ABSTRACT
Clostridium difficile-isolates associated with CDI in different healthcare facilities in Hesse were analysed. The most common ribotypes were 001 (31.1%) and 027 (27.0%). The proportion of ribotype 027 among regional C. difficile-isolates was 10.8% in North Hesse, 17.2% in Middle Hesse, and 33.5% in the Rhine-Main Metropolitan Area. In the latter region, ribotype 027 was the most prevalent ribotype.
Subject(s)
Clostridioides difficile/classification , Cross Infection/epidemiology , Enterocolitis, Pseudomembranous/epidemiology , Ribotyping , Clostridioides difficile/genetics , Clostridioides difficile/isolation & purification , Cross Infection/microbiology , Enterocolitis, Pseudomembranous/microbiology , Feces/microbiology , Germany/epidemiology , Humans , PrevalenceABSTRACT
In 2011, the Shiga toxin- and extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli O104:H4 caused a serious outbreak of gastroenteritis in Germany. This strain carried bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid. During screening of individuals at risk for acquisition of the epidemic E. coli O104:H4, we isolated another ESBL-producing and Shiga toxin-positive E. coli belonging to serotype O91:H14 from feces of a human patient. Interestingly, the patient also carried a further ESBL-producing but Shiga toxin-negative E. coli. Both strains harbored bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid, which was indistinguishable regarding size and plasmid restriction pattern from the plasmid of the epidemic E. coli O104:H4 strain. The patient had traveled to India 6 months prior to the isolation of the E. coli strains. This is the first report of an ESBL-producing, Shiga toxin-positive E. coli of serogroup O91. Our data suggest a high propensity of the IncI1-ST31 plasmid to spread in the human and/or animal population.
Subject(s)
Escherichia coli Infections/microbiology , Escherichia coli Proteins/metabolism , Feces/microbiology , Plasmids/analysis , Shiga-Toxigenic Escherichia coli/enzymology , Shiga-Toxigenic Escherichia coli/isolation & purification , beta-Lactamases/metabolism , DNA, Bacterial/genetics , Escherichia coli Proteins/genetics , Gene Transfer, Horizontal , Germany , Humans , India , Molecular Weight , Restriction Mapping , Serotyping , Shiga-Toxigenic Escherichia coli/classification , Shiga-Toxigenic Escherichia coli/genetics , Travel , beta-Lactamases/geneticsSubject(s)
Tuberculosis/diagnosis , Tuberculosis/prevention & control , Tuberculosis/transmission , Adolescent , Adult , Communicable Disease Control , Contact Tracing , Disease Progression , Female , Germany , Humans , Incidence , Interferon-gamma/metabolism , Male , Predictive Value of Tests , Prognosis , Prospective Studies , Risk Assessment , Treatment Outcome , Young AdultABSTRACT
Clostridium difficile is the most common cause of antibiotic-associated diarrhoea in hospitals and other healthcare facilities. The elderly are particularly susceptible and at increased risk for adverse outcome as a result of C. difficile infection. The aim of this study was to determine the prevalence of C. difficile colonization among residents of nursing homes in Hesse and to compare it with the prevalence in the general population living outside long-term care facilities (LTCF). We assessed possible risk factors for C. difficile colonization and determined the genotype of circulating strains. C. difficile was isolated from 11/240 (4.6%) nursing home residents and 2/249 (0.8%) individuals living outside LTCF (pâ=â0.02). Ten of 11 (90.9%) isolates from nursing homes and one of two isolates from the population outside LTCF were toxigenic. The prevalence of C. difficile colonization varied from 0% to 10% between different nursing homes. Facilities with known actual or recent CDI cases were more likely to have colonized residents than facilities without known CDI cases. C. difficile PCR-ribotypes 014 and 001 were the most prevalent genotypes and accounted for 30% and 20% of toxigenic isolates in nursing homes, respectively. Interestingly, no individuals carried the epidemic strain PCR-ribotype 027. Our results suggest that residents of nursing homes in Germany are at high risk for colonization by virulent C. difficile strains. The high prevalence of C. difficile colonization in nursing homes underscores the importance of good adherence to standard infection control precautions even in the absence of a diagnosed infection. They also emphasize the need for specific programs to increase the awareness of healthcare professionals in LTCF for CDI.
