ABSTRACT
BACKGROUND: Prostate cancer represents 3.8% of cancer deaths worldwide. For most prostate cancer cells to grow, androgens need to bind to a cellular protein called the androgen receptor (AR). This study aims to demonstrate the expression of five microRNAs (miRs) and its influence on the AR formation in patients from the northern region of Brazil. MATERIAL AND METHODS: Eighty-four tissue samples were investigated, including nodular prostatic hyperplasia (NPH) and acinar prostatic adenocarcinoma (CaP). Five miRs (27a-3p, 124, 130a, 488-3p, and 506) were quantified using the TaqMan® Real Time PCR method and AR was measured using Western blotting. RESULTS: Levels of miRs 124, 130a, 488-3p, and 506 were higher in NPH samples. Conversely, in the CaP cases, higher levels of miR 27a-3p and AR were observed. CONCLUSION: In the future, these microRNAs may be tested as markers of CaP at the serum level. The relative expression of AR was 20% higher in patients with prostate cancer, which suggests its potential as a biomarker for prostate malignancy.
Subject(s)
Adenocarcinoma , MicroRNAs , Prostatic Neoplasms , Adenocarcinoma/genetics , Androgens , Cell Line, Tumor , Humans , Male , MicroRNAs/metabolism , Prostate/pathology , Prostatic Neoplasms/pathologyABSTRACT
The presence of human papillomavirus (HPV) was evaluated in 65 samples of prostate tumours and six samples of prostates with benign prostatic hyperplasia from individuals from Northern Brazil. We used a highly sensitive test, the Linear Array HPV Genotyping Test, to detect 37 high and low-risk HPV types. In this study, only 3% of tumour samples showed HPV infection. Our findings support the conclusion that, despite the high incidence of HPV infection in the geographic regions studied, HPV was not associated with a higher risk of prostate cancer. To our knowledge, this is the first study evaluating the frequency of HPV detection in prostatic tissue of individuals from Brazil.
Subject(s)
Papillomaviridae/isolation & purification , Papillomavirus Infections/diagnosis , Prostatic Hyperplasia/virology , Prostatic Neoplasms/virology , DNA, Viral/analysis , Genotype , Humans , Male , Middle Aged , Neoplasm Staging , Papillomaviridae/genetics , Papillomavirus Infections/virology , Polymerase Chain ReactionABSTRACT
The presence of human papillomavirus (HPV) was evaluated in 65 samples of prostate tumours and six samples of prostates with benign prostatic hyperplasia from individuals from Northern Brazil. We used a highly sensitive test, the Linear Array HPV Genotyping Test, to detect 37 high and low-risk HPV types. In this study, only 3 percent of tumour samples showed HPV infection. Our findings support the conclusion that, despite the high incidence of HPV infection in the geographic regions studied, HPV was not associated with a higher risk of prostate cancer. To our knowledge, this is the first study evaluating the frequency of HPV detection in prostatic tissue of individuals from Brazil.
Subject(s)
Humans , Male , Middle Aged , Papillomaviridae/isolation & purification , Papillomavirus Infections/diagnosis , Prostatic Hyperplasia/virology , Prostatic Neoplasms/virology , DNA, Viral/analysis , Genotype , Neoplasm Staging , Polymerase Chain Reaction , Papillomaviridae/genetics , Papillomavirus Infections/virologyABSTRACT
relatar um caso de paciente com diagnóstico de hermafroditismo verdadeiro, abordado através de atendimento multidisciplinar e submetido à mamoplastia bilateral e histerectomia Relato do caso: Homem, 26 anos, com queixa de menúria e mastalgia, sendo identificadas mamas hipertróficas e presença de útero e ovário. Fora submetido à orquiectomia direita há um ano e dois meses, com anatomopatológico identificandoovotéstis. Realizou mamoplastia bilateral e histerectomia, cujo estudo histopatológico da peça cirúrgica evidenciou a presença de tecido ovariano e epididimário, permitindo o diagnóstico de Hermafroditismo Verdadeiro. Considerações finais: O hermafroditismo verdadeiro é uma condição incomum e a maioria destes pacientes não tem acesso a serviços que ofereçam uma abordagem multidisciplinar. No caso descrito,o paciente apresentava menúria e ginecomastia, com genitália externa sem alterações. Possuía diagnóstico histopatológico prévio da presença de ovotéstis. Exames radiológicos revelaram imagens sugestivas de útero e ovário, o que foi confirmado durante o procedimento cirúrgico ao qual foi submetido. A correção cirúrgica, bem como o acompanhamento das diferentes especialidades, lhe proporcionou uma recuperação sem intercorrências, resolução das queixas clínicas e retomada da autoestima
To report the case of a patient with true hermaphroditism, throughmultidisciplinary care and underwent bilateral mammoplasty and hysterectomy. Final considerations: The true hermaphroditism is an uncommon condition and most of these patients have no access to multidisciplinary approach. In the reported case, the patients was admitted with menuria and gynecomastia, and had a normal male external genitalia. He had a histopathological diagnostic of ovotestis and radiological examinations revealed presence of uterus and ovary. The surgical correction and the monitoring of various specialties, it provided an uneventful recovery, resolution of clinical complaints and resumed the self-esteem.
ABSTRACT
Objetivo: relatar um caso em criança de sete anos, apresentando puberdade precoce e submetida à orquiectomia total esquerda por via inguinal, devido dificuldade no diagnóstico preciso do tumor de células de Leydig, durante o ato cirúrgico. Considerações finais: as manifestações endócrinas devem ser levadas em consideração para o diagnóstico diferencial, fazendo com que crianças possam, efetivamente, se beneficiar de cirurgias menos radicais. Após um ano de seguimento não houve recidiva clínica ou hormonal do tumor.
Case Report: the authors report a case of a seven-year-old child, with isosexual precocity, in who had performed a left radical inguinal orchiectomy, due to difficulties in precise diagnosis during surgery. Final Considerations: manifestations of endocrine imbalance could help in diagnosis, permitting further children benefit least radical procedures. No clinical or endocrine relapse after 1 year follow-up.
Subject(s)
Humans , Male , Child , Testicular Neoplasms , Orchiectomy , Leydig Cell TumorABSTRACT
O presente trabalho refere-se a um caso de um paciente portador de cisto de utrículo prostático causando hemospermia, cujo diagnóstico foi elucidado através de exame ultrasonográfico e uretroscopia. O tratamento instituido foi a ressecçäo transuretral
