ABSTRACT
Five oral strains of Candida albicans and five C. dubliniensis, as well as their respective type-strains, were analyzed by multilocus enzyme electrophoresis (MLEE) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoreses and numerical analyses, we obtained two distinct species-specific taxa, which may justify the use of MLEE and SDS-PAGE as reliable methods for differentiation and complementary identification of C. dubliniensis.
Subject(s)
Candida/classification , Candidiasis, Oral/microbiology , Electrophoresis, Polyacrylamide Gel/methods , Fungal Proteins/analysis , Isoenzymes/analysis , Mouth/microbiology , Mycology/methods , Alcohol Dehydrogenase/analysis , Candida/enzymology , Candida/isolation & purification , Candida albicans/enzymology , Candida albicans/isolation & purification , Catalase/analysis , Glucosephosphate Dehydrogenase/analysis , Humans , Isocitrate Dehydrogenase/analysis , Leucyl Aminopeptidase/analysis , Peroxidase/analysis , Reference Standards , Sodium Dodecyl Sulfate , Species Specificity , Superoxide Dismutase/analysisABSTRACT
Cinco cepas de Candida albicans y otras cinco de C. dubliniensis así como sus cepas-patrón fueron evaluadas mediante el empleo de técnicas de electroforesis en geles de almidón y de poliacrilamida. Después de las electroforesis y evaluación numérica, se obtuvieron dos taxa muy distintos, que pueden justificar el uso de MLEE y SDS-PAGE como métodos confiables para la diferenciación e identificación complementaria de C.dubliniensis. (AU)
Subject(s)
Candida albicans , Electrophoresis, Starch Gel , Electrophoresis, Polyacrylamide Gel , BrazilABSTRACT
Cinco cepas de Candida albicans y otras cinco de C. dubliniensis así como sus cepas-patrón fueron evaluadas mediante el empleo de técnicas de electroforesis en geles de almidón y de poliacrilamida. Después de las electroforesis y evaluación numérica, se obtuvieron dos taxa muy distintos, que pueden justificar el uso de MLEE y SDS-PAGE como métodos confiables para la diferenciación e identificación complementaria de C.dubliniensis.
Subject(s)
Candida albicans , Electrophoresis, Polyacrylamide Gel , Electrophoresis, Starch Gel , BrazilABSTRACT
Five oral strains of Candida albicans and five C. dubliniensis, as well as their respective type-strains, were analyzed by multilocus enzyme electrophoresis (MLEE) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoreses and numerical analyses, we obtained two distinct species-specific taxa, which may justify the use of MLEE and SDS-PAGE as reliable methods for differentiation and complementary identification of C. dubliniensis.
ABSTRACT
The aim of this study was to quantify the microorganisms Streptococcus mutans and Candida sp in the oral cavity of patients with oropharynx carcinoma, before, during and after radiotherapy, and to correlate the results with salivary factors such as pH, buffer capacity (CT) and flow rate (FS). Saliva samples were collected, diluted and inoculated in SB-20 agar and in Sabouraud agar, for Streptococcus mutans and Candida sp, respectively. Previously to dilution, the concentrated saliva was analyzed, and the salivary factors were determined. After the growth of colonies, the number of microorganisms was determined in CFU/ml. The analysis of the results allowed to conclude that the salivary factors are related to the presence of microorganisms, and that the number of CFU/ml increased as salivary flow rate decreased. The effects of radiation compromised salivary homeostasis and favored the increase of infection by yeasts and bacteria.
