ABSTRACT
BACKGROUND AND OBJECTIVES: Detection of anti-platelet antibodies is required for the diagnosis of foetal/neonatal alloimmune thrombocytopaenia. The most commonly used methods for anti-platelet antibody detection are the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) and the Luminex bead assay (PakLx). However, for economic reasons, the use of the PakLx assay is limited. MATERIALS AND METHODS: In the present study, we evaluated the performance of an optimized protocol based on a half-volume of PakLx reagents. We compared two alternative procedures: one with a half-volume of all components including patient samples, and another based on a half-volume of reagents but a standard volume of patient sample. RESULTS: Our results obtained with a panel of 67 samples demonstrate improved sensitivity when using a standard sample volume. CONCLUSION: In the event of an inconclusive result with this optimized protocol (e.g., incomplete panel of positive Luminex beads), we recommend testing the sample with an alternative protocol (e.g., MAIPA or the original PakLx protocol).
Subject(s)
Antigens, Human Platelet , Infant, Newborn , Humans , Cost-Benefit Analysis , Isoantibodies , Immunologic Tests , Antibodies, Monoclonal , Blood PlateletsABSTRACT
DQA1*05:18 differs from DQA1*05:01:01:03 by one nucleotide substitution at position 94 in exon 2.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , HLA-DQ alpha-Chains/genetics , Humans , Sequence Analysis, DNAABSTRACT
DQA1*03:11 differs from DQA1*03:03:01:01 by one nucleotide substitution at position 664 in exon 4.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , HLA-DQ alpha-Chains/genetics , Humans , Sequence Analysis, DNAABSTRACT
DQA1*01:39 differs from DQA1*01:02:01:01 by one nucleotide substitution at position 49 in exon 1.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , HLA-DQ alpha-Chains/genetics , Humans , Sequence Analysis, DNAABSTRACT
B*51:296 differs from B*51:01:01:01 by one nucleotide substitution at position 1030 in exon 6.
Subject(s)
HLA-B Antigens , High-Throughput Nucleotide Sequencing , Alleles , Exons/genetics , HLA-B Antigens/genetics , Humans , Mutation, MissenseABSTRACT
B*40:450 differs from B*40:01:02:01 by one nucleotide substitution at position 5 in exon 1.
Subject(s)
Genes, MHC Class I , HLA-B Antigens , Alleles , Exons/genetics , HLA-B Antigens/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
B*44:452 differs from B*44:02:01:01 by one nucleotide substitution at position 527 in exon 3.
Subject(s)
Genes, MHC Class I , HLA-B Antigens , Alleles , Exons/genetics , HLA-B Antigens/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
DQB1*03:417 differs from DQB1*03:01:01:01 by one nucleotide substitution at position 179 in exon 2.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , Exons/genetics , HLA-DQ beta-Chains/genetics , HumansABSTRACT
B*44:476 differs from B*44:03:01:01 by one nucleotide substitution at position 934 in exon 5.
Subject(s)
Genes, MHC Class I , HLA-B Antigens , Alleles , Exons/genetics , HLA-B Antigens/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
DRB1*01:107 differs from DRB1*01:01:01:01 by one nucleotide substitution at position 484 in exon 3.
Subject(s)
HLA-DRB1 Chains , Alleles , Exons/genetics , HLA-DRB1 Chains/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
DRB1*11:260 differs from DRB1*11:03:01 by one nucleotide substitution at position 484 in exon 3.
Subject(s)
HLA-DRB1 Chains , Alleles , Exons/genetics , HLA-DRB1 Chains/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
A*24:470 differs from A*24:02:01:01 by one nucleotide substitution at position 46 in exon 1.
Subject(s)
High-Throughput Nucleotide Sequencing , Nucleotides , Alleles , Exons/genetics , HLA-A Antigens , HumansABSTRACT
B*15:474 differs from B*15:01:01:01 by one nucleotide substitution at position 923 in exon 5.
Subject(s)
Genes, MHC Class I , HLA-B Antigens , Alleles , Exons/genetics , HLA-B Antigens/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
DQB1*03:400N differs from DQB1*03:01:01:03 by one nucleotide insertion at position 535 in exon 3.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , Exons/genetics , HLA-DQ beta-Chains/genetics , HumansABSTRACT
DQB1*06:352 differs from DQB1*06:03:01:01 by one nucleotide substitution at position 668 in exon 4.
Subject(s)
High-Throughput Nucleotide Sequencing , Alleles , Exons/genetics , HLA-DQ beta-Chains/genetics , HumansABSTRACT
A*32:134 differs from A*32:01:01:01 by one nucleotide substitution at position 925 in exon 5.
Subject(s)
High-Throughput Nucleotide Sequencing , Nucleotides , Alleles , Exons/genetics , HLA-A Antigens/genetics , HumansABSTRACT
A*11:349 differs from A*11:01:01:01 by one nucleotide substitution at position 688 in exon 4.
