ABSTRACT
Rotaviruses are segmented double-stranded RNA viruses that cause gastroenteritis in mammals and birds. Here we describe the first partial nucleotide sequences of the structural protein VP6 from the genomes of group F rotaviruses that were detected in 5 out of 53 fecal samples (9.43%) from healthy broilers from Brazilian poultry farms based on reverse-transcriptase-PCR with primers designed for this study. The findings support the development of molecular detection systems, which can be used for the assessment of the distribution of rotavirus F in birds, their potential involvement in diseases, and their impact on poultry health.
Subject(s)
Chickens/virology , Disease Reservoirs/virology , Rotavirus/isolation & purification , Amino Acid Sequence , Animal Husbandry/instrumentation , Animals , Brazil , Feces/virology , Molecular Sequence Data , Phylogeny , Rotavirus/classification , Rotavirus/genetics , Sequence Alignment , Viral Proteins/chemistry , Viral Proteins/geneticsABSTRACT
Rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. Using different PCR protocols, we report the occurrence of rotavirus A in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from Brazilian avian farms. We typed the G5, G8, G11, G19, and P[31] genotypes.
