ABSTRACT
Dilated cardiomyopathy (DCM) encompasses various acquired or genetic diseases sharing a common phenotype. The understanding of pathogenetic mechanisms and the determination of the functional effects of each etiology may allow for tailoring different therapeutic strategies. MicroRNAs (miRNAs) have emerged as key regulators in cardiovascular diseases, including DCM. However, their specific roles in different DCM etiologies remain elusive. Here, we applied mRNA-seq and miRNA-seq to identify the gene and miRNA signature from myocardial biopsies from four patients with DCM caused by volume overload (VCM) and four with ischemic DCM (ICM). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used for differentially expressed genes (DEGs). The miRNA-mRNA interactions were identified by Pearson correlation analysis and miRNA target-prediction programs. mRNA-seq and miRNA-seq were validated by qRT-PCR and miRNA-mRNA interactions were validated by luciferase assays. We found 112 mRNAs and five miRNAs dysregulated in VCM vs. ICM. DEGs were positively enriched for pathways related to the extracellular matrix (ECM), mitochondrial respiration, cardiac muscle contraction, and fatty acid metabolism in VCM vs. ICM and negatively enriched for immune-response-related pathways, JAK-STAT, and NF-kappa B signaling. We identified four pairs of negatively correlated miRNA-mRNA: miR-218-5p-DDX6, miR-218-5p-TTC39C, miR-218-5p-SEMA4A, and miR-494-3p-SGMS2. Our study revealed novel miRNA-mRNA interaction networks and signaling pathways for VCM and ICM, providing novel insights into the development of these DCM etiologies.
Subject(s)
Cardiomyopathy, Dilated , MicroRNAs , RNA, Messenger , Humans , Cardiomyopathy, Dilated/genetics , Cardiomyopathy, Dilated/metabolism , Cardiomyopathy, Dilated/pathology , MicroRNAs/genetics , MicroRNAs/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Gene Regulatory Networks , Male , Gene Expression Profiling , Gene Expression Regulation , Middle Aged , FemaleSubject(s)
Foreign-Body Migration/diagnostic imaging , Foreign-Body Migration/surgery , Heart Septal Defects, Atrial/diagnostic imaging , Heart Septal Defects, Atrial/etiology , Septal Occluder Device/adverse effects , Cardiac Catheterization/adverse effects , Echocardiography , Female , Heart Septal Defects, Atrial/surgery , Humans , Middle Aged , Prosthesis Design , Prosthesis FailureSubject(s)
Heart Valve Prosthesis/adverse effects , Thrombectomy , Thrombolytic Therapy , Thrombosis/etiology , Thrombosis/surgery , Tricuspid Valve/surgery , Female , Humans , Middle Aged , Thrombosis/diagnostic imaging , Thrombosis/drug therapy , Treatment Failure , Tricuspid Valve/diagnostic imaging , UltrasonographyABSTRACT
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