1.
Cold Spring Harb Symp Quant Biol
; 60: 337-48, 1995.
Article
in English
| MEDLINE
| ID: mdl-8824407
Subject(s)
Membrane Fusion/physiology , Nerve Tissue Proteins/metabolism , Saccharomyces cerevisiae Proteins , Synaptic Vesicles/metabolism , Vesicular Transport Proteins , Amino Acid Sequence , Animals , Carrier Proteins/genetics , Carrier Proteins/metabolism , Decapodiformes , GTP-Binding Proteins/genetics , GTP-Binding Proteins/metabolism , Membrane Proteins/genetics , Membrane Proteins/metabolism , Molecular Sequence Data , Nerve Tissue Proteins/genetics , Peptides/pharmacology , Sequence Homology, Amino Acid , Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins , Synaptic Vesicles/drug effects , rab3 GTP-Binding Proteins
2.
Neuron
; 8(2): 387-97, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1310865
ABSTRACT
Binding of cAMP by the five neuronal isoforms (N1-5) of the regulatory (R) subunit of the Aplysia cAMP-dependent protein kinase is diminished in sensory neurons stimulated to produce long-term presynaptic facilitation. To determine how the cAMP-binding activity of the R subunits is lost, we isolated cDNAs encoding N4, which is a homolog of mammalian RI. Immunoblots with antisera raised against the R protein overexpressed in E. coli show that the diminished binding activity, which occurs in long-term facilitation, results from coordinate loss of R protein isoforms. No change was detected in the amount of transcripts for R subunits, suggesting that the down-regulation results from enhanced proteolytic turnover.