ABSTRACT
Fifteen autosomal STR loci included in the PowerPlex((R))16 System were typed in a population sample of 125 unrelated individuals from Palestinian population of Gaza Strip. Allele frequencies, Hardy-Weinberg equilibrium and forensic parameters were determined for the following loci: Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818.
Subject(s)
Gene Frequency , Genetic Variation , Genetics, Population , Tandem Repeat Sequences , DNA Fingerprinting , Humans , Middle East , Polymerase Chain ReactionABSTRACT
The distribution of allele frequencies and haplotypes for 12 STRs loci, (DYS19, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438 and DYS439) on the Y-chromosome from two Venezuelan populations were determined in 173 DNA samples of unrelated males living in Caracas (62) and Maracaibo (111). Some parameters of forensic importance were calculated. AMOVA and genetic distances between these populations were estimated. The results confirmed Y-STR genotypes as useful markers for forensic genetics analysis.
Subject(s)
Chromosomes, Human, Y/genetics , Forensic Genetics , Genetics, Population , Tandem Repeat Sequences , Ethnicity/genetics , Gene Frequency/genetics , Genetic Markers , Haplotypes , Humans , Male , VenezuelaABSTRACT
Allele frequencies for 15 short tandem repeats (STRs) autosomal loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA, included in the AmpFLSTR Identifiler, Applied Biosystems) were studied in the city of Maracaibo, Venezuela and were compared with other published Latin-American populations for the same loci. Population and forensic parameters were estimated.
Subject(s)
Ethnicity/genetics , Genetic Variation , Genetics, Population , Tandem Repeat Sequences , DNA Fingerprinting , Gene Frequency , Humans , Polymerase Chain Reaction , VenezuelaABSTRACT
Two recently reported short tandem repeat polymorphisms characterized by PCR, D1S1656 and D12S391, were investigated in a sample from Maracaibo, an admixed population of Venezuela, in order to evaluate their application in forensic and population genetics studies. The unbiased heterozygosities were 0.9011 and 0.8444 for locus D1S1656 and D12S391, respectively. The joint discrimination power and joint probability of exclusion were 0.99972 and 0.93287. When allele frequencies of locus D1S1656 from Maracaibo were compared with eight other populations, our group clustered with the European or European-derived samples, mainly from Spain. In the comparison of locus D12S391 with 16 populations, Maracaibo clustered with 3 Asian samples. The high heterozygosity and discrimination power make these two loci important candidates to be considered for STR packages for forensic and population genetic purposes.
Subject(s)
Polymorphism, Genetic , Tandem Repeat Sequences , Alleles , Gene Frequency , Humans , Polymerase Chain Reaction , VenezuelaABSTRACT
Del ADN no codificante del genoma humano, gran parte corresponde a ADN repetitivo en tandem, el cual en los ultimos años ha tenido una extensa aplicación para resolver objetivamente, por encima de los marcadores clásicos, casos de paternidad biológica y de identificación de indivíduos. Una clase particular de este ADN son los loci minisatélites y microsatélites los cuales están constituídos por arreglos de repeticiones en tándem y con una gran variabilidad alélica que los hace mercadores genéticos ideales para esos fines. Este trabajo presenta una revisión sobre estos loci, las técnicas actuales para caracterizarlos, discute su utilidad y planeta algunas consideraciones que deben tenerse en cuenta cuando van a seleccionarse los marcadores para realizar pruebas para determinar paternidad biológica o la identificación de un indivíduo en el campo de la genética forense.
