Hypocholesterolemic potential of Bacillus amyloliquefaciens KAVK1 modulates lipid accumulation on 3T3-L1 adipose cells and high fat diet-induced obese rat model.

The significance of microorganisms occurring in foods is predominantly targeted due to their application for identifying a novel range of the bacterial spectrum. Diverse microbial species are capable of exhibiting potential pharmacological activities like antimicrobial and anticancer. Microbial strains capable of reducing obesity-related syndromes have also been reported. In the present study, the hypocholesterolemic efficacy of Bacillus amyloliquefaciens isolated from dairy products was scrutinised by in vitro (3T3-L1 adipose cells) and in vivo (high-fat diet-induced obese Wistar albino rats) methods. Potential cholesterol-lowering isolates were screened using a plate assay method and optimised by physical parameters. Molecular identification of the topmost five cholesterol-lowering isolates was acquired by amplification of the 16 S rRNA gene region. Bacillus amyloliquefaciens strain KAVK1, followed by strains KAVK2, KAVK3, KAVK4, and KAVK5 were molecularly determined. Further, cholesterol-lowering strains degraded the spectral patterns determined by the side chain of a cholesterol molecule. The anti-lipase activity was demonstrated using the porcine pancreatic lipase inhibitory method and compared with the reference compound Atorvastatin. Lyophilised strain KAVK1 revealed maximum pancreatic lipase inhibition. Strain KAVK1 attenuated lipid accumulation in 3T3-L1 adipose cell line predicted by Oil Red O staining method. Significant reduction of body weight and change in lipid profile was recognised after the supplement of KAVK1 to obese rats. Histopathological changes in organs were predominantly marked. The result of this study implies that the cholesterol-lowering B. amyloliquefaciens KAVK1 strain was used to treat hypercholesterolemia.

Molecular intervention of colon cancer and inflammation manifestation by tannin capped biocompatible controlled sized gold nanoparticles from Terminalia bellirica: A green strategy for pharmacological drug formulation based on nanotechnology principles.

Among the diverse nanomaterials, gold nanoparticles (AuNps) are utilised for various therapeutic application due to the distinct physical, chemical properties and biocompatibility. Synthesis of gold nanoparticles using plants is the promising route. This method is low cost, eco-friendly and higher biological activities. In this present study, Gold nanoparticles were synthesised from fruit extract of Terminalia bellirica fruit extract. Their anticancer and anti-inflammatory activity was evaluated against colorectal cancer cell line (HT29) and TNBS-induced zebrafish model. Highly stable tannin capped gold nanoparticles were synthesised from fruit extract broth of Terminalia bellirica rapidly. Structural and functional properties of the synthesised nanoparticles were studied by Fourier transform infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM) equipped with energy-dispersive atomic X-ray spectroscopy (EDAX) and X-ray diffraction (XRD). All the characterisation studies reveal highly stable, crystalline, phytochemicals, mainly tannin doped, spherical, 28 nm controlled sized gold nanoparticles. The molecular mechanism of anticancer activity was studied by determining cancer markers' expression, which was studied using quantitative real-time polymerase chain reaction (qPCR). Antioxidative enzymes' status and apoptosis changes were also investigated. Synthesised nanoparticles brought a drastic reduction of all the tested cancer markers' expression. Notable changes in antioxidative enzymes' status and a good sign of apoptosis were observed in nanoparticles' treatment. The anti-inflammatory activity was studied against TNBS-induced zebrafish model, which was confirmed by determining inflammatory markers' expression TNF-α, iNOS (induced Nitric Oxide Synthase) and histopathological examination. Nanoparticles' treatment recorded a drastic reduction of inflammatory markers' expression. No marked sign of inflammation was also observed in histopathological analysis of the nanoparticles' treatment group. The present study suggests the possible utilisation of T. bellirica-mediated gold nanoparticles as an effective therapeutic agent against a prolonged inflammatory disease that progressively develops into cancer.

Terminalia chebula and Ficus racemosa principles mediated repression of novel drug target Las R - the transcriptional regulator and its controlled virulence factors produced by multiple drug resistant Pseudomonas aeruginosa - Biocompatible formulation against drug resistant bacteria.

Pseudomonas aeruginosa- major group of an aerobic bacteria associated with nosocomial and other life threatening infections. Diverse virulence factors produced by P. aeruginosa is due to distinct molecular cell signaling mechanism termed as quorum sensing (QS). Interfering with normal QS mechanism by active biomolecules is an effective strategy for attenuating its virulence. With this objective, the present study is undertaken to evaluate the inhibition of quorum sensing of clinical isolate of P. aeruginosa by repression of Las R-a transcriptional regulator for QS by ethanol extract of Terminalia chebula and Ficus racemosa. Las R repression by the plant extracts was measured in inhibition of various virulence factors like biofilm, pyocyanin production, total proteolytic activity, swarming and twisting motility. Fabrication of the extracted metabolites on the wound dressing and its effect on anti bacterial activity was also investigated. Compatibility of plant extracts on zebra fish development and blood cells was further studied. P. aeruginosa was isolated from the post operative patient and the isolated pure culture was identified by cultural, biochemical, molecular characteristics. Active principles of both the plants were readily extracted in ethanol and effectively repressed the expression of Las R. Both the tested plant extracts effectively repressed Las R expression which in turn affect the production of various virulence factors like biofilm formation, pyocyanin production, swarming motility, twisting motility, total proteolytic activity, cell adhesion and signaling molecule acyl honoserine lactone (AHL) production. Plant extract treatment brought about drastic reduction of all the tested virulence factors and AHL production. Extracted metabolites were fabricated on the wound dressing material adopting simple dip or immersion method reveals uniform coating, effective embedding of phytochemicals with the fibers and retained the anti bacterial activity against P. aeruginosa. Biocompatibility studies with zebra fish model shows both the tested plant extracts treatment was not exhibited any sign of toxicity on the developmental stages of Zebra fish. Hemolysis and changes in anti oxidative enzymes were not recorded in the plant extracts treated blood which demonstrated the best biocompatibility of the tested plant extracts. These results shows that the presence of potential phytochemicals in the ethanolic extract of Terminalia chebula and Ficus racemosa effectively represses the Las R followed by inhibition of quorum sensing mediated virulence factors production may be useful in the lead of anti bacterial drugs.

A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.

The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility. In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan­iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique. Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm. Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm. FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles. Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies. Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains. 100 µg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively. Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains. Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis. Composite prompted 50% of cytotoxicity at 80 µg/mL unmistakably uncovers cytotoxicity against A549 cells. Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 µg. Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death. The present findings would propose the conceivable usage of chitosan­iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.