ALTERATION OF OXIDATIVE METABOLISM AND IMMUNOLOGICAL PARAMETERS UNDER THE INFLUENCE OF UVA RADIATION IN WOMEN OF DIFFERENT AGES.

Purpose of study was to investigate the alteration of oxidative and immunological metabolism in blood of reproductive and menopausal age women after UVA irradiation. Women of two groups (1 - menopausal, 2 - reproductive age) were exposed to radiation with wave length 320-400 nm for 5-10 minutes per session in the solarium for the period of 3 months (6 days per month).. Parameters of oxidative metabolism - activity of red blood cells (RBC), antioxidant enzymes catalase (CAT) and superoxiddismutase (SOD) were studied by spectrophotometric method; reactive oxygen (O2-) and lipid (LOO/) free radicals content in whole blood were studied by Electron Paramagnetic Resonance (EPR) methods with use of specific spin-traps - 5,5-Dimethyl-1-Pyrroline-N-Oxide (DMPO) and α-phenyl-N-tert-butylnitrone (PBN) (SIGMA). Parameters of immunological metabolism - IFN-α, IL-2, IL-10 cytokines content in blood were studied by immunoenzymatic assay ELISA. The study protocol has been approved by the Ethical Committee of the Tbilisi State Medical University. After the course of UVA irradiation in menopausal women CAT activity increased by 20%, SOD - by 24%, and EPR signal of spin trapped lipoperoxide radicals was detected. No alterations in blood redox-balance were detected in women of reproductive age. Values of blood immunological parameters in menopausal women were not changed under the influence of UV radiation; in women of reproductive age IL-10 content increase by 93% (within a normal value range) was revealed. Research results have shown that UVA rays cause a particularly significant influence on the oxidative metabolism in the women of menopausal age. Increase of IL-10 blood levels in women of reproductive age (considering its imunosupressing activity) represents an additional risk of cancerogenesis. Based on the study results, we recommend avoiding UV (including UVA) radiation procedures to women of both reproductive and menopausal ages.

[Role of redox- and hormonal metabolism in the mechanisms of skin aging].

The aim of the study was to investigate the role of redox balance in the pathogenesis of skin aging in menopausal women. 30 menopausal women aged 40 to 55 years and 30 reproductive women aged 25 to 35 years were studied. Qualitative assessment of the skin (moisture, fat, elasticity) was performed; in the venous blood hormonal metabolism indicators: estradiole - E, testosterone - T, follicle stimulating hormone - FSH and redox parameters - oxygen and lipid free radical content (EPR method), antioxidant enzymes (catalase, superoxide dismutase (SOD) and glutationreducrase (GR)) activity (spectroscopic method) were studied. According results of the study, in menopausal women statistically significant loss of skin elasticity and increase the number of pores was revealed in comparison to the reproductive women. These changes occur against the background of statistically significant increase of the blood testosterone and FSH content; estradiol in women menopausal period has tendency to decrease. Redox indicators of blood did not differ statistically significant in women of reproductive and menopausal period, although there was a tendency to increase the activity of catalase and GR in menopausal women period, indicating on the intensification of oxidative processes in this age group. Statistically significant negative correlation between blood estradiole content and SOD's activity (r=-0.413, p=0.0017) and positive correlation between blood estradiole content and GR activity (r=0.565, p=0.002) was revealed. Decrease in the estradiol concentration and disbalance in redox-system in the women's blood correlats with the rate of pigmented spots growth and decrease of the skin moisture. It is concluded that in mechanisms of skin aging of menopausal women estrogen-depending alterations in redox-balance places important role.

Nitric oxide dependent skin aging mechanism in postmenopasal women.

The goal of our study was to determine nitric oxide (NO)-dependent mechanisms of skin aging in post- and premenopausal women. 23 women (age 40-50 year) were investigated. Women were divided into 2 groups: I group - reproductive age women (10 patients); II group - menopause age women (13 patients). Patients were included in the menopausal group when they fulfill the following criteria: at least 12 month of amenorrhea. All patients are subjected to a standard diagnostic protocol. In each group we investigated estradiole, lipids and free NO content. Skin of postmenopausal women as a rule was pale, grayish, in some cases revealed pigmentation; skin around the eyes less flexible, wrinkled. Moderate hypercholesterolemia and reduced levels of nitric oxide and estradiole in the blood of studed postmenopausal women (in comparison reproductive aged women) was revealed. Decrease free NO content in blood may be due to the decrease of NO synthesis, as well as the oxidative degradation of NO in the oxidative stress conditions. N artcle the mechanism of dependence of NO-vasodilatative system activity on rhe level of hypercholesterolemia and estrogen content in blood are discussed. It was concluded that in menopausal women insufficiency of estrogen's content is the main factor, which stipulates insufficiency of of NO-dependent vasodilatative mechanism, and disorder of subcutaneous vasodilatation in aged (postmenopausal) women which contributes to violations of the oxidative processes in the skin and its rapid aging.