The relationship between zooplankton vertical distribution and the concentration of aqueous Hg in boreal lakes: A comparative field study.

The production of the highly toxic monomethylmercury (MeHg) is heterogenous throughout the water column. Multiple factors have been identified to significantly affect this process, such as an extended anoxic water layer and a deep-water phytoplankton maximum. However, the role of water column heterogeneity on mercury (Hg) cycling is still poorly known, especially concerning the role of zooplankton grazers. Here, four boreal lakes with contrasting characteristics were sampled (i.e., transparency and the presence/absence of fish) at both day and night in order to maximize the heterogeneity in zooplankton abundance both among and within lakes, and to investigate their potential links with Hg vertical heterogeneity. Diel variation of the concentrations of both dissolved total Hg (DTHg) and total Hg (THg) were observed, with night samples significantly higher than day samples. Although this pattern was not related to diel changes in the vertical distribution of zooplankton, results showed that the presence of large copepods (>1.2 mm) and medium-sized (0.6 to 1.2 mm) cladocerans was significantly associated with lower concentrations of DTHg in the water at a given depth, whereas the presence of medium-sized copepods was significantly associated with the concentration of THg. The presence of cladocerans was significantly associated with the ratio between the dissolved MeHg and DTHg (conventionally used as a proxy of methylation potential). Phytoplankton biomass was directly correlated with the concentration of both dissolved and total MeHg and the methylation potential. At the same time, phytoplankton biomass was inversely related to the fraction of DTHg. These results suggest a potential key role of the heterogeneity of biotic factors in the water column, especially of phytoplankton and zooplankton, in the cycling of total Hg and MeHg in boreal lakes.

Grazer-mediated regeneration of methylmercury, inorganic mercury, and other metals in freshwater.

Whereas it is well established that zooplankton can transfer various pollutants such as mercury (Hg) from primary producers to higher trophic levels, less is known on the effects of their activities on the recycling of Hg in aquatic ecosystems. Here, the impact of zooplankton grazing efficiency and excretion/egestion processes on metal concentrations in freshwater was investigated. Isotopically labeled algae (200HgCl2, Me198HgCl) was used as a food source and the transfer of the selected isotopes to the culture medium during grazing was measured. In parallel, the potential for the recycling of major ions and metals, including selected essential and non-essential metals, was investigated for this could help to track the effects of sloppy feeding. To highlight the role of feeding behavior, a large filter-feeder (Daphnia magna) was compared to a smaller selective feeder zooplankton taxon (calanoid copepods), with the latter being expected to cause more sloppy feeding than the former. The experiments demonstrated that zooplankton grazing of both taxa significantly influenced the concentrations of the particulate portion of both inorganic Hg (IHg) and monomethylmercury (MeHg) in water. In contrast, only Daphnia significantly increased the concentration of dissolved IHg, whereas the concentration of dissolved MeHg was not affected by either grazer. The results also suggested that both taxa affected the concentrations of dissolved Fe, Zn, SO42- and rare earth elements via sloppy feeding, whereas only Daphnia significantly increased the concentration of dissolved Cu via this mechanism. The effects of excretion/egestion were negligible except for dissolved IHg and Cu in Daphnia treatment. These results highlight a neglected pathway of IHg and MeHg recycling in the water column in freshwater ecosystems with potentially important consequences for trophic transfer.

Linking fisheries to land use: How anthropogenic inputs from the watershed shape fish habitat quality.

Aquatic ecosystems are increasingly threatened by anthropogenic stressors, both at local and larger scales. For instance, runoff from intensively cultivated areas leads to higher nutrient and sediment concentrations deteriorating water quality, which potentially trigger trophic state changes. Unfortunately, we have a poor understanding of the complex relationships linking water quality degradation and different ecosystem components. Here we analyze the long-term cascading effects of several anthropogenic stressors on both submerged aquatic vegetation (SAV) and the key traits of an exploited yellow perch (Perca flavescens, YP) population from the watershed of Lake Saint-Pierre - the largest fluvial lake of the St. Lawrence River (Québec, Canada). Lake Saint-Pierre drains one of the most impacted watersheds in Eastern Canada and had sustained a YP fishery (worth up to 10 M$ CAN/year) until the population collapsed in the mid-1990s. SAV abundance has declined since the 1980s, partially overlapping with the YP collapse. Within a structural equation modeling framework, we tested the links between changes in both SAV abundance and the YP fishery with abiotic stressors acting at both local and larger scales. Our results show that both SAV and YP declines are causally associated with anthropogenic nutrient and sediment loadings from the watershed. The decline of YP landings is also explained by a reduction in SAV abundance and YP juvenile growth, mainly caused by a sharp decrease in water transparency over the last decades. These results suggest a causal association between environmental degradation due to nutrients and sediments and different components of the trophic aquatic network. Such an integrative approach is crucial for the development of management strategies that consider cultivated lands and aquatic systems as a continuum rather than separate compartments. SAV restoration is thus a critical feature contributing to water depuration and promoting the recovery of fish populations threatened by habitat degradation.

Alternative host identity and lake morphometry drive trematode transmission in brook charr.

Both biotic and abiotic factors have been invoked to explain the large variations observed in the prevalence and abundance of parasites in aquatic ecosystems. However, we have only a poor knowledge of the potential interplay among these factors in natural systems. It is, therefore, important to analyze the effects of multiple potential environmental drivers together to get an integrated view of their influence on the prevalence and abundance of parasites. To this end, we selected two genera of digenean trematode parasites that require at least two hosts to complete their life cycle and use two different transmission strategies. Crepidostomum moves through a trophic pathway via consumption of infected prey by the host, while Apophallus infects its hosts via direct penetration of their skin. This study was conducted in 23 Canadian Shield lakes exhibiting orthogonal gradients of biotic (fish species richness and biomass) and abiotic (morphometry, physico-chemical) variables. We quantified prevalence and abundance of these parasites in the skin and intestine of brook charr (Salvelinus fontinalis). Our results show that biotic factors are key drivers of parasite abundance and prevalence, with Apophallus being negatively associated with the fish species richness-biomass gradient, and Crepidostomum responding more to identity of host than to the diversity gradient. Among the abiotic variables, lake area was found to be positively related to both prevalence and abundance in Apophallus. Our results suggest that taking into account the interplay of both biotic and abiotic factors is crucial for understanding patterns of parasite transmission success in boreal lakes.

Interactive effects of neonicotinoids and natural ultraviolet radiation on yellow perch (Perca flavescens) larvae.

Neonicotinoids (NEOCs) are insecticides that are widely used worldwide in the culture of maize and soya. Whereas they specifically target terrestrial insects by acting as agonists of the neurotransmitter acetylcholine in their nervous system, their effects on the cholinergic system of vertebrates is still unclear. Moreover, there is an increasing concern about their effects on aquatic biota because of their high leaching potential. In the agricultural watershed of Lake St. Pierre (LSP) (St. Lawrence River System, Québec, Canada), for example, NEOC concentrations considered toxic for aquatic biota (>8.3 ng L-1) have frequently been detected. These conditions may affect the yellow perch (Perca flavescens) population in LSP, which collapsed in the mid 1990s and is now experiencing poor recruitment. Moreover, because their larvae are found in shallow waters (<80 cm) near agricultural land, they are also exposed to ultraviolet radiation (UVR), with unknown potential interactions with NEOCs. The objective of this study was to test the synergistic effects of two commonly used NEOCs (imidacloprid and thiamethoxam) with natural UVR on yellow perch larvae using survival analysis and biomarkers to better quantify lethal and sublethal effects. Three common garden experiments were conducted with thiamethoxam and/or imidacloprid and natural UVR following a factorial design. Our results showed an interaction between UVR and thiamethoxam in terms of larval mortality. At the sublethal level, imidacloprid was associated with increased protein content and, in the presence of UVR, with increased acetylcholinesterase activity, thus indicating a cholinergic perturbation like that found in insects. Finally, we also found unexpected reduced lipid peroxidation associated with imidacloprid. A reduction in the overall lipid accumulation is suspected to be behind this puzzling result. These results will open new research avenues related to the effects of NEOCs on proteins and lipid accumulation.

Measurements of Night Sky Brightness in the Veneto Region of Italy: Sky Quality Meter Network Results and Differential Photometry by Digital Single Lens Reflex.

In this paper, we present the implementation of a monitoring network for artificial light at night (ALAN), based on Sky Quality Meter devices (SQM) installed in seven locations of the Veneto region. The system is coordinated by the Regional Environmental Protection Agency (ARPA-Veneto) and the Department of Physics and Astronomy of the University of Padova, in collaboration with a local dark-sky association, Venetostellato. A new centralized database containing zenith night sky brightness (NSB) data was implemented to collect data from all SQM stations of the regional territory, not only in real time (since 2017), but in some stations since 2011. We now have a dataset to determine how light pollution is affecting astronomical observatories. A WEB portal was created to offer different downloads from these NSB data. We present the results of some elaborations for the 2018 dataset (statistics, histograms, annual and cumulative plots) for seven monitoring sites. For Ekar and Pennar sites, we also present the NSB monthly trend from 2014 until the time of the study. We purchased a reflex camera with a fish eye lens, appropriately calibrated with the software (SW) Sky Quality Camera, which allowed us to study ALAN using differential photometry. Here, we present our first results obtained by studying the night evolution of light pollution in the urban location of Padova.

Effects of lake warming on behavioural thermoregulatory tactics in a cold-water stenothermic fish.

Despite some evidence of within-population phenotypic variation in fish thermal behaviour, the occurrence of alternative tactics of this behaviour is rarely explicitly considered when studying natural populations. Brook charr provide an example of within-population variability in behavioural thermoregulation as revealed by a recent study on a lacustrine population of this species. The objectives of the present study were (i) to determine the influence of natural variability in the lake's thermal profiles on the expression of thermoregulatory tactics, and (ii) to determine the vertical and horizontal movements of individuals at different periods of the day to better understand the spatio-temporal behaviour associated with each thermoregulatory tactic. During summer 2010, 30 adult brook charr were equipped with thermo-sensitive radio transmitters to monitor their selected temperatures and daily movements. These individuals exhibited the same four behavioural thermoregulatory tactics observed in 2003 and 2005, but the expression of two of these was weaker in 2010. This result was associated with lake warming, which constrained the expression of two thermoregulatory tactics: brook charr significantly decreased their selected temperatures and daily movements when the mean daily epilimnion temperature was above 22.4°C. This study shows for the first time that the expression of behavioural thermoregulatory tactics is related to the lake's thermal regime and that the tactics are plastic through time.

Inferring processes from spatial patterns: the role of directional and non-directional forces in shaping fish larvae distribution in a freshwater lake system.

Larval dispersal is a crucial factor for fish recruitment. For fishes with relatively small-bodied larvae, drift has the potential to play a more important role than active habitat selection in determining larval dispersal; therefore, we expect small-bodied fish larvae to be poorly associated with habitat characteristics. To test this hypothesis, we used as model yellow perch (Perca flavescens), whose larvae are among the smallest among freshwater temperate fishes. Thus, we analysed the habitat association of yellow perch larvae at multiple spatial scales in a large shallow fluvial lake by explicitly modelling directional (e.g. due to water currents) and non-directional (e.g. due to aggregation) spatial patterns. This allowed us to indirectly assess the relative roles of drift (directional process) and potential habitat choice on larval dispersal. Our results give weak support to the drift hypothesis, whereas yellow perch show a strong habitat association at unexpectedly small sizes, when compared to other systems. We found consistent non-directional patterns in larvae distributions at both broad and medium spatial scales but only few significant directional components. The environmental variables alone (e.g. vegetation) generally explained a significant and biologically relevant fraction of the variation in fish larvae distribution data. These results suggest that (i) drift plays a minor role in this shallow system, (ii) larvae display spatial patterns that only partially covary with environmental variables, and (iii) larvae are associated to specific habitats. By suggesting that habitat association potentially includes an active choice component for yellow perch larvae, our results shed new light on the ecology of freshwater fish larvae and should help in building more realistic recruitment models.

Behavioural thermoregulatory tactics in lacustrine brook charr, Salvelinus fontinalis.

The need to vary body temperature to optimize physiological processes can lead to thermoregulatory behaviours, particularly in ectotherms. Despite some evidence of within-population phenotypic variation in thermal behaviour, the occurrence of alternative tactics of this behaviour is rarely explicitly considered when studying natural populations. The main objective of this study was to determine whether different thermal tactics exist among individuals of the same population. We studied the behavioural thermoregulation of 33 adult brook charr in a stratified lake using thermo-sensitive radio transmitters that measured hourly individual temperature over one month. The observed behavioural thermoregulatory patterns were consistent between years and suggest the existence of four tactics: two "warm" tactics with both crepuscular and finer periodicities, with or without a diel periodicity, and two "cool" tactics, with or without a diel periodicity. Telemetry data support the above findings by showing that the different tactics are associated with different patterns of diel horizontal movements. Taken together, our results show a clear spatio-temporal segregation of individuals displaying different tactics, suggesting a reduction of niche overlap. To our knowledge, this is the first study showing the presence of behavioural thermoregulatory tactics in a vertebrate.

Predicting shifts in dynamics of cannibalistic field populations using individual-based models.

The occurrence of qualitative shifts in population dynamical regimes has long been the focus of population biologists. Nonlinear ecological models predict that these shifts in dynamical regimes may occur as a result of parameter shifts, but unambiguous empirical evidence is largely restricted to laboratory populations. We used an individual-based modelling approach to predict dynamical shifts in field fish populations where the capacity to cannibalize differed between species. Model-generated individual growth trajectories that reflect different population dynamics were confronted with empirically observed growth trajectories, showing that our ordering and quantitative estimates of the different cannibalistic species in terms of life-history characteristics led to correct qualitative predictions of their dynamics.

Scaling food chains in aquatic mesocosms: do the effects of depth override the effects of planktivory?

To assess the effects of physical dimension and planktivorous fish on phytoplankton standing crop, we repeated an experiment at different scales in plastic enclosures during summer 1995 in Lake Créteil, France. Enclosures were scaled for a constant surface (1.5 × 1.5 m) as depth was increased from 2.5 to 4.5 m. Even-link (zooplankton and phytoplankton) and odd-link (planktivorous fish, zooplankton and phytoplankton) food webs were established in both shallow and deep enclosures. Fish densities in the deep enclosures were scaled to allow comparisons with shallow ones for both in individuals m-2 or individuals m-3. We explicitly designed this experiment to examine the scale-dependent behavior of the top-down mechanism of algal biomass control in lakes, and in particular to test the hypothesis of stronger cascading effects of fish on lower trophic levels at reduced depth. Both fish and enclosure size had highly significant effects on phytoplankton biomass over the duration of the experiment. No depth × fish interaction effects were observed. The presence of planktivorous fish enhanced phytoplankton biomass in both shallow and deep enclosures, although the reduction in depth generally produced a stronger effect. The mean concentration of chlorophyll a in the deep odd-link systems (ca 5 mg m-3) was lower than in the shallow even-link systems (ca 17 mg m-3). Statistical interpretation did not change when data were expressed as phytoplankton biomass per unit of surface area. Light limitation and zooplankton grazing are the most probable mechanisms explaining our results in these nutrient-enriched systems. Moreover, we found that the strength of the cascading effect of fish on plankton was not a function of depth. We believe that further studies on scaling effects should be conducted in order to improve our understanding of ecological patterns and to extrapolate results from micro/mesocosms to natural ecosystems.