ABSTRACT
To new triterpenes, trichomycins A (1) and B (2), were purified from the new species Tricholoma sp. AU1 by activity-guided fractionation following their antibacterial activity. The two compounds were found to have a hitherto unreported triterpenoid skeleton. The structures and relative stereochemistry of 1 and 2 were determined through extensive 2D NMR spectroscopy, while the inhibitory activity of 1 and 2 against two Gram-positive and two Gram-negative bacteria and a mammalian cell line was determined.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Basidiomycota/chemistry , Triterpenes/isolation & purification , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Australia , Drug Screening Assays, Antitumor , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Microbial Sensitivity Tests , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Triterpenes/chemistry , Triterpenes/pharmacology , Tumor Cells, CulturedABSTRACT
Chemical investigations of the crude MeOH extract of Physalis viscosa led to the identification of the novel acylated sucrose ester physaloside A (1). The structure of 1 was determined by 2D NMR analysis, and the absolute configuration was determined by chemical degradation and comparison with authentic standards.
