1.
J Immunol Methods
; 164(1): 21-5, 1993 Aug 26.
Article
in English
| MEDLINE
| ID: mdl-8360506
ABSTRACT
We describe a new flow cytometric B cell crossmatch method with improved sensitivity and specificity. It is based on the coating of B cell surface immunoglobulins with an unconjugated polyvalent anti-human immunoglobulin antibody. The method also provides a means for determining the specificity of anti-B cell antibodies and, potentially, for anti-HLA class II antibody specificity differentiation (DR, DQ or DP) in a binding inhibition test using mouse monoclonal antibodies directed against human HLA class II antigens.
Subject(s)
B-Lymphocytes/immunology , Histocompatibility Testing/instrumentation , Isoantibodies/analysis , Antibody Specificity , Flow Cytometry/methods , HLA-D Antigens/immunology , Humans
2.
Transplant Proc
; 22(4): 1885-6, 1990 Aug.
Article
in English
| MEDLINE
| ID: mdl-2389472
3.
Transplant Proc
; 22(4): 1895-6, 1990 Aug.
Article
in English
| MEDLINE
| ID: mdl-2389477
4.
Transplant Proc
; 21(1 Pt 1): 702-3, 1989 Feb.
Article
in English
| MEDLINE
| ID: mdl-2650231