ABSTRACT
The study was carried out with the young (4-5 months) and elderly (15-18 months) female and male rats at the experiment start. The mercury salt was administered into animals with drinking water in 1 mcg/l concentration during 3 months before and 1 month after single X-irradiation with 25 cGy dose. The genotoxic effects in leukocytes were evaluated on the base of the content and structure DNA alterations in 24 hrs and in 1 mth after irradiation. The alaninaminotransferase activity in serum was determined to evaluate functional status of liver. A protector intramuscular injection of Ligfol preparation with the 250 mg/kg dose in 24 hrs before irradiation resulted in increase of the blood leukocyte amount, more expressed in 1 mth after irradiation. The standardizing effect of preparation on the leukocyte DNA content is manifested in 1 mth after combined exposure on rats within young male and elderly female. At the same time, Ligfol showed some restoring effect on the alterations of leukocyte supercoiled DNA structure in 1 mth after irradiation within elderly female rats. The preparation showed the standardizing effect on the liver cells in young males injured by radiation-mercury exposures with low doses, however Ligfol had not influence practically on the destruction degree of hepatocytes after similar injury within female young and elderly rats. So, results of the intramuscular Ligfol injection is manifested at least during 1-2 mths; the young rats of both gender and elderly females showed the most sensitivity to the radiation-mercury exposure with low doses and to the Ligfol, according to the studied indexes.
Subject(s)
Aging , Antioxidants/therapeutic use , Gamma Rays/adverse effects , Lignin/analogs & derivatives , Mercury Compounds/toxicity , Nitrates/toxicity , Radiation Injuries, Experimental , Aging/blood , Aging/drug effects , Aging/radiation effects , Animals , Antioxidants/pharmacology , DNA Damage , Female , Leukocyte Count , Leukocytes/drug effects , Leukocytes/metabolism , Leukocytes/radiation effects , Lignin/pharmacology , Lignin/therapeutic use , Liver/drug effects , Liver/enzymology , Liver/radiation effects , Liver Function Tests , Male , Radiation Injuries, Experimental/blood , Radiation Injuries, Experimental/etiology , Radiation Injuries, Experimental/prevention & control , RatsABSTRACT
The therapeutic effect of olipiphate was demonstrated for chronic inflammation of advanced arthritis and concanavalin A-related acute edema. The best systemic effect was obtained with 50 mg/kg, symptomatic--100 mg/kg. Skin wounds treated with 5% olipiphate (26 + 2) healed faster than those treated with 2% solcoseryl (30 + 0.8) or in control (33 + 0.6). It was shown histologically that the proliferative and antiphlogistic effect of olipiphate involved no scars.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Lignin/analogs & derivatives , Wound Healing/drug effects , Animals , Anti-Inflammatory Agents/administration & dosage , Arthritis, Infectious/drug therapy , Arthritis, Infectious/microbiology , Concanavalin A/adverse effects , Dose-Response Relationship, Drug , Edema/chemically induced , Edema/drug therapy , Freund's Adjuvant , Lignin/administration & dosage , Lignin/pharmacology , Male , Mice , Mice, Inbred CBA , Mycobacterium tuberculosis , Rats , Skin/drug effects , Skin/pathologyABSTRACT
Preventive injections of olipifat to rats (intramuscularly in a single dose of 250 mg/kg 24 h before gamma-exposure in a dose of 7.6 Gy) decrease animal mortality from 70 to 50%, the mean life span of dead rats remained unchanged. Injection of olipifat before exposure in a dose of 5.2 Gy did not change animal mortality in comparison with irradiated controls, but stimulated postradiation recovery of leukocyte count (mainly the granulocytic component and less so the increase in lymphocyte count). No appreciable effects of olipifat on the postradiation changes in individual fractions of blood leukocytes were observed in animals exposed in a dose of 7.6 Gy.
Subject(s)
Gamma Rays , Lignin/analogs & derivatives , Lymphocytes/drug effects , Radiation-Protective Agents/administration & dosage , Animals , Lignin/administration & dosage , Lymphocyte Count , Lymphocytes/radiation effects , Male , Rats , Rats, Inbred StrainsABSTRACT
Olipiphat, which is experimentally capable of antitumor action, did not induce any immediate hypersensitivity, but lowered such response to horse serum; nor did it stimulate delayed hypersensitivity to full Freund adjuvant or sharp venous return after concavalin A. No skin or eye mucosa irritation was reported.
