ABSTRACT
The oral medicinal form of house-dust allergen has been prepared with the use of new technology. To produce the preparation, the standard water-saline extract obtained from house-dust mites (Dermatophagoides farinae) allergen has been used. This preparation is chemically stable and does not lose its biological activity for 2 - 3 years of storage. The microbiological purity of the allergen meets the requirements for oral preparations. The allergen has proved to be safe in cases of prolonged administration and has no chronic toxicity. As shown with the use of immunochemical methods, the preparation has high immunogenicity and lower allergenicity. This preparation may be recommended for clinical trials on a limited circle of subjects.
Subject(s)
Allergens/administration & dosage , Dermatophagoides farinae/immunology , Hypersensitivity/therapy , Immunotherapy , Tissue Extracts/administration & dosage , Administration, Oral , Allergens/adverse effects , Allergens/immunology , Animals , Antibody Specificity , Dermatophagoides farinae/chemistry , Dose-Response Relationship, Immunologic , Drug Evaluation, Preclinical , Guinea Pigs , Immunoglobulin G/immunology , Mice , Proteins/administration & dosage , Proteins/adverse effects , Proteins/immunology , Tissue Extracts/adverse effects , Tissue Extracts/immunologyABSTRACT
Physical, chemical and immunobiological characteristics of allergens from synanthropic insects were studied by tests for anaphylaxis, indirect degranulation of mast cells test and ELISA. Sera from 20 patients with severe and intermediate atopic asthma with sensiblization to common allergens have been studied. All extracts of allergens from synanthropic insects (german cockroach, oriental cockroach, american cockroach, speckled feeder cockroach, cricket, common house fly, brown house moth, confused flour beetle, rice weevil, grain weevil) have specific activity. Extracts of allergens from common house fly, brown house moth, german cockroach and oriental cockroach had the strongest allergenic activity as measured by ELISA. Obtained allergens can be used for insect allergy diagnostics.
Subject(s)
Allergens/adverse effects , Allergens/immunology , Insect Proteins/adverse effects , Insect Proteins/immunology , Insecta/immunology , Allergens/isolation & purification , Anaphylaxis/chemically induced , Animals , Antibody Specificity , Asthma/blood , Cell Degranulation/immunology , Guinea Pigs , Humans , Hypersensitivity/diagnosis , Immunoglobulin E/blood , Immunoglobulin E/immunology , Insect Proteins/isolation & purification , Male , Mast Cells/immunology , RatsABSTRACT
Characteristics of allergy to mites in children living in Tula region have been revealed. It was shown that mites from Pyroglyphidae (Dermatophagoides pteronyssinus, Dermatophagoides farinae), Cheyletidae and Glycyphagidae (G. destructor, G. domesticus) families play important role in development of atopic allergy in children from this region. Efficacy of plant-origin acaricide "Milbiol" as part of prophylactic measures was evaluated. Its use in children with mild and intermediate asthma led to decrease of number of wheezing episodes, improvement of respiratory function, lessening of clinical signs of allergic rhinitis, decrease or discontinuation of usage of vasoconstrictive preparations.
Subject(s)
Allergens/adverse effects , Asthma/prevention & control , Mites/immunology , Allergens/analysis , Animals , Asthma/etiology , Child , Environmental Monitoring , Epidemiological Monitoring , Female , Humans , Male , Mites/classification , Pesticides , Russia/epidemiologyABSTRACT
Different methods of obtaining allergens from vegetables, fruit and berries have been evaluated. Two technologies for obtaining food allergens have been considered; of these, Coca's method has proved to be more technological, economical and less labor-consuming. The newly developed technology has made it possible to obtain stable, safe and specifically active allergens which may be used for diagnosing food allergy.
