ABSTRACT
Cucurbit yellow vine disease (CYVD), caused by the squash bug (Anasa tristis)-transmitted bacterium Serratia marcescens, was first identified in Oklahoma and Texas in 1988 and in Georgia in 2012. S. marcescens is a highly diverse species found in many ecological niches. In previous studies, CYVD strains of S. marcescens formed a closely related group separate from non-CYVD strains based on biological and molecular characterization techniques. Multilocus sequence analysis (MLSA) of six housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using the BOX and ERIC primers were used to assess the genetic diversity of CYVD strains of S. marcescens collected in Georgia together with a strain from Texas and seven non-CYVD strains of S. marcescens. rep-PCR results revealed genetic diversity among CYVD strains while MLSA results showed a 100% similarity across the six loci for all but one of the CYVD strains, which differed at the icd locus by five polymorphisms. For both methods, CYVD strains clustered separately from nonplant-pathogenic S. marcescens strains and were most similar to a rice endophyte strain. One CYVD strain isolated from a squash bug shared genetic similarities with non-CYVD strains, and may be the result of a recombination event between CYVD and non-CYVD strains.
ABSTRACT
Crossbow injuries to the thorax are nowadays uncommon. The type of arrowhead used determines not only the form of entrance wound but often the outcome of these injuries. We report the case of a 38-year-old man who attempted to commit suicide by firing a bolt from a sport crossbow into his heart. Although the bolt penetrated the mediastinum causing a deep intraseptal myocardial lesion and the pre-operative diagnostic procedure delayed the necessary operation, the patient survived.
Subject(s)
Heart Injuries/pathology , Wounds, Penetrating/pathology , Adult , Forensic Medicine , Humans , Male , Suicide, AttemptedABSTRACT
Magnetic resonance and computed tomography scans of L4/5 and L5/S1 levels in 129 patients operated for herniated intervertebral disc were assessed to establish the relationship of facet joint asymmetry, disc prolapse and degenerative disease. Facet angle and spinal stenosis were measured. Statistical analysis revealed significant correlation between facet joint asymmetry and disc disease or degenerative stenosis within lumbar spine.
Subject(s)
Intervertebral Disc Displacement/diagnosis , Lumbar Vertebrae/diagnostic imaging , Lumbar Vertebrae/pathology , Spinal Stenosis/diagnosis , Adolescent , Adult , Aged , Female , Humans , Intervertebral Disc Displacement/complications , Intervertebral Disc Displacement/surgery , Lumbar Vertebrae/surgery , Magnetic Resonance Imaging , Male , Middle Aged , Postoperative Period , Spinal Stenosis/etiology , Tomography, X-Ray ComputedABSTRACT
A retrospective standardized review of 76 cases of cemented hip arthroplasty has been done to evaluate the influence of technical errors on survivorship of prosthesis. Increased bone loss around the prosthesis was correlated to improper geometry of artificial hip joint and deficiency of the cementing technique.
