ABSTRACT
Most indigenous ethnias from Northern Argentina live in rural areas of "the Gran Chaco" region, where Trypanosoma cruzi is endemic. Serological and parasitological features have been poorly characterized in Aboriginal populations and scarce information exist regarding relevant T. cruzi discrete typing units (DTU) and parasitic loads. This study was focused to characterize T. cruzi infection in Qom, Mocoit, Pit'laxá and Wichi ethnias (N=604) and Creole communities (N=257) inhabiting rural villages from two highly endemic provinces of the Argentinean Gran Chaco. DNA extracted using Hexadecyltrimethyl Ammonium Bromide reagent from peripheral blood samples was used for conventional PCR targeted to parasite kinetoplastid DNA (kDNA) and identification of DTUs using nuclear genomic markers. In kDNA-PCR positive samples from three rural Aboriginal communities of "Monte Impenetrable Chaqueño", minicircle signatures were characterized by Low stringency single primer-PCR and parasitic loads calculated using Real-Time PCR. Seroprevalence was higher in Aboriginal (47.98%) than in Creole (27.23%) rural communities (Chi square, p=4.e(-8)). A low seroprevalence (4.3%) was detected in a Qom settlement at the suburbs of Resistencia city (Fisher Exact test, p=2.e(-21)).The kDNA-PCR positivity was 42.15% in Aboriginal communities and 65.71% in Creole populations (Chi square, p=5.e(-4)). Among Aboriginal communities kDNA-PCR positivity was heterogeneous (Chi square, p=1.e(-4)). Highest kDNA-PCR positivity (79%) was detected in the Qom community of Colonia Aborigen and the lowest PCR positivity in two different surveys at the Wichi community of Misión Nueva Pompeya (33.3% in 2010 and 20.8% in 2014). TcV (or TcII/V/VI) was predominant in both Aboriginal and Creole communities, in agreement with DTU distribution reported for the region. Besides, two subjects were infected with TcVI, one with TcI and four presented mixed infections of TcV plus TcII/VI. Most minicircle signatures clustered according to their original localities, but in a few cases, signatures from one locality clustered with signatures from other village, suggesting circulation of the same strains in the area. Parasitic loads ranged from undetectable to around 50 parasite equivalents/mL, showing higher values than those generally observed in chronic Chagas disease patients living in urban centers of Argentina. Our findings reveal the persistence of high levels of infection in these neglected populations.
Subject(s)
Chagas Disease/epidemiology , DNA, Kinetoplast/genetics , Endemic Diseases , Phylogeny , Trypanosoma cruzi/genetics , Adolescent , Adult , Aged , Argentina/epidemiology , Chagas Disease/ethnology , Chagas Disease/parasitology , Child , Child, Preschool , Humans , Indians, South American , Infant , Middle Aged , Parasite Load , Rural Population , Seroepidemiologic Studies , Trypanosoma cruzi/classification , Trypanosoma cruzi/growth & development , Trypanosoma cruzi/pathogenicity , Vulnerable PopulationsABSTRACT
All patients with Hodgkin's disease should at minimum, undergo staging evaluation. The staging system that has developed in an effort to distinguish patients with different prognose in an anatomic staging system that generally corelates with the tumor burden. In this article the authors describe the early lymph nodes involvement,with a study of patients whose disease has spread to the spleen. Special interest has focused in the study of the early lesions for the complete understanding of the concept of the classical Hodgkin lymphoma
Subject(s)
Humans , Dendritic Cells, Follicular/classification , Hodgkin Disease , Lymphocytes , Lymph Nodes/physiopathologyABSTRACT
El linfoma B rico en células T es una proliferación de células grandes B aisladas en medio de una alta proporción de linfocitos. Biológicamente el linfoma B rico en células T es similar al linfoma de grandes células B convencional, pero se lo considera una entidad debido a los problemas que surgen de su diagnóstico diferencial
Subject(s)
Humans , Azure Stains , Eosine Yellowish-(YS) , Hematoxylin , Lymphoma, Large B-Cell, Diffuse , Lymphoma, B-Cell/classification , Lymphoma, B-Cell/diagnosis , Plasma Cells , Receptors, Complement 3dABSTRACT
All patients with Hodgkins disease should at minimum, undergo staging evaluation. The staging system that has developed in an effort to distinguish patients with different prognose in an anatomic staging system that generally corelates with the tumor burden. In this article the authors describe the early lymph nodes involvement,with a study of patients whose disease has spread to the spleen. Special interest has focused in the study of the early lesions for the complete understanding of the concept of the classical Hodgkin lymphoma
Subject(s)
Humans , Hodgkin Disease/diagnosis , Hodgkin Disease/classification , Hodgkin Disease/pathology , Lymphocytes , Dendritic Cells, Follicular/classification , Lymph Nodes/physiopathologyABSTRACT
El linfoma B rico en células T es una proliferación de células grandes B aisladas en medio de una alta proporción de linfocitos. Biológicamente el linfoma B rico en células T es similar al linfoma de grandes células B convencional, pero se lo considera una entidad debido a los problemas que surgen de su diagnóstico diferencial
Subject(s)
Humans , Lymphoma, B-Cell/classification , Lymphoma, B-Cell/diagnosis , Plasma Cells , Lymphoma, Large B-Cell, Diffuse/diagnosis , Hematoxylin/diagnosis , Eosine Yellowish-(YS)/diagnosis , Azure Stains/diagnosis , Receptors, Complement 3dABSTRACT
De las lesiones conocidad como premalignas la neoplasia intraepitelial prostática (PIN), es la que adquiere mayor relevancia, debido a su estrecha asociación con el cáncer invasor de proóstata. Su incidencia en hombres menores de 60 años ha sido probada y justifica las punciones repetidas en aquellos pacientes que la presenten. En este trabajo se realiza un análisis de 937 biopsias de próstata, y se correlaciona el hallazgo de PIN en sus diferentes grados con el comportamiento del antígeno prostático específico (PSA), la edad del paciente y la existencia de cáncer de próstata(AU)
Subject(s)
Humans , Male , Prostatic Intraepithelial Neoplasia/diagnosis , Prostatic Intraepithelial Neoplasia/pathology , Biopsy , Prostatic Neoplasms/diagnosis , Prostatic Neoplasms/classification , Prostate-Specific AntigenABSTRACT
De las lesiones conocidad como premalignas la neoplasia intraepitelial prostática (PIN), es la que adquiere mayor relevancia, debido a su estrecha asociación con el cáncer invasor de proóstata. Su incidencia en hombres menores de 60 años ha sido probada y justifica las punciones repetidas en aquellos pacientes que la presenten. En este trabajo se realiza un análisis de 937 biopsias de próstata, y se correlaciona el hallazgo de PIN en sus diferentes grados con el comportamiento del antígeno prostático específico (PSA), la edad del paciente y la existencia de cáncer de próstata
Subject(s)
Humans , Male , Biopsy , Prostatic Intraepithelial Neoplasia/diagnosis , Prostatic Intraepithelial Neoplasia/pathology , Prostatic Neoplasms/classification , Prostatic Neoplasms/diagnosis , Prostate-Specific AntigenABSTRACT
La técnica de coloración argirófila coloidal para demostrar las proteínas a las regiones de organización nucleolar han probado ser de simple y fácil aplicación en cortes de parafina.Se ha aplicado en el estudio de diez casos de linfomas de grandes células anaplásicas primarios y secundarios a enfermedad de Hodgkin ,mostrando positividad para antígeno Ki1 (CD 30) y EMA.Se halló correlación entre el número de regiones de organización nucleolar y el grado proliferativo, ploidia y grado histológico de malignidad.Los valores hallados son similares a los hallados en linfomas malignos no Hodgkin de alto grado y en los diferentes tipos de células de Sternberg o de Hodgkin de la enfermedad de Hodgkin,además se observaron frecuentes y grandes depósitos argirófilos semejantes a los hallados en la enfermedad de Hodgkin.Se sugiere la posible relación entre los linfomas de grandes células anaplásicas y la enfermedad de Hodgkin
Subject(s)
Humans , Lymphoma, Large B-Cell, Diffuse/pathologyABSTRACT
La técnica de coloración argirófila coloidal para demostrar las proteínas a las regiones de organización nucleolar han probado ser de simple y fácil aplicación en cortes de parafina.Se ha aplicado en el estudio de diez casos de linfomas de grandes células anaplásicas primarios y secundarios a enfermedad de Hodgkin ,mostrando positividad para antígeno Ki1 (CD 30) y EMA.Se halló correlación entre el número de regiones de organización nucleolar y el grado proliferativo, ploidia y grado histológico de malignidad.Los valores hallados son similares a los hallados en linfomas malignos no Hodgkin de alto grado y en los diferentes tipos de células de Sternberg o de Hodgkin de la enfermedad de Hodgkin,además se observaron frecuentes y grandes depósitos argirófilos semejantes a los hallados en la enfermedad de Hodgkin.Se sugiere la posible relación entre los linfomas de grandes células anaplásicas y la enfermedad de Hodgkin
Subject(s)
Humans , Lymphoma, Large B-Cell, Diffuse/pathologyABSTRACT
Occurrence of vomiting and diarrhea in patients with acquired immunodeficiency syndrome was most frequently attributed to enteropathogens organism that invade the gastrointestinal tract because of the immunologic unbalance of the host... Among several causes the cryptosporidium has been detected quite often and its predominant localization was the gastrointestinal tract, although other extraintestinal sites has also been reported. In both cases, erosive congestive gastritis was found, while histological examination showed cryptosporidium in gastric biopsy specimens.
Subject(s)
AIDS-Related Opportunistic Infections/parasitology , Cryptosporidiosis/complications , Gastritis/parasitology , AIDS-Related Opportunistic Infections/pathology , Adult , Biopsy , Cryptosporidiosis/pathology , Fatal Outcome , Female , Gastritis/pathology , HumansABSTRACT
La aparición de vómitos y diarrea en pacientes con síndrome de inmunodeficiencia adquirida ha sido atribuido frecuentemente a organismos enteropatógenos que invaden el tracto gastrointestinal como consecuencia del trastorno inmunológico del huésped. El cryptosporidium ha sido detectado con alguna prevalencia y su localización predominante es el tracto gastrointestinal, a pesar que otros sitios han sido notificados. Se presentan dos casos con diagnóstico endoscópico de gastritis congestiva-erosiva con presencia de criptosporidium en las biopsias gástricas. Ambos pacientes cursaban un estadío terminal de su enfermedad (AU)
Subject(s)
Humans , Female , Adult , Gastritis/etiology , Acquired Immunodeficiency Syndrome/complications , Cryptosporidiosis/complications , Gastritis/pathology , Biopsy , Fatal OutcomeABSTRACT
La aparición de vómitos y diarrea en pacientes con síndrome de inmunodeficiencia adquirida ha sido atribuido frecuentemente a organismos enteropatógenos que invaden el tracto gastrointestinal como consecuencia del trastorno inmunológico del huésped. El cryptosporidium ha sido detectado con alguna prevalencia y su localización predominante es el tracto gastrointestinal, a pesar que otros sitios han sido notificados. Se presentan dos casos con diagnóstico endoscópico de gastritis congestiva-erosiva con presencia de criptosporidium en las biopsias gástricas. Ambos pacientes cursaban un estadío terminal de su enfermedad
Subject(s)
Humans , Female , Adult , Cryptosporidiosis/complications , Gastritis/etiology , Acquired Immunodeficiency Syndrome/complications , Biopsy , Fatal Outcome , Gastritis/pathologyABSTRACT
Occurrence of vomiting and diarrhea in patients with acquired immunodeficiency syndrome was most frequently attributed to enteropathogens organism that invade the gastrointestinal tract because of the immunologic unbalance of the host... Among several causes the cryptosporidium has been detected quite often and its predominant localization was the gastrointestinal tract, although other extraintestinal sites has also been reported. In both cases, erosive congestive gastritis was found, while histological examination showed cryptosporidium in gastric biopsy specimens.
ABSTRACT
We report on the histologic changes occurring in single cutaneous lesions, from six active lepromatous patients, 1 week following the administration of three daily intradermal injections, 35 micrograms each, of recombinant interferon gamma (rIFN-gamma). Except for a strong induration at the injection site, rIFN-gamma produced no adverse systemic reactions and was able to promote a remarkable influx of T-lymphocytes, mononuclear phagocytes with large nuclei, nonvacuolated cytoplasm, and reduced lysozyme reactivity. Furthermore, despite no clear-cut reduction of mycobacterial dermal burden, bacilli showed a clear increase in the granular appearance. Present findings provide a basis for further elucidation of rIFN-gamma as an additional tool for leprosy treatment.
