Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II.

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.

Application of capillary electrophoresis at low pH to oligonucleotides quality control.

A method for oligonucleotides analysis by using capillary electrophoresis at low pH in free solution is described. It may be considered an alternative to classical analytical techniques which use basic buffers and require the presence of sieving media to separate oligonucleotides as a function of their length. On the contrary, at low pH oligo nucleotides can be separated only depending on their base composition. A large set of samples consisting of 72 synthetic oligonucleotides bearing a 5'-alkylamine moiety and designed for HLA genotyping were analysed. The quality of these synthetic oligos was easily assessed, and a single base difference in oligonucleotides of equal sequence was detected. The results suggest the application of this method to the emerging field of mutation detection and single nucleotide polymorfism analysis.

Evaluation of prevalence of "doping" among Italian athletes.

To evaluate knowledge of, attitudes to, and use of illegal drugs and other forms of "doping" in sport 1015 Italian athletes and 216 coaches, doctors, and managers (technicians) were interviewed after selection on a quota basis. Overall, 30% of athletes, managers, and coaches and 21% of doctors indicated that athletic performance can be enhanced by drugs or other doping practices. Over 10% of athletes indicated a frequent use of amphetamines or anabolic steroids at national or international level, fewer athletes mentioning blood doping (7%) and beta-blockers (2%) or other classes of drugs. These proportions were 2-3 times higher for occasional use than for frequent use. Estimates by managers and coaches were much the same as those of athletes when allowance was made for larger random variation. 62% of athletes who acknowledged doping reported pressure to do so from coaches and managers. According to over 70% of athletes access to illegal substances was not difficult. Both athletes and technicians awarded higher scores to risk than to efficacy for any substance, although 42-67% of athletes and technicians regarded amphetamines and anabolic steroids as efficacious. 82% wanted stricter controls not only during competitions but also during training.