ABSTRACT
Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates. Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets. A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments. The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS. The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay. The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains. The surface roughness has progressively increased with chitosan concentration. FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan. The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan. The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
Subject(s)
Anti-Infective Agents/chemistry , Apatites/chemistry , Biomimetic Materials/chemistry , Chitosan/chemistry , Escherichia coli/drug effects , Staphylococcus aureus/drug effects , Anti-Infective Agents/pharmacology , Apatites/pharmacology , Biomimetic Materials/pharmacology , Chitosan/pharmacology , Powders , Spectroscopy, Fourier Transform Infrared/methodsABSTRACT
This work reports on the unprecedented magnetron sputtering deposition of submicrometric hollow cones of bioactive glass at low temperature in the absence of any template or catalyst. The influence of sputtering conditions on the formation and development of bioglass cones was studied. It was shown that larger populations of well-developed cones could be achieved by increasing the argon sputtering pressure. A mechanism describing the growth of bioglass hollow cones is presented, offering the links for process control and reproducibility of the cone features. The composition, structure, and morphology of the as-synthesized hollow cones were investigated by energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), grazing incidence geometry X-ray diffraction (GIXRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM)-selected area electron diffraction (SAED). The in vitro biological performance, assessed by degradation tests (ISO 10993-14) and cytocompatibility assays (ISO 10993-5) in endothelial cell cultures, was excellent. This allied with resorbability and the unique morphological features make the submicrometer hollow cones interesting candidate material devices for focal transitory permeabilization of the blood-brain barrier in the treatment of carcinoma and neurodegenerative disorders.
Subject(s)
Carcinoma/drug therapy , Ceramics/chemical synthesis , Coated Materials, Biocompatible/chemical synthesis , Endothelial Cells/drug effects , Argon/chemistry , Carcinoma/pathology , Cell Line , Ceramics/chemistry , Ceramics/pharmacology , Coated Materials, Biocompatible/chemistry , Coated Materials, Biocompatible/pharmacology , Endothelial Cells/ultrastructure , Humans , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Spectroscopy, Fourier Transform Infrared , Titanium/chemistry , X-Ray DiffractionABSTRACT
In this paper we present our experience concerning 658 excimer laser operations: statistical data about the number of operations, preoperative investigations, postoperative care and postoperative complications.
Subject(s)
Keratomileusis, Laser In Situ , Photorefractive Keratectomy , Refractive Surgical Procedures , Astigmatism/surgery , Humans , Hyperopia/surgery , Keratomileusis, Laser In Situ/instrumentation , Lasers, Excimer , Myopia/surgery , Photorefractive Keratectomy/instrumentation , Retrospective Studies , Treatment OutcomeABSTRACT
Corneal transplant become a surgical procedure which is performed with success in more many ophthalmological medico-surgical centers. This way it raised a fully experience concerning selection of cases and the preservation of the graft, surgical technique and the graft rejection. The authors present a general view of the problems concerning keratoplasty making a briefly summary of the indications, contraindications and prognosis of this technique, screening of donor tissue and a little larger one the surgical technique with his problems: trephining, preserving the donor tissue and placing the graft, and also the postoperative problems--suture removal, visual correction and corneal transplant rejection.