ABSTRACT
Principal neurons of the medial nucleus of the trapezoid body (MNTB) integrate the large, excitatory inputs from anteroventral cochlear nucleus (AVCN) bushy cells with conventional inhibitory inputs to produce an inhibitory output to the lateral and medial superior olive. This circuit is critical in the sound localization pathway of the auditory brainstem. Many ionic currents act in concert to produce the rapid phase-locked firing properties characteristic of MNTB principal neurons. We report here that MNTB neurons of the mouse possess a 2-4nS instantaneous potassium-based leak current, probably mediated by TWIK two-pore potassium leak channels. The function of the leak current was examined by modulating its magnitude with a dynamic clamp. The leak current modulates the resting voltage by 5mV/nS, reduces the input resistance of the cell by 5MOmega/nS and reduces the membrane time constant by 0.075 micros/nS. The leak current also modulates spike timing. Given leak channels are highly regulated, they are well placed to influence the firing properties, and action potential timing in principal neurons of the MNTB.
