ABSTRACT
The passage of hepatitis C virus (HCV) across the dialysis membrane is a controversial issue. We performed a study applying extreme conditions of permeability to the dialysis membrane and avoiding the use of heparin and dialysis bath that might interfere with polymerase chain reaction (PCR) results. We obtained samples from the ultrafiltrate at the beginning of 18 hemodialysis sessions carried out in 6 HCV RNA-positive patients. HCV RNA was detected by PCR in 3 (16.7%) ultrafiltrate samples belonging to 1 of the patients. HCV genotype was the same as that found in positive ultrafiltrate samples and in the serum corresponding to this patient. The viral load of this patient was under the levels detectable by the assay employed. Therefore, contamination of the ultrafiltrate may constitute a potential risk for HCV transmission in hemodialysis units.
Subject(s)
Hepacivirus/isolation & purification , Hepatitis C/transmission , Membranes, Artificial , Renal Dialysis/instrumentation , Adult , Artifacts , Female , Humans , Male , Middle Aged , Polymerase Chain Reaction , Ultrafiltration , Viral LoadABSTRACT
Conclusiones: Nuestra experiencia inicial con DPI resultó satisfactoria. Los resultados dependen fuertemente de la selección del paciente. Los resultados con DPCA son alentadores. Es preciso trabajar con materiales confiables. Se estima que una atinada selección de los candidatos es necesaria. (AU)
Subject(s)
Humans , Peritoneal Dialysis/statistics & numerical data , Renal Insufficiency, Chronic/therapy , Peritoneal Dialysis/adverse effects , Peritoneal Dialysis/mortalityABSTRACT
Conclusiones: Nuestra experiencia inicial con DPI resultó satisfactoria. Los resultados dependen fuertemente de la selección del paciente. Los resultados con DPCA son alentadores. Es preciso trabajar con materiales confiables. Se estima que una atinada selección de los candidatos es necesaria.
