ABSTRACT
The influence of the high dose of hydrocortisone on the content of galactocerebrosides, sulfocerebrosides and cholesterol was studied in different regions of growing rat brain. The most distinct alterations in content and relationship of glycolipids were demonstrated in brain hemisphere and cerebellum. The results allow to suggest a conclusion about changes in myelin's lipid metabolism and disturbance of myelinogenesis after high dose hydrocortisone injection.
Subject(s)
Brain/drug effects , Cerebrosides/metabolism , Glycolipids/metabolism , Hydrocortisone/pharmacology , Sulfoglycosphingolipids/metabolism , Animals , Brain/metabolism , Cholesterol Esters/metabolism , RatsSubject(s)
Amino Acids/administration & dosage , Brain/metabolism , Cerebrosides/metabolism , Demyelinating Diseases/metabolism , Gangliosides/metabolism , Animals , Brain Chemistry , Cerebrosides/analysis , Chronic Disease , Demyelinating Diseases/chemically induced , Disease Models, Animal , Gangliosides/analysis , Phenylalanine/blood , RatsABSTRACT
The concentration and composition of gangliosides in the cells of L-line and its clones--L-2 and Lebr 1/1, with values of saturation densities being 0.9, 1.6 and 3.2 X 10(5) cells/mc2, respectively, were compared. The transition of populations in the stationary growth phase was shown to be accompanied by an increment of ganglioside concentration and accumulation of di- and three-sialogangliosides. The data obtained are discussed in connection with the previously published results about changing intracellular cAMP content and conA-mediated agglutination at density-dependent growth inhibition L-cell variants.
Subject(s)
Gangliosides/metabolism , L Cells/cytology , Animals , Cell Count , Cell Division , L Cells/metabolism , MiceABSTRACT
The turnover rate of N-acetylneuraminic acid of brain gangliosides (GT1, GD1b, GD1a, GM1) was studied following the injection of 2-14C-acetate. The specific activity of N-acetylneuraminic acid in GD1a and GM1 was found to be higher than in GD1b. The administration of aminasine reduced the specific activity of N-acetylneuraminic acid whereas chlorhydrate increased that of major brain ganglosides.
