ABSTRACT
Endometrial cancer (EC) is a significant cause of cancer-related deaths in women. MicroRNAs (miRs) play a role in cancer development, acting as oncogenes or tumor suppressors. This study evaluated the diagnostic potential of hsa-miR-185-5p and hsa-miR-191-5p in EC and their correlation with clinical and histopathological features. A cross-sectional study analyzed formalin-fixed, paraffin-embedded tissue samples from 59 patients: 18 with EC, 21 with endometrial hyperplasia (EH), 17 with normal endometrium (NE), and 3 with endometrial polyps (EPs). Quantitative reverse transcription-polymerase chain reaction and TaqMan probes were used for miR expression analysis. The Shapiro-Wilk test was used to analyze the normal distribution of the data. Subsequently, parametric or non-parametric tests were used to evaluate the associations between the expression levels of each miR and clinical parameters. Both miRs were underexpressed in some precursor and malignant lesions compared to certain NE subtypes and benign lesions. Specifically, hsa-miR-185-5p showed underexpression in grade 3 EC compared to some NE and EH subtypes (FC: -57.9 to -8.5, p < 0.05), and hsa-miR-191-5p was underexpressed in EH and EC compared to secretory endometrium and EPs (FC: -4.2 to -32.8, p < 0.05). SETD1B, TJP1, and MSI1 were common predicted target genes. In conclusion, hsa-miR-185-5p and hsa-miR-191-5p are underexpressed in EC tissues, correlating with histopathological grades, highlighting their potential as diagnostic biomarkers and their role as tumor suppressors in EC.
Subject(s)
Endometrial Neoplasms , Endometrium , Gene Expression Regulation, Neoplastic , MicroRNAs , Humans , Female , MicroRNAs/genetics , MicroRNAs/metabolism , Endometrial Neoplasms/genetics , Endometrial Neoplasms/pathology , Endometrial Neoplasms/metabolism , Endometrium/metabolism , Endometrium/pathology , Middle Aged , Cross-Sectional Studies , Neoplasm Grading , Adult , Aged , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolismABSTRACT
In developed countries, endometrial cancer (EC) is one of the most common neoplasms of the female reproductive system. MicroRNAs (miRs) are a class of single-stranded noncoding RNA molecules with lengths of 19-25 nucleotides that bind to target messenger RNA (mRNA) to regulate post-transcriptional gene expression. Although there is a large amount of research focused on identifying miRs with a diagnostic, prognostic, or response to treatment capacity in EC, these studies differ in terms of experimental methodology, types of samples used, selection criteria, and results obtained. Hence, there is a large amount of heterogeneous information that makes it difficult to identify potential miR biomarkers. We aimed to summarize the current knowledge on miRs that have been shown to be the most suitable potential markers for EC. We searched PubMed and Google Scholar without date restrictions or filters. We described 138 miRs with potential diagnostic, prognostic, or treatment response potential in EC. Seven diagnostic panels showed higher sensitivity and specificity for the diagnosis of EC than individual miRs. We further identified miRs up- or downregulated depending on the FIGO stage, precursor lesions, and staging after surgery, which provides insight into which miRs are expressed chronologically depending on the disease stage and/or that are modulated depending on the tumor grade based on histopathological evaluation.
ABSTRACT
BACKGROUND: Splenic cystic lesions are rare. They are classified as type 1 with an epithelial component and type 2 (pseudocysts) without an epithelial component. Pseudocysts are the result of trauma, heart attacks, or infection. In Mexico there are no cases reported in pregnancy. CASE REPORT: 26-year-old woman, pregnant, asymptomatic. With a spleen-dependent cystic intraparenchymal lesion measuring 25 × 18 × 10 cm. Undergoing open splenectomy in the second trimester of pregnancy with satisfactory evolution. Histological analysis demonstrated a splenic pseudocyst. We describe the first case of splenic pseudocyst in a pregnant patient in Mexico.
ANTECEDENTES: Las lesiones quísticas esplénicas son infrecuentes. Se clasifican en tipo 1, con componente epitelial, y en tipo 2 (pseudoquistes), sin componente epitelial. Los pseudoquistes son resultado de traumatismos, infartos o infección. En México no existe ningún caso reportado en el embarazo. CASO CLÍNICO: Mujer de 26 años, embarazada, asintomática. Presenta una lesión intraparenquimatosa quística dependiente del bazo, de 25 × 18 × 10 cm. Es sometida a esplenectomía abierta en el segundo trimestre del embarazo, con evolución satisfactoria. El análisis histológico demostró un pseudoquiste esplénico. Describimos el primer caso de pseudoquiste esplénico en una paciente embarazada en México.
