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1.
J Pharm Biomed Anal ; 70: 401-7, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22776736

ABSTRACT

To support a first-in-human (FIH) clinical study in healthy volunteers, a human plasma assay, a 20-fold more sensitive method than the validated non-clinical LC-MS/MS assays, was requested. For the clinical assay, a LLOQ of 0.050 ng/mL for Compound A and 0.100 ng/mL for Compound B was desired to accurately determine the analyte concentrations in human plasma samples across all treatment groups. A design of experiment (DOE) investigation was performed in an effort to optimize the extraction procedure of the bioanalytical assay used to support the first in human study and future clinical studies. Three factors, extraction buffer pH (two pHs), volume ratio of organic solvent to plasma (two ratios), and extraction shake time (three times), were selected for the DOE. Both analytes were analyzed at a low concentration, 0.150 ng/mL, and a stable isotope label internal standard was used for each analyte. To estimate the recovery of each analyte from the extraction, the response ratio of each analyte over the respective internal standard was used, and to estimate matrix effects, the absolute response (peak area) of each analyte was used. The results of the DOE indicated that the three factors tested had a more significant effect on the extraction of the metabolite, Compound B, compared to that of the parent, Compound A. The extraction buffer pH had the greatest influence on Compound B and the volume of extraction solvent had an influence on both analytes. Unexpectedly, a longer extraction time caused an apparent decrease in the overall recovery for both analytes. This was presumably due to an increased extraction of interfering matrix components. Optimal conditions were achieved for the combined analysis of both compounds using the DOE approach.


Subject(s)
Biological Assay/methods , Biomarkers/blood , Chromatography, High Pressure Liquid , Research Design , Tandem Mass Spectrometry , Biological Assay/standards , Buffers , Calibration , Chromatography, High Pressure Liquid/standards , Humans , Hydrogen-Ion Concentration , Models, Statistical , Reference Standards , Reproducibility of Results , Sensitivity and Specificity , Solvents/chemistry , Tandem Mass Spectrometry/standards , Time Factors
2.
J Ind Microbiol Biotechnol ; 34(3): 193-202, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17131104

ABSTRACT

BMS-353645, also known as sordarin, was of interest based on its activity against pathogenic fungi. The objective of these studies was to provide high quality starting substrate for chemical modification aimed at further improving biological activity, with particular interest in the inhibition of Aspergillus. In the work presented here, Design of Experiments, or DOE, was successfully combined with traditional approaches to significantly improve sordarin yields in fermentation flasks. Overall, yields were increased 25-fold from <100 microg/g to as high as 2,609 microg/g in flasks through the use of various medium and conduction changes supplemented with DOE. The improved process was then successfully scaled to pilot plant tanks with the best batch producing 2,389 microg/g sordarin at the 250-l scale.


Subject(s)
Fermentation , Indenes/metabolism , Sordariales/metabolism , Research Design
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