Immunological evidence of chemotherapeutic action of mebendazole against Ancylostoma ceylanicum (Looss, 1911) in hamsters (Mesocricetus auratus).

The immunological responses in hamsters during treatment with mebendazole against Ancylostoma ceylanicum are studied. Indirect haemagglutination (IHA) counterimmunoelectrophoresis (CIEP), passive cutaneous anaphylaxis (PCA), foot pad swelling for immediate (ITH) and delayed (DTH) types of hypersensitivities were employed for measuring the responses. Serum antibody which was 1:32 before treatment increased to the maximum of 1:512 (control 1:128) on the 10th day and it declined subsequently. The CIEP test was positive for 10 days and then became negative. The PCA test was positive throughout the observation period both in the treated and untreated groups. Foot pad swelling for ITH and DTH responses were comparatively more prominent than in the untreated control up to the 20th day and then both decreased simultaneously. The immunological responses remained prominent for a longer period and decreased more slowly in the untreated control group.

Inhibition of foot pad multiplication of M. leprae in mice vaccinated with leproma derived M. leprae precursor coccoid organism and passive transfer of DTH response to normal mice through infusion of splenic cells of such vaccinated mice

Inhibition of foot pad multiplication of M. leprae in mice vaccinated with leproma derived M. leprae precursor coccoid organism and passive transfer of DTH response to normal mice through infusion of splenic cells of such vaccinated mice.

N'N'-disubstituted piperazine derivatives as antifilarial antiamoebic and spermicidal agents.

PIP: 4 derivatives of N',N'-piperazine were tested for spectra of activity against filaria, amoeba, and sperm, in addition to bacteria. All 4 of the desubstituted piperazines stopped movements of sperms within 10 minutes at a concentration of 15.6 mg/ml. 2 of the compounds, coded R-3504 and R-3536, showed activity within 2 minutes. Antifilarial activity was exhibited by all 4 compounds within 5 minutes at 15.6 and 31.2 mg/ml against L. carinii and W. bancroft, respectively, in vitro. 3 of the compounds showed activity against E. histolytica at 5 mg/ml; and R-2916 showed antiamoebal activity at 50 mg/ml in vitro. Up to 200 mg/ml, all 4 compounds exhibited no antibacterial activity against S. typhi, S. aureus, and V. cholerae when tested in vitro. The compounds R-2768, R-3504, and R-3536 inhibited the motility of the microfilariae of W. bancrofti and L. carinii; E. histolyica; and human sperms. This was not due to cytotoxic activity but was specific activity since the compounds did not exhibit any antibacterial activity.^ieng

Hydnocarpus oil as an antileprotic agent in footpad technique.

Hydnocarpus oil alone and mixed with dapsone in food and fed to the mice infected with Mycobacterium leprae; showed inhibition of the growth of the lepra bacilli, both sensitive and resistant to dapsone. There was an additive inhibitory effect of the combination of dapsone and oil on the growth of bacilli.

The infectivity of drug resistant cases.

The present study shows that leprosy bacilli resistant to dapsone, multiply in mouse foot-pad as equally as the dapsone sensitive bacilli would multiply suggesting that the dapsone resistant case will be as infective as the dapsone sensitive case.

A preliminary clinical trial of rifampicin in leprosy.

A single dose of 1500 mg. of rifampicin failed to make the lepromatous leprosy patients non-infective by using mouse foot-pad technique. It is essential to extend the trial further.