in hypoxia].

Using modern ("laser tweezers", low ionic strength Liss, definition of microviscosity of erythrocyte membranes by fluorescence probe pyrene) and classical methods of investigation the osmotic resistance of red blood cells and protein membrane of red blood cells, the authors have esteblisheshed that deformation of the red blood cells in hemic hypoxia varies depending on the animals' age. The maximum capacity of red blood cells to deform was detected in rats of prepubertal period (40.2%). In the group of old rats this parameter reached the minimum level (19.7%). Age changes in erythrocyte membranes during hypoxia are manifested in an increase in the viscosity of the lipid bilayer (microviscosity index reduced in old rats compared with young adult animals by 69.5%) and an increase in the percentage of skeletal protein spectrin (by 53.0%). These changes determine the reduction in deformation capacity of red blood cells. The relationship between the age-related changes of erythrocyte membrane deformation and the state of regional microcirculation has been determined. In old rats a perfusion volume is reduced by 43.8%, the fractional volume of red blood cells passing per unit time through microvessels reduced by 59.3%, the proportion of oxygen consumption in tissues decreased by 6.6% and the perfusion of oxygen saturation decreased by 50.0%.

[The mechanism of the antihypoxic action of indomethacin, voltaren and ibuprofen]. / K mekhanizmu antigipoksicheskogo deistviia indometatsina, vol'tarena i ibuprofena.

The antihypoxic effects of indomethacin, voltaren and ibuprofen were studied experimentally by the parameters of acid-base equilibrium of the blood and the ratio of lactic and pyruvic acids in the blood during chronic hemic and respiratory hypoxia. The results of the studies suggested that indomethacin, voltaren and to a lesser extent ibuprofen in the tested doses (10% LD50) exerted the antihypoxic action that consisted in the improvement of oxygen supply to tissues under conditions of its deficiency, the decrease of incompletely oxidized products of metabolism, the enhancement of hemoglobin synthesis and the ability of voltaren to influence the increase of the number of erythrocytes in the peripheral blood.

[Change in the anti-inflammatory activity of nonsteroidal antiphlogistics in conditions of acute hypoxic hypoxia]. / Izmenenie protivovospalitel'noi aktivnosti nesteroidnykh antiflogistikov v usloviiakh ostroi gipoksicheskoi gipoksii.

It has been shown on animals with experimental histamine edema under acute hypoxic hypoxia that anti-inflammatory properties of the known non-steroidal antiphlogistics and new derivatives of fumaric acid heterylamides administered in a dose of 10% of the LD50 underwent changes. At "altitudes" of 5000 and 10000 m a high enough anti-inflammatory activity was preserved by the drugs that, apart from an anti-exudative action, produced an antihypoxic one. The other non-steroidal anti-inflammatory drugs under study demonstrated either a lowered activity or completely lost it, depending on the "altitude".

[Effect of nonsteroid anti-inflammatory agents on the permeability of histohematic barriers]. / Vliianie nesteroidnykh protivovospalitel'nykh sredstv na pronitsaemost' gistogematicheskikh bar'erov.

It has been demonstrated on male rats that non-steroidal anti-inflammatory drugs change the permeability for 32P of histohematic barriers (HHB) of the internal organs of normal rats and animals with hyperergic inflammation. The nature of the effect appears to be due to the morphological structure of the HHB and blood flow velocity at the organ level. In hyperergic inflammation, HHB permeability for 32P rises 1.5--2-fold. The action of non-steroidal antiphlogistics is organ specific in hyperergic inflammation.