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1.
Article in English | MEDLINE | ID: mdl-36767685

ABSTRACT

There is world-wide generation of food waste daily in significant amounts, leading to depletion of natural resources and deteriorating air quality. One-third of global food produced is wasted laterally with the food value chain. Carbon footprint is an efficient way of communicating the issues related to climate change and the necessity of changing behavior. Valorization or utilization of food wastes helps in resolving issues related to environment pollution. Reduction in the carbon footprint throughout the chain of food supply makes the whole process eco-friendly. Prevailing food waste disposal systems focus on their economic and environmental viability and are putting efforts into using food waste as a resource input to agriculture. Effective and advanced waste management systems are adopted to deal with massive waste production so as to fill the gap between the production and management of waste disposal. Food waste biorefineries are a sustainable, eco-friendly, and cost-effective approach for the production of platform chemicals, biofuels, and other bio-based materials. These materials not only provide sustainable resources for producing various chemicals and materials but have the potential to reduce this huge environmental burden significantly. In this regard, technological advancement has occurred in past few years that has proven suitable for tackling this problem.


Subject(s)
Refuse Disposal , Waste Management , Carbon Footprint , Food , Biofuels
2.
Environ Sci Pollut Res Int ; 30(4): 8526-8539, 2023 Jan.
Article in English | MEDLINE | ID: mdl-35554831

ABSTRACT

The major global concern on energy is focused on conventional fossil resources. The burning of fossil fuels is an origin of greenhouse gas emissions resulting in the utmost threat to the environment and subsequently which leads to global climate changes. As far as sustainability is concerned, fuels and materials derived from organic or plant wastes overcome this downside establishing the solution to the fossil resource crisis. In this context, exploration of agricultural residue appears to be a suitable alternative of non-renewable resources to support the environmental feasibility and meet the high energy crisis. The use of agricultural waste as a feedstock for the biorefinery approach emerges to be an eco-friendly process for the production of biofuel and value-added chemicals, intensifying energy security. Therefore, a prospective choice of this renewable biomass for the synthesis of green fuel and other green biochemicals comes up with a favorable outcome in terms of cost-effectiveness and sustainability. Exploiting different agricultural biomass and exploring various biomass conversion techniques, biorefinery generates bioenergy in a strategic way which eventually fits in a circular bioeconomy. Sources and production of agricultural waste are critically explained in this paper, which provides a path for further value addition by various technologies. Biorefinery solutions, along with a life cycle assessment of agricultural waste biomass toward a wide array of value-added products aiding the bioeconomy, are summarized in this paper.


Subject(s)
Fossil Fuels , Plants , Biofuels , Biomass , Prospective Studies , Recycling
3.
Arch Microbiol ; 204(1): 23, 2021 Dec 17.
Article in English | MEDLINE | ID: mdl-34918191

ABSTRACT

The production of useful phenolic flavor compounds by utilizing Lactobacillus acidophilus MTCC 10307 was studied. Ferulic acid, vanillic acid and vanillin were obtained as the significant phenolic acids from the fermentation medium. The compounds were identified and quantified by high-performance thin-layer chromatography. The phenolic acids were detected for 15 days. A maximum quantity of ferulic acid was quantified on the 9th day of incubation and the quantity decreased on further incubation. While the utmost amounts of vanillic acid and vanillin were detected on the 12th day of incubation. The concentration of carbohydrates from the de-starched bagasse was also estimated and was contrasted with that of the original (control) bagasse. The growth pattern of the microorganism was also studied. The quantity of ferulic acid measured per kg of sugarcane bagasse on the 9th day of incubation was determined to be approximately 275 mg whereas 18 mg and 15 mg of vanillic acid and vanillin, respectively, were measured per kg of bagasse on the 12th day of incubation. Ferulic acid esterase was isolated and the fermentation conditions such as pH, temperature and incubation period were standardized for the maximum recovery of the enzyme. The results revealed that in optimized condition, ferulic acid esterase yield was found to be 2.2 U ml-1 at 35 °C, whereas ferulic acid esterase yield was 2.3 U ml-1 at 6.5 pH and 2.4 U ml-1 after 60 h of the incubation period.


Subject(s)
Lactobacillus acidophilus , Saccharum , Cellulose
4.
Prep Biochem Biotechnol ; 49(8): 744-758, 2019.
Article in English | MEDLINE | ID: mdl-31050587

ABSTRACT

Lignocellulosic biomass (LB) is the renewable feedstock for the production of fuel/energy, feed/food, chemicals, and materials. LB could also be the versatile source of the functional oligosaccharides, which are non-digestible food ingredients having numerous applications in food, cosmetics, pharmaceutical industries, and others. The burgeoning functional food demand is expected to be more than US$440 billion in 2022. Because of higher stability at low pH and high temperature, oligosaccharides stimulate the growth of prebiotic bifidobacteria and lactic acid bacteria. Xylooligosaccharides (XOS) are major constituents of oligosaccharides consisting of 2-7 xylose monomeric units linked via ß-(1,4)-linkages. XOS can be obtained from various agro-residues by thermochemical pretreatment, enzymatic or chemoenzymatic methods. While thermochemical methods are fast, reproducible, enzymatic methods are substrate specific, costly, and produce minimum side products. Enzymatic methods are preferred for the production of food grade and pharmaceutically important oligosaccharides. XOS are potent prebiotics having antioxidant properties and enhance the bio-adsorption of calcium and improving bowel functions, etc. LB can cater to the increasing demand of oligosaccharides because of their foreseeable amount and the advancements in technology to recover oligosaccharides. This paper summarizes the methods for oligosaccharides production from LB, classification, and benefits of oligosaccharides on human health.


Subject(s)
Biotechnology/methods , Glucuronates/metabolism , Lignin/metabolism , Oligosaccharides/metabolism , Biomass , Dietary Fiber/analysis , Humans , Plants/enzymology , Plants/metabolism , Xylosidases/metabolism
5.
Indian J Exp Biol ; 53(12): 819-27, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26742327

ABSTRACT

Bioethanol, is a potential alternate source of energy, renewable and safe. Ethanol production from value added food and feedstock has also not shown growth as estimated. Of late, the second generation processes of production of ethanol, such as from lignocellulosic biomass out of agricultural/domestic waste has been gaining considerable momentum. Here, we explored a new approach for optimizing the conditions of physiochemical pretreatment as well as fermentation process using peels of Ananas cosmosus as substrate and immobilized yeast Pachysolen tannophilus MTCC.1077 and Pichia stipitis NCIM 3498. We have also studied the influence of process variables such as incubation temperature, inoculum concentration and different nutrients on ethanol production. Pulverized peels of A. cosmosus recorded 25 ± 0.31% cellulose, 28 ± 0.18% hemicellulose and 8 ± 0.07% of lignin on dry solid (DS) basis. Peels of A. cosmosus delignified with 1% H2SO4 yielded 18.89% glucose, 38.81% xylose and 29.31% fructose under thermochemical pretreatment using autoclave (121 degrees C, 20 min.), with a hydrolytic efficiency of 75.52 ± 0.45%. FTIR spectroscopy results not only indicated the penetration of H2SO4 in the amorphous region of the biomass and degradation of hemicelluloses but also showed the structural differences before and after pretreatment. The enzymes required for hydrolysis were prepared from culture supernatants of Trichoderma reesei NCIM 1052 using wheat bran as carbon source under submerged fermentation conditions on rotatory shaker incubator (at 28 degrees C for 10 days). Enzyme activity (U/ml) of crude cellulase produced by T. reesei NCIM 1052 was 311.1 µmole/ml/min. Delignified A. cosmosus peel yielded 51.71 ± 0.44 g/l glucose when enzymatically hydrolysed by crude cellulase at the substrate enzyme ratio of 1:5. Simultaneous saccharification and fermentation (SSF) of peels of A. cosmosus by crude cellulase and separately entrapped Pichia stipitis NCIM 3498 (now known as Scheffersomyces stipitis) and Pachysolen tannophilus MTCC 1077 cells in calcium alginate beads were also investigated in the present study. The fermentation experiments were carried out at flask level. The processing parameters setup for reaching a maximum response for ethanol production was obtained when applying the optimum values for temperature (32 degrees C), inoculum level (6%) and fermentation medium (ammonium sulphate, KH2PO4, peptone and yeast extract) for P. tannophilus MTCC 1077 and temperature (30 degrees C), inoculum level (2%) and fermentation medium (ammonium sulphate, KH2PO4, peptone and yeast extract) for S. stipitis NCIM 3498. Maximum ethanol concentration 10.5 g/l and 10.9 g/l was obtained from P. tannophilus MTCC 1077 and S. stipitis NCIM 3498, respectively at the optimized process conditions in anaerobic batch fermentation.


Subject(s)
Ananas/microbiology , Biofuels , Ethanol/metabolism , Fruit/microbiology , Pichia/metabolism , Saccharomycetales/metabolism , Ananas/metabolism , Biocatalysis , Cellulose/metabolism , Fermentation , Fruit/metabolism , Hydrolysis , Industrial Microbiology , Lignin/metabolism , Polysaccharides/metabolism , Temperature , Time Factors
6.
Arch Microbiol ; 196(4): 235-48, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24535490

ABSTRACT

The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predation. If all epibiotic predators are antibiotic producers, it is worth testing whether all antibiotic producers are predators too. We show here that Streptomyces are non-obligate epibiotic predators of other microorganisms and that predatory abilities are widespread in this genus. We developed a test for predatory activity which revealed that a large proportion of traditionally isolated Streptomyces strains and all oligophilic Streptomyces isolates show predatory activity. Those that did not show predatory ability on first challenge could do so after many generations of selection or acclimation. Using time-lapse photomicrography, we demonstrate that the growth of the tips of Streptomyces hyphae is accompanied by disappearance of cells of other bacteria in the vicinity presumably due to lysis. Predatory activity is restricted to surface growth and is not obligately associated with antibiotic production in conventional culture. However, some of the genes crucial to the regulation of secondary metabolite pathways are differentially expressed during predatory growth on different prey species as compared to saprophytic growth. Our findings strengthen the association between epibiotic predation and antibiotic production.


Subject(s)
Food Chain , Gene Expression Regulation, Bacterial , Streptomyces/physiology , Anti-Bacterial Agents/biosynthesis , Bacteria/genetics , Bacteria/metabolism , India , Soil Microbiology , Streptomyces/genetics , Streptomyces/growth & development , Streptomyces/isolation & purification , Streptomyces/metabolism
7.
AMB Express ; 2(1): 65, 2012 Dec 07.
Article in English | MEDLINE | ID: mdl-23217124

ABSTRACT

Agro-industrial wastes are generated during the industrial processing of agricultural products. These wastes are generated in large amounts throughout the year, and are the most abundant renewable resources on earth. Due to the large availability and composition rich in compounds that could be used in other processes, there is a great interest on the reuse of these wastes, both from economical and environmental view points. The economic aspect is based on the fact that such wastes may be used as low-cost raw materials for the production of other value-added compounds, with the expectancy of reducing the production costs. The environmental concern is because most of the agro-industrial wastes contain phenolic compounds and/or other compounds of toxic potential; which may cause deterioration of the environment when the waste is discharged to the nature. Although the production of bioethanol offers many benefits, more research is needed in the aspects like feedstock preparation, fermentation technology modification, etc., to make bioethanol more economically viable.

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