Effect Of Lead On The Skin And Health Of Female Dermatitis Patients Through Cosmetics.

BACKGROUND: Cosmetics have been a part of routine body care not only for the upper classes but also for the middle and lower classes since the dawn of civilization. Cosmetic formulations are in more demand as the public's interest in skin whitening grows. The contamination of cosmetics with heavy metals is a major concern as they containing heavy metals and pose a major risk to human health. This study looks in to the effects of Lead on human skin. METHODS: In this cross sectional study different products were examined. The matrices (scalp hair, blood, serum and nails) of reference and dermatitis cosmetic female patients (seborrhoeic dermatitis, rosacea, allergic contact dermatitis, and irritant contact dermatitis) and cosmetic samples were used in a 2:1 mixture of HNO3 (65%) and H2O2 (30%), and oxidation was performed using a microwave. The oxidized beauty and biological specimen underwent electrothermal atomic emission spectrophotometry after microwave-assisted acid digestion. The validity and precision of the methodology were verified using certified reference materials. Cosmetic products (lipstick, face powder, Eye Liner and Eye shadow) of different brands contain Pb concentrations in the ranges of 50.5-120 µg/g, 14.6-30.7 µg/g, 2.87-4.25 µg/g and 15.3-21.6 µg/g, respectively. RESULTS: In the present study, cosmetic products (lipstick (N=15), face powder (N=13), eye liner (N=11), eye shadow (N=15) and female patients with dermatitis (N=252) residing in Hyderabad city, Sindh, Pakistan, was investigated. The outcome of this investigation showed significantly higher levels of Pb in biological samples (blood and scalp hair) of different types of female dermatitis patients than in reference subjects (p<0.001). CONCLUSIONS: The cosmetic products, especially with regard to heavy metals adulteration, are in use by the female population.

A rapid ultrasonic energy assisted preconcentration method for simultaneous extraction of lead and cadmium in various cosmetic brands using deep eutectic solvent: A multivariate study.

An innovative ultrasonic assisted microextraction method using a deep eutectic solvent for entrapping the organ metallic complexes was developed to pre-concentrate the trace quantities of cadmium (Cd) and lead (Pb) in lipsticks and eye shadows of different brands prior to flame atomic absorption spectrometry analysis. A deep eutectic solvent (DES) was used in this study, prepared by mixing metal salt (ZnCl2) and hydrogen bond donors such as (acetamide) in different ratio. The different branded cosmetic items, lipstick and eye shadows, obtained from local market and subjected to acid digestion prior to application of proposed ultrasonic assisted microextraction method based on deep eutectic solvent (UAµE-DES) with the aid of mechanical and ultrasound energies. Ammonium pyrrolidine dithiocarbamate was used as a ligand to made hydrophobic complexes of Cd and Pb, entrapped in DES in the presence of aprotic solvent. The seven variables, mixing ratio of ZnCl2 and Acetamide, DES volume, Ultrasound and Conventional shaking time, pH, tetrahydrofuran and complexing reagent volume were optimized by multivariate approach for the optimum recovery of Cd and Pb from real cosmetic items. The resulted data indicates that ultrasound energy assisted extraction time, volume of DES and pH were most significant variables. The precision and validity of developed procedure were checked by the spiked known certified standards in real samples. The resulted data indicated that the lipsticks have different glittering colors contains high contents of Pb and Cd, whereas dark colors of eye shadows contain high level of both elements.