ABSTRACT
BACKGROUND: Matrix Metalloproteinases (MMPs) are directly responsible for pathogenesis of periodontal diseases and their activity is regulated by Tissue Inhibitor of Metalloproteinases (TIMPs). This study was aimed to evaluate changes in gingival crevicular fluid (GCF) levels of MMP-1 and TIMP-1 in periodontal health and disease. MATERIALS AND METHOD: Clinical parameters were recorded and GCF samples were collected from 30 subjects with chronic generalised periodontitis and 20 periodontally healthy subjects. Subjects with periodontitis underwent scaling and root planing (SRP). GCF samples were collected and clinical parameters were recorded again after 1 month of SRP. GCF levels of MMP-1 and TIMP-1 were detected by ELISA. RESULTS: GCF levels of MMP-1 were significantly increased in subjects with periodontitis at baseline (P0) as compared to periodontally healthy subjects (C). GCF levels of MMP-1 reduced significantly in subjects with periodontitis after treatment (P1) as compared to P0. GCF levels of TIMP-1 were significantly reduced in P0 as compared to C. GCF levels of TIMP-1 increased significantly in P1 as compared to P0. CONCLUSION: Substantial elevation in GCF levels of MMP-1 and reduction in TIMP-1 were found in periodontitis as compared to healthy subjects. GCF levels of MMP-1 and TIMP-1 improved significantly after treatment.
ABSTRACT
AIM: Hyaluronic acid is a glycosaminoglycan with anti-inflammatory & anti edematous properties. This study is seeking to ascertain whether Hyaluronic acid gel formulation is clinically and microscopically effective in treatment of gingivitis. METHODS: In longitudinal, randomized, and placebo-controlled clinical trial, 105 patients with chronic plaque induce gingivitis were randomly divided into three groups; negative control group, placebo control group and test group. Patients were instructed to apply gel on inflamed gingiva twice daily in addition with routine oral hygiene maintenance. The clinical parameters Plaque Index (PI), Gingival Index(GI) and Papilla Bleeding Index (PBI) were determined at intervals of 1 week, 2 weeks and 4 weeks from baseline, microbiological parameters were monitored at the interval of 4 weeks from baseline. RESULTS: An improvement of all clinical variables was observed (p<0.05) for all treatment modalities. Clinically, There is significant difference (p<0.05) for GI & PBI in test group as compared to other groups, but reduction in PI was non-significant. In negative control and placebo control groups, the difference between clinical parameters was non-significant. Statistically significant (p<0.05) reduction in percentage of anaerobic gram negative bacilli and relative increase of gram positive coccoid cells was seen in all treatment groups at 4 weeks as compared to baseline. However, results were not statistically significant (p>0.05) in pair wise comparison in between groups. CONCLUSION: Local application of 0.2 % HA gel adjunct to non surgical periodontal treatment provided a significant improvement in clinical parameters than placebo control and negative control groups. Microbiologically experimental group does not showed any spastically significant results.
ABSTRACT
OBJECTIVES: The present study aims to clinically compare and evaluate subepithelial connective tissue graft and the GTR based root coverage in treatment of Miller's Class I gingival recession. STUDY DESIGN: 30 patients with at least one pair of Miller's Class I gingival recession were treated either with Subepithelial connective tissue graft (Group A) or Guided tissue regeneration (Group B). Clinical parameters monitored included recession RD, width of keratinized gingiva (KG), probing depth (PD), clinical attachment level (CAL), attached gingiva (AG), residual probing depth (RPD) and % of Root coverage(%RC). Measurements were taken at baseline, three months and six months. A standard surgical procedure was used for both Group A and Group B. Data were recorded and statistical analysis was done for both intergroup and intragroup. RESULTS: At end of six months % RC obtained were 84.47% (Group A) and 81.67% (Group B). Both treatments resulted in statistically significant improvement in clinical parameters. When compared, no statistically significant difference was found between both groups except in RPD, where it was significantly greater in Group A. CONCLUSIONS: GTR technique has advantages over subepithelial connective tissue graft for shallow Miller's Class I defects and this procedure can be used to avoid patient discomfort and reduce treatment time. Key words:Collagen membrane, comparative split mouth study, gingival recession, subepithelial connective tissue graft, guided tissue regeneration (GTR).
ABSTRACT
This case report presents a female patient whose chief complaint was of mobile and palatally drifted upper left central incisor which led to malalignment of upper anterior teeth. Orthodontic treatment of upper left central incisor was done with the help of 'Z' spring for the alignment of the upper anterior teeth. It was followed by splinting of upper anterior teeth to improve the stability and masticatory comfort. Regenerative periodontal surgery with Decalcified freeze dried bone allograft was done in relation to upper left central incisor.
ABSTRACT
Papillon-lefevre syndrome (PLS) belongs to a heterogeneous group of skin diseases that are characterized by hyperkeratosis of palms and soles. It is a type IV palmoplantar keratosis (PPK) while the palmoplantar keratodermas share some features of PPK, they are etiologically heterogeneous. PLS differs from other types of PPK by the presence of severe and early onset periodontitis. Genetic studies have shown that mutation in the major gene locus of chromosome 11q14 with the loss of function of cathepsin-C (CTSC) gene is responsible for PLS. CTSC gene mutations are causative for PLS. The resultant loss of CTSC function is responsible for the severe periodontal destruction seen clinically. This report represents two siblings with classical signs and symptoms of PLS.
